國立虎尾科技大學 |

Cancer Biomarker Detection in Human Plasma by Integrating Surface Acoustic Wave and Metal-Enhanced Fluorescence.

紀錄類型:	書目-語言資料,印刷品 : Monograph/item
正題名/作者:	Cancer Biomarker Detection in Human Plasma by Integrating Surface Acoustic Wave and Metal-Enhanced Fluorescence./
作者:	Huang, Yuqi.
出版者:	Ann Arbor : ProQuest Dissertations & Theses, : 2020,
面頁冊數:	49 p.
附註:	Source: Masters Abstracts International, Volume: 82-01.
Contained By:	Masters Abstracts International82-01.
標題:	Materials science. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=28001345
ISBN:	9798662464469

Cancer Biomarker Detection in Human Plasma by Integrating Surface Acoustic Wave and Metal-Enhanced Fluorescence.
Huang, Yuqi.

Cancer Biomarker Detection in Human Plasma by Integrating Surface Acoustic Wave and Metal-Enhanced Fluorescence. - Ann Arbor : ProQuest Dissertations & Theses, 2020 - 49 p.

Source: Masters Abstracts International, Volume: 82-01.

Thesis (M.S.M.S.E.)--University of South Florida, 2020.

This item must not be sold to any third party vendors.

This thesis includes data and discussion about the technique of metal-enhanced fluorescence (MEF) to lower the detection limit of carcinoembryonic antigen (CEA). The detection limit goes down to 100pg/mL level when using MEF substrate made by rapid thermally annealed silver film covered by silica, which has great promise in diagnosing certain types of cancer that uses CEA as detection biomarker, such as pancreatic cancer and colon cancer. To further address the issue of background noises from non-specifically bound proteins (NSB) in complex media, such as plasma, serum, urine and blood, MEF is integrated with surface acoustic wave (SAW) streaming in order to remove these proteins from human plasma while keeping the characteristic of MEF to increase signal to detect target analyte. SAW has also been proven to have the effect of reducing incubation time between antibody and target antigen. Analyzing fluorescence signal shows that the NSB in human plasma can be successfully reduced while the CEA detection limit can be lowered down to 500pg/mL by combining MEF with SAW, allowing for construction of a point of need sensing platform for biomarkers from such complex fluids.

ISBN: 9798662464469Subjects--Topical Terms:

557839
Materials science.
Subjects--Index Terms:

CEA

Cancer Biomarker Detection in Human Plasma by Integrating Surface Acoustic Wave and Metal-Enhanced Fluorescence.
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300 $a 49 p.
500 $a Source: Masters Abstracts International, Volume: 82-01.
500 $a Advisor: Bhethanabotla, Venkat.
502 $a Thesis (M.S.M.S.E.)--University of South Florida, 2020.
506 $a This item must not be sold to any third party vendors.
520 $a This thesis includes data and discussion about the technique of metal-enhanced fluorescence (MEF) to lower the detection limit of carcinoembryonic antigen (CEA). The detection limit goes down to 100pg/mL level when using MEF substrate made by rapid thermally annealed silver film covered by silica, which has great promise in diagnosing certain types of cancer that uses CEA as detection biomarker, such as pancreatic cancer and colon cancer. To further address the issue of background noises from non-specifically bound proteins (NSB) in complex media, such as plasma, serum, urine and blood, MEF is integrated with surface acoustic wave (SAW) streaming in order to remove these proteins from human plasma while keeping the characteristic of MEF to increase signal to detect target analyte. SAW has also been proven to have the effect of reducing incubation time between antibody and target antigen. Analyzing fluorescence signal shows that the NSB in human plasma can be successfully reduced while the CEA detection limit can be lowered down to 500pg/mL by combining MEF with SAW, allowing for construction of a point of need sensing platform for biomarkers from such complex fluids.
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