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One-Step Generation of a Drug-Releasing Microarray for High-Throughput Small-Volume Bioassays

Record Type:	Language materials, printed : Monograph/item
Title/Author:	One-Step Generation of a Drug-Releasing Microarray for High-Throughput Small-Volume Bioassays/ by Seo Woo Song.
Author:	Song, Seo Woo.
Description:	XXI, 52 p. 46 illus., 40 illus. in color.online resource. :
Contained By:	Springer Nature eBook
Subject:	Biomedical engineering. -
Online resource:	https://doi.org/10.1007/978-981-13-8094-5
ISBN:	9789811380945

One-Step Generation of a Drug-Releasing Microarray for High-Throughput Small-Volume Bioassays
Song, Seo Woo.

One-Step Generation of a Drug-Releasing Microarray for High-Throughput Small-Volume Bioassays[electronic resource] /by Seo Woo Song. - 1st ed. 2019. - XXI, 52 p. 46 illus., 40 illus. in color.online resource. - Springer Theses, Recognizing Outstanding Ph.D. Research,2190-5053. - Springer Theses, Recognizing Outstanding Ph.D. Research,.

Abstract -- Table of Contents -- List of Tables -- List of Figures -- Chapter 1. Introduction -- 1.1. High-Throughput Small-Volume Bioassays -- 1.2. Developmental Goal for the ‘Pipetting-Free’ HTS Platforms -- 1.3. Main Concept: One-Step Generation of a Drug-Releasing Microarray-on-a-Chip by Self-Assembly of Drug-Laden Microparticles (DLPs) -- Chapter 2. System Development -- 2.1. Sealing-Film Assisted Seeding Method for Saving Cell Consumptions -- 2.2. Chip and Jig Development -- 2.3. Preparation of DLPs Library -- 2.4. Decoding Microparticles -- 2.5. Statistical Analysis for Duplications -- Chapter 3. Application: Screening of Sequential Drug Combinations -- 3.1. Therapeutic Benefit of Sequential Drug Combination Based on Rewiring of Intracellular Pathways -- 3.2. Screening of Sequential Drug Combination Using a Partipetting Platform -- 3.3. Proof-of-Concept: Sequential Combinatorial Cell Staining Assay by Replacement of the Drug Chip -- 3.4. Screening of Sequential Combinatorial Drugs with EGFR Inhibitor Followed by Genotoxin against Triple Negative Breast Cancer (TNBC) -- Chapter 4. Conclusion and Discussion -- Bibliography.

This thesis demonstrates a technology that enables pipetting-free high-throughput screening (HTS) on a miniaturized platform, eliminating the need for thousands of one-by-one pipetting and conventional liquid handling systems. This platform enhances accessibility to HTS and enables HTS to be used in small-to-medium scale laboratories. In addition, it allows large-scale combinatorial screening with a small number of valuable cells, such as patients’ primary cancer cells. This technique will have a high impact for widespread use of HTS in the era of personalized medicine. In this thesis, the author firstly describes the need and concept of ‘partipetting’ for pipetting-free HTS platform. It is realized by the one-step pipetting and self-assembly of encoded drug-laden microparticles (DLPs) on the microwells. Next, the technical implementations required for the platform demonstration are described. It includes preparation of encoded DLPs, plastic chip fabrication, and realization of automated system. Lastly, screening of sequential drug combinations using this platform is demonstrated. This shows the potential of the proposed technology for various applications.

ISBN: 9789811380945

Standard No.: 10.1007/978-981-13-8094-5doiSubjects--Topical Terms:

588770
Biomedical engineering.

LC Class. No.: R856-857

Dewey Class. No.: 610.28

One-Step Generation of a Drug-Releasing Microarray for High-Throughput Small-Volume Bioassays
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