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The Protective Effects of Natural Antioxidants on Ionizing Radiation-Induced Apoptosis and Cell Cycle Arrest in Human Intestinal Epithelial Cells.

Record Type:	Language materials, printed : Monograph/item
Title/Author:	The Protective Effects of Natural Antioxidants on Ionizing Radiation-Induced Apoptosis and Cell Cycle Arrest in Human Intestinal Epithelial Cells./
Author:	Alshihri, Abdulaziz.
Published:	Ann Arbor : ProQuest Dissertations & Theses, : 2020,
Description:	90 p.
Notes:	Source: Dissertations Abstracts International, Volume: 81-12, Section: B.
Contained By:	Dissertations Abstracts International81-12B.
Subject:	Biomedical engineering. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=27961063
ISBN:	9798635278031

The Protective Effects of Natural Antioxidants on Ionizing Radiation-Induced Apoptosis and Cell Cycle Arrest in Human Intestinal Epithelial Cells.
Alshihri, Abdulaziz.

The Protective Effects of Natural Antioxidants on Ionizing Radiation-Induced Apoptosis and Cell Cycle Arrest in Human Intestinal Epithelial Cells. - Ann Arbor : ProQuest Dissertations & Theses, 2020 - 90 p.

Source: Dissertations Abstracts International, Volume: 81-12, Section: B.

Thesis (Ph.D.)--University of Massachusetts Lowell, 2020.

This item must not be sold to any third party vendors.

The growing use of ionizing radiation and radioactive substances in society causes an increase in human exposure, in addition to the exposure from natural radiation sources. Ionizing radiation can cause adverse biological effects in living cells, such as damage to DNA, which is a critical target in radiation exposures. Substances such as antioxidants that scavenge free radicals and reactive oxygen species can play a significant role in reducing the harmful effects of radiation exposures. This dissertation study examined the protective effects of natural antioxidants as radioprotectors (a countermeasure administered prior to radiation exposure) or as radiomitigators (a countermeasure administered after radiation exposure). The antioxidants that were investigated in this research are vitamin C and thymoquinone and their combination. The efficacy of these compounds as radiation countermeasures was based on the inhibition of apoptosis through the mitochondria pathway, the induction of cell cycle arrest, and enhancing DNA repair for human intestinal epithelial cells (HIEC) exposed to x rays and mixed neutron-gamma radiations. These results were determined using MTS assay, a caspase activity assay, annexin V/propidium iodide assay, and cell cycle analysis. The findings of this study showed that the combination of vitamin C and thymoquinone proved to be an effective radioprotector and radiomitigator against low LET radiation. The combination was also proved to be effective as a radiomitigator in case of high-LET radiation.

ISBN: 9798635278031Subjects--Topical Terms:

588770
Biomedical engineering.
Subjects--Index Terms:

Ionizing radiation

The Protective Effects of Natural Antioxidants on Ionizing Radiation-Induced Apoptosis and Cell Cycle Arrest in Human Intestinal Epithelial Cells.
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500 $a Source: Dissertations Abstracts International, Volume: 81-12, Section: B.
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506 $a This item must not be sold to any third party vendors.
520 $a The growing use of ionizing radiation and radioactive substances in society causes an increase in human exposure, in addition to the exposure from natural radiation sources. Ionizing radiation can cause adverse biological effects in living cells, such as damage to DNA, which is a critical target in radiation exposures. Substances such as antioxidants that scavenge free radicals and reactive oxygen species can play a significant role in reducing the harmful effects of radiation exposures. This dissertation study examined the protective effects of natural antioxidants as radioprotectors (a countermeasure administered prior to radiation exposure) or as radiomitigators (a countermeasure administered after radiation exposure). The antioxidants that were investigated in this research are vitamin C and thymoquinone and their combination. The efficacy of these compounds as radiation countermeasures was based on the inhibition of apoptosis through the mitochondria pathway, the induction of cell cycle arrest, and enhancing DNA repair for human intestinal epithelial cells (HIEC) exposed to x rays and mixed neutron-gamma radiations. These results were determined using MTS assay, a caspase activity assay, annexin V/propidium iodide assay, and cell cycle analysis. The findings of this study showed that the combination of vitamin C and thymoquinone proved to be an effective radioprotector and radiomitigator against low LET radiation. The combination was also proved to be effective as a radiomitigator in case of high-LET radiation.
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