國立虎尾科技大學 |

Synthesis and Biological Evaluation of Pyrrole–Imidazole Polyamide Probes for Visualization of Telomeres

紀錄類型:	書目-語言資料,印刷品 : Monograph/item
正題名/作者:	Synthesis and Biological Evaluation of Pyrrole–Imidazole Polyamide Probes for Visualization of Telomeres/ by Yusuke Kawamoto.
作者:	Kawamoto, Yusuke.
面頁冊數:	XII, 122 p. 62 illus., 52 illus. in color.online resource. :
Contained By:	Springer Nature eBook
標題:	Bioorganic chemistry. -
電子資源:	https://doi.org/10.1007/978-981-13-6912-4
ISBN:	9789811369124

Synthesis and Biological Evaluation of Pyrrole–Imidazole Polyamide Probes for Visualization of Telomeres
Kawamoto, Yusuke.

Synthesis and Biological Evaluation of Pyrrole–Imidazole Polyamide Probes for Visualization of Telomeres[electronic resource] /by Yusuke Kawamoto. - 1st ed. 2019. - XII, 122 p. 62 illus., 52 illus. in color.online resource. - Springer Theses, Recognizing Outstanding Ph.D. Research,2190-5053. - Springer Theses, Recognizing Outstanding Ph.D. Research,.

1. General Introduction Sequence-specific DNA Binding Pyrrole–Imidazole Polyamides and Their Applications -- 2. Development of a New Method for Synthesis of Tandem Hairpin Pyrrole–Imidazole Polyamide Probes Targeting Human Telomeres -- 3. Tandem Trimer Pyrrole-Imidazole Polyamide Probes Targeting 18 Base Pairs in Human Telomere Sequences -- 4. Targeting 24 bp within Telomere Repeat Sequences with Tandem Tetramer Pyrrole−Imidazole Polyamide Probes. .

This book presents a novel method of synthesizing sequence-specific DNA binding pyrrole–imidazole (Py–Im) polyamides and demonstrates polyamides targeting longer base pairs than those previously reported and their cellular application. It discusses the development of the facile synthetic method of tandem hairpin Py–Im polyamides and the method of visualizing telomeres under mild conditions without harsh denaturation, as well as research resulting in the synthesis not only of previously reported tandem dimer polyamides but also longer tandem trimer and tetramer polyamides. Using fluorescently labeled tandem hairpin Py–Im polyamides targeting a human telomere repetitive sequence, the author successfully stained telomeres in fixed cells without denaturation. These fluorescently labeled tandem tetramer polyamides are able to visualize human telomeres with lower background signals than previous polyamide probes, suggesting that they have higher specificity for telomeres. As such, this book provides insights into recent advance in Py–Im polyamides, particularly the extension of the target sites and improvement of the specificity, which can be applied to the study of pyrrole–imidazole polyamides with higher sequence specificity and the function of telomeres.

ISBN: 9789811369124

Standard No.: 10.1007/978-981-13-6912-4doiSubjects--Topical Terms:

743635
Bioorganic chemistry.

LC Class. No.: QD241-441

Dewey Class. No.: 547

Synthesis and Biological Evaluation of Pyrrole–Imidazole Polyamide Probes for Visualization of Telomeres
LDR:03237nam a22004095i 4500 001 1011894
003 DE-He213
005 20200701224728.0
007 cr nn 008mamaa
008 210106s2019 si | s |||| 0|eng d
020 $a 9789811369124 $9 978-981-13-6912-4
024 7 $a 10.1007/978-981-13-6912-4 $2 doi
035 $a 978-981-13-6912-4
050 4 $a QD241-441
072 7 $a PSB $2 bicssc
072 7 $a SCI013040 $2 bisacsh
072 7 $a PSB $2 thema
082 0 4 $a 547 $2 23
100 1 $a Kawamoto, Yusuke. $e author. $4 aut $4 http://id.loc.gov/vocabulary/relators/aut $3 1306134
245 1 0 $a Synthesis and Biological Evaluation of Pyrrole–Imidazole Polyamide Probes for Visualization of Telomeres $h [electronic resource] / $c by Yusuke Kawamoto.
250 $a 1st ed. 2019.
264 1 $a Singapore : $b Springer Singapore : $b Imprint: Springer, $c 2019.
300 $a XII, 122 p. 62 illus., 52 illus. in color. $b online resource.
336 $a text $b txt $2 rdacontent
337 $a computer $b c $2 rdamedia
338 $a online resource $b cr $2 rdacarrier
347 $a text file $b PDF $2 rda
490 1 $a Springer Theses, Recognizing Outstanding Ph.D. Research, $x 2190-5053
505 0 $a 1. General Introduction Sequence-specific DNA Binding Pyrrole–Imidazole Polyamides and Their Applications -- 2. Development of a New Method for Synthesis of Tandem Hairpin Pyrrole–Imidazole Polyamide Probes Targeting Human Telomeres -- 3. Tandem Trimer Pyrrole-Imidazole Polyamide Probes Targeting 18 Base Pairs in Human Telomere Sequences -- 4. Targeting 24 bp within Telomere Repeat Sequences with Tandem Tetramer Pyrrole−Imidazole Polyamide Probes. .
520 $a This book presents a novel method of synthesizing sequence-specific DNA binding pyrrole–imidazole (Py–Im) polyamides and demonstrates polyamides targeting longer base pairs than those previously reported and their cellular application. It discusses the development of the facile synthetic method of tandem hairpin Py–Im polyamides and the method of visualizing telomeres under mild conditions without harsh denaturation, as well as research resulting in the synthesis not only of previously reported tandem dimer polyamides but also longer tandem trimer and tetramer polyamides. Using fluorescently labeled tandem hairpin Py–Im polyamides targeting a human telomere repetitive sequence, the author successfully stained telomeres in fixed cells without denaturation. These fluorescently labeled tandem tetramer polyamides are able to visualize human telomeres with lower background signals than previous polyamide probes, suggesting that they have higher specificity for telomeres. As such, this book provides insights into recent advance in Py–Im polyamides, particularly the extension of the target sites and improvement of the specificity, which can be applied to the study of pyrrole–imidazole polyamides with higher sequence specificity and the function of telomeres.
650 0 $a Bioorganic chemistry. $3 743635
650 0 $a Nucleic acids. $3 788506
650 1 4 $a Bioorganic Chemistry. $3 677511
650 2 4 $a Nucleic Acid Chemistry. $3 716485
710 2 $a SpringerLink (Online service) $3 593884
773 0 $t Springer Nature eBook
776 0 8 $i Printed edition: $z 9789811369117
776 0 8 $i Printed edition: $z 9789811369131
776 0 8 $i Printed edition: $z 9789811369148
830 0 $a Springer Theses, Recognizing Outstanding Ph.D. Research, $x 2190-5053 $3 1253569
856 4 0 $u https://doi.org/10.1007/978-981-13-6912-4
912 $a ZDB-2-CMS
912 $a ZDB-2-SXC
950 $a Chemistry and Materials Science (SpringerNature-11644)
950 $a Chemistry and Material Science (R0) (SpringerNature-43709)