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Isolation and Molecular Characterization of Pectinase from Penicillium Chrysogenum and Aspergillus Niger Grown on Some Agro Wastes in Lagos, Nigeria.

Record Type:	Language materials, printed : Monograph/item
Title/Author:	Isolation and Molecular Characterization of Pectinase from Penicillium Chrysogenum and Aspergillus Niger Grown on Some Agro Wastes in Lagos, Nigeria./
Author:	Okafor, Uzoma Anthony.
Published:	Ann Arbor : ProQuest Dissertations & Theses, : 2014,
Description:	146 p.
Notes:	Source: Dissertations Abstracts International, Volume: 84-02, Section: B.
Contained By:	Dissertations Abstracts International84-02B.
Subject:	Feeds. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=29216875
ISBN:	9798841507499

Isolation and Molecular Characterization of Pectinase from Penicillium Chrysogenum and Aspergillus Niger Grown on Some Agro Wastes in Lagos, Nigeria.
Okafor, Uzoma Anthony.

Isolation and Molecular Characterization of Pectinase from Penicillium Chrysogenum and Aspergillus Niger Grown on Some Agro Wastes in Lagos, Nigeria. - Ann Arbor : ProQuest Dissertations & Theses, 2014 - 146 p.

Source: Dissertations Abstracts International, Volume: 84-02, Section: B.

Thesis (Ph.D.)--University of Lagos (Nigeria), 2014.

This item must not be sold to any third party vendors.

Waste generated by several agricultural activities has many alternative uses. Unfortunately, much of it is burnt in the open fields, resulting in environmental pollution. In view of the economic and ecological importance of agro wastes, microfungi were isolated from decaying agro wastes samples and screened for pectinolytic activity using a plate assay method containing pectin alone. The effects of carbon, nitrogenous sources and pH on pectinase production by the strains with maximum pectinolytic activities were investigated. Hyper- production of pectinase by these strains using ultra-violet irradiation (UV) as mutagen was also investigated. The selected mutants of Penicillium chrysogenum and Aspergillus niger were characterized using catabolite repression. The genetic variability between the wild and mutant strains was investigated using Random Amplified Polymorphic DNA (RAPD) primers. The polygalacturonase (Pga) produced by the mutant strains were partially purified and the enzyme properties were investigated. Five fungi strains were isolated they are: Aspergillus niger, Aspergillus clavetus, Penicillium chrysogenum, Fusarium species and Trichoderma harzianum. Pectinase production by these isolates showed that the highest pectinase activity index was observed in Aspergillus niger (A.n) followed by Penicillium chrysogenum (P.c). The growth profile of the A.n and P.c in various agro wastes studied showed that these fungi were able to grow in both simple sugar (metabolites) and various agro wastes. The production of pectinase by A.n and P.c in various agro wastes studied showed that wheat bran medium supported pectinase production better than other agro wastes used with a yield of 350.48 IU/ mg protein and 450 IU/ mg protein respectively with maximum yield at 48 h. The inclusion of various nitrogenous salts revealed that urea best supported the production of pectinase (195.75 IU/ mg protein) while polygalacturonase (Pga) production by the addition of urea (33.52 IU/ mg protein) was highly reduced. When P.c was used as the fermenting organism, pectinase production was supported best by peptone 177.97 IU/ mg protein but its polygalacturonase activity was highly reduced (40.35 IU/ mg Protein). Pectinase and polygalacturonase optimization by P.c was best achieved when urea was used as nitrogen source, 124.26 IU/ mg protein for pectinase and 93.00 IU/ mg protein for polygalacturonase after 72 h post fermentation. The stimulation of polygalacturonase and pectinase in A.n using various agro wastes, revealed that sugar cane (73.40 IU/mg protein) and wheat bran (181.43 IU/mg protein) best sustained polygalacturonase and pectinase production respectively and when P.c was induced for both polygalacturonase and pectinase production, wheat bran best supported the production of both polygalacturonase and pectinase. The outcome of pH effect on pectinase production showed an optimal pH of 3.5 and 5.5 for A.n and P.c respectively. Three 2 deoxy-D- glucose (2DG) UV mutants of Aspergillus niger and five 2DG UV mutants of P. chrysogenum were isolated post exposure of the strains to UV irradiation. The comparison of the pectinase production by the suspected mutants and wild strains revealed that; 2 DG UV M2 mutant of Aspergillus niger was selected as the best mutant with enzyme activities of 465% and 230% in solid state fermentation (SSF) and submerged fermentation (SmF) respectively, using wheat bran as the sole carbon source.

ISBN: 9798841507499Subjects--Topical Terms:

590532
Feeds.

Isolation and Molecular Characterization of Pectinase from Penicillium Chrysogenum and Aspergillus Niger Grown on Some Agro Wastes in Lagos, Nigeria.
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