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Super-Resolution Microscopy Development for the Detection of Nano-Structures and Confocal Study of the Structural Damage in Gut Cell Nuclei Due to Total Body Irradiation.

Record Type:	Language materials, printed : Monograph/item
Title/Author:	Super-Resolution Microscopy Development for the Detection of Nano-Structures and Confocal Study of the Structural Damage in Gut Cell Nuclei Due to Total Body Irradiation./
Author:	Hasan, Mehedi.
Published:	Ann Arbor : ProQuest Dissertations & Theses, : 2020,
Description:	63 p.
Notes:	Source: Masters Abstracts International, Volume: 82-06.
Contained By:	Masters Abstracts International82-06.
Subject:	Molecular physics. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=28155833
ISBN:	9798698591429

Super-Resolution Microscopy Development for the Detection of Nano-Structures and Confocal Study of the Structural Damage in Gut Cell Nuclei Due to Total Body Irradiation.
Hasan, Mehedi.

Super-Resolution Microscopy Development for the Detection of Nano-Structures and Confocal Study of the Structural Damage in Gut Cell Nuclei Due to Total Body Irradiation. - Ann Arbor : ProQuest Dissertations & Theses, 2020 - 63 p.

Source: Masters Abstracts International, Volume: 82-06.

Thesis (M.S.)--Mississippi State University, 2020.

This item must not be sold to any third party vendors.

Optical microscopy is the oldest form of microscopy that has been visually aiding scientific research. In our research, I have reported here two such optical microscopy techniques for two different projects. In the first project, we re-developed an instrumentation of a cost-effective, high-performing, single-molecular localization super-resolution microscopy setup that breaks the diffraction limitation barrier. Then we use a stochastic image capturing technique to capture the best precision image positions of gold nanoparticles. In our second project, we apply confocal microscopy technique to image DNA molecular nanoscale structural alterations of chromatin in cell nuclei of gut tissues caused by total body irradiation (TBI). We then quantify these alterations using a light localization technique called inverse participation ratio (IPR) using the confocal micrographs of the sample. Our results show radiation causes reduction and saturation of DNA spatial mass density fluctuations that were observed for different durations of post-irradiation.

ISBN: 9798698591429Subjects--Topical Terms:

1181248
Molecular physics.
Subjects--Index Terms:

Confocal microscopy

Super-Resolution Microscopy Development for the Detection of Nano-Structures and Confocal Study of the Structural Damage in Gut Cell Nuclei Due to Total Body Irradiation.
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245 1 0 $a Super-Resolution Microscopy Development for the Detection of Nano-Structures and Confocal Study of the Structural Damage in Gut Cell Nuclei Due to Total Body Irradiation.
260 1 $a Ann Arbor : $b ProQuest Dissertations & Theses, $c 2020
300 $a 63 p.
500 $a Source: Masters Abstracts International, Volume: 82-06.
500 $a Advisor: Pradhan, Prabhakar.
502 $a Thesis (M.S.)--Mississippi State University, 2020.
506 $a This item must not be sold to any third party vendors.
520 $a Optical microscopy is the oldest form of microscopy that has been visually aiding scientific research. In our research, I have reported here two such optical microscopy techniques for two different projects. In the first project, we re-developed an instrumentation of a cost-effective, high-performing, single-molecular localization super-resolution microscopy setup that breaks the diffraction limitation barrier. Then we use a stochastic image capturing technique to capture the best precision image positions of gold nanoparticles. In our second project, we apply confocal microscopy technique to image DNA molecular nanoscale structural alterations of chromatin in cell nuclei of gut tissues caused by total body irradiation (TBI). We then quantify these alterations using a light localization technique called inverse participation ratio (IPR) using the confocal micrographs of the sample. Our results show radiation causes reduction and saturation of DNA spatial mass density fluctuations that were observed for different durations of post-irradiation.
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650 4 $a Radiation. $3 673044
650 4 $a Nanoscience. $3 632473
653 $a Confocal microscopy
653 $a Gold nanoparticles
653 $a Single molecule localization Microscopy
653 $a Structural disorder
653 $a Super resolution microscopy
653 $a Gut cell nuclei
653 $a Total body irradiation
653 $a SNA spatial mass
653 $a Density fluctuations
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