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Exploring the Role of Nuclear Envelope Proteins in Force Transmission, Nuclear Mechanics, and Muscular Disease.

Record Type:	Language materials, manuscript : Monograph/item
Title/Author:	Exploring the Role of Nuclear Envelope Proteins in Force Transmission, Nuclear Mechanics, and Muscular Disease./
Author:	Fedorchak, Gregory Ryan.
Description:	1 online resource (212 pages)
Notes:	Source: Dissertations Abstracts International, Volume: 79-12, Section: B.
Contained By:	Dissertations Abstracts International79-12B.
Subject:	Cellular biology. -
Online resource:	click for full text (PQDT)
ISBN:	9780438025677

Exploring the Role of Nuclear Envelope Proteins in Force Transmission, Nuclear Mechanics, and Muscular Disease.
Fedorchak, Gregory Ryan.

Exploring the Role of Nuclear Envelope Proteins in Force Transmission, Nuclear Mechanics, and Muscular Disease. - 1 online resource (212 pages)

Source: Dissertations Abstracts International, Volume: 79-12, Section: B.

Thesis (Ph.D.)--Cornell University, 2018.

Includes bibliographical references

Proteins associated with the nucleus' double lipid membrane system (i.e., nuclear envelope proteins) have gained increasing attention in recent years. The bulk of my dissertation work focuses on the development and application of technologies to quantify the contribution of nuclear envelope proteins to the mechanical wiring within living cells. These powerful assays enable measurements of nuclear deformations, nuclear movements (e.g., during muscle differentiation) and nucleo-cytoskeletal force transmission in response to both intra- and extracellular-generated forces. Diseases caused by mutations in genes encoding nuclear envelope proteins, such as Emery-Dreifuss muscular dystrophy, often have convoluted mechanisms due to the multifaceted role of the nuclear envelope in a variety of biochemical and mechanical cellular processes. Therefore, the assays presented herein are particularly valuable in determining the relative contribution of certain proteins to cellular health and disease when they are absent or mutated. While much of my work supports the idea that that mutations in nuclear envelope proteins cause mechanical defects that render the nucleus less stable and more susceptible to physical damage that results in disease, I have also begun investigating whether nuclear envelope proteins can instantaneously translate mechanical force into transcriptional changes, thus addressing a major question in the field of mechanobiology.

Electronic reproduction.
Ann Arbor, Mich. :
ProQuest,
2024

Mode of access: World Wide Web

ISBN: 9780438025677Subjects--Topical Terms:

1148666
Cellular biology.
Subjects--Index Terms:

Cell signalingIndex Terms--Genre/Form:

554714
Electronic books.

Exploring the Role of Nuclear Envelope Proteins in Force Transmission, Nuclear Mechanics, and Muscular Disease.
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502 $a Thesis (Ph.D.)--Cornell University, 2018.
504 $a Includes bibliographical references
520 $a Proteins associated with the nucleus' double lipid membrane system (i.e., nuclear envelope proteins) have gained increasing attention in recent years. The bulk of my dissertation work focuses on the development and application of technologies to quantify the contribution of nuclear envelope proteins to the mechanical wiring within living cells. These powerful assays enable measurements of nuclear deformations, nuclear movements (e.g., during muscle differentiation) and nucleo-cytoskeletal force transmission in response to both intra- and extracellular-generated forces. Diseases caused by mutations in genes encoding nuclear envelope proteins, such as Emery-Dreifuss muscular dystrophy, often have convoluted mechanisms due to the multifaceted role of the nuclear envelope in a variety of biochemical and mechanical cellular processes. Therefore, the assays presented herein are particularly valuable in determining the relative contribution of certain proteins to cellular health and disease when they are absent or mutated. While much of my work supports the idea that that mutations in nuclear envelope proteins cause mechanical defects that render the nucleus less stable and more susceptible to physical damage that results in disease, I have also begun investigating whether nuclear envelope proteins can instantaneously translate mechanical force into transcriptional changes, thus addressing a major question in the field of mechanobiology.
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653 $a Nuclear envelope
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