國立虎尾科技大學 |

African Swine Fever Virus = Methods and Protocols /

Record Type:	Language materials, printed : Monograph/item
Title/Author:	African Swine Fever Virus/ edited by Christopher L. Netherton.
Reminder of title:	Methods and Protocols /
other author:	Netherton, Christopher L.
Description:	XII, 218 p. 44 illus., 41 illus. in color.online resource. :
Contained By:	Springer Nature eBook
Subject:	Virology. -
Online resource:	https://doi.org/10.1007/978-1-0716-2333-6
ISBN:	9781071623336

African Swine Fever Virus = Methods and Protocols /
African Swine Fever VirusMethods and Protocols /[electronic resource] :edited by Christopher L. Netherton. - 1st ed. 2022. - XII, 218 p. 44 illus., 41 illus. in color.online resource. - Methods in Molecular Biology,25031940-6029 ;. - Methods in Molecular Biology,2540.

Working safely with African swine fever virus -- Necropsy procedures and evaluation of macroscopic lesions of pigs infected with African swine fever virus -- Preparation of immunofluorescently labelled tissue sections for imaging at low and high magnifications in the confocal microscope -- Primary macrophage culture from porcine blood and lungs -- Isolation of porcine bone marrow cells and generation of recombinant African swine fever viruses -- Laboratory diagnosis and quantification of African swine fever virus using real-time polymerase chain reaction -- Quantification of ASFV DNA and RNA in Ornithodoros soft ticks -- Genotyping of African swine fever virus -- African swine fever virus (ASFV) indirect ELISA test based on the use of the soluble cytoplasmic semi purified antigen (ASFV CP-Ag) -- Indirect immunoperoxidase test (IPT) for detection of antibodies against African swine fever virus (ASFV) on African green monkey cell lines (Vero, MS) -- African Swine Fever Virus Haemadosrption Inhibition Assay -- ELISpot assay for the detection of ASFV-specific interferon-gamma (IFN-γ) producing cells -- Purification of African swine fever virus -- African swine fever virus plaque assay and disinfectant testing -- Bioorthogonal labelling of African swine fever virus infected cells -- Whole genome sequencing of African swine fever virus.

This volume provides a compilation of methods that will aid researchers studying virology, immunology, and vaccinology of this devastating disease of swine. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, African Swine Fever Virus: Methods and Protocols aims to be a foundation for future studies and to be a source of inspiration for new investigations in the field. .

ISBN: 9781071623336

Standard No.: 10.1007/978-1-0716-2333-6doiSubjects--Topical Terms:

644995
Virology.

LC Class. No.: QR355-502

Dewey Class. No.: 579.2

African Swine Fever Virus = Methods and Protocols /
LDR:03325nam a22003975i 4500 001 1089337
003 DE-He213
005 20220516124825.0
007 cr nn 008mamaa
008 221228s2022 xxu| s |||| 0|eng d
020 $a 9781071623336 $9 978-1-0716-2333-6
024 7 $a 10.1007/978-1-0716-2333-6 $2 doi
035 $a 978-1-0716-2333-6
050 4 $a QR355-502
072 7 $a PSGL $2 bicssc
072 7 $a MED052000 $2 bisacsh
072 7 $a PSG $2 thema
082 0 4 $a 579.2 $2 23
082 0 4 $a 616.96 $2 23
245 1 0 $a African Swine Fever Virus $h [electronic resource] : $b Methods and Protocols / $c edited by Christopher L. Netherton.
250 $a 1st ed. 2022.
264 1 $a New York, NY : $b Springer US : $b Imprint: Humana, $c 2022.
300 $a XII, 218 p. 44 illus., 41 illus. in color. $b online resource.
336 $a text $b txt $2 rdacontent
337 $a computer $b c $2 rdamedia
338 $a online resource $b cr $2 rdacarrier
347 $a text file $b PDF $2 rda
490 1 $a Methods in Molecular Biology, $x 1940-6029 ; $v 2503
505 0 $a Working safely with African swine fever virus -- Necropsy procedures and evaluation of macroscopic lesions of pigs infected with African swine fever virus -- Preparation of immunofluorescently labelled tissue sections for imaging at low and high magnifications in the confocal microscope -- Primary macrophage culture from porcine blood and lungs -- Isolation of porcine bone marrow cells and generation of recombinant African swine fever viruses -- Laboratory diagnosis and quantification of African swine fever virus using real-time polymerase chain reaction -- Quantification of ASFV DNA and RNA in Ornithodoros soft ticks -- Genotyping of African swine fever virus -- African swine fever virus (ASFV) indirect ELISA test based on the use of the soluble cytoplasmic semi purified antigen (ASFV CP-Ag) -- Indirect immunoperoxidase test (IPT) for detection of antibodies against African swine fever virus (ASFV) on African green monkey cell lines (Vero, MS) -- African Swine Fever Virus Haemadosrption Inhibition Assay -- ELISpot assay for the detection of ASFV-specific interferon-gamma (IFN-γ) producing cells -- Purification of African swine fever virus -- African swine fever virus plaque assay and disinfectant testing -- Bioorthogonal labelling of African swine fever virus infected cells -- Whole genome sequencing of African swine fever virus.
520 $a This volume provides a compilation of methods that will aid researchers studying virology, immunology, and vaccinology of this devastating disease of swine. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, African Swine Fever Virus: Methods and Protocols aims to be a foundation for future studies and to be a source of inspiration for new investigations in the field. .
650 0 $a Virology. $3 644995
650 0 $a Zoology. $3 593903
650 0 $a Immunology. $3 592892
700 1 $a Netherton, Christopher L. $e editor. $4 edt $4 http://id.loc.gov/vocabulary/relators/edt $3 1396584
710 2 $a SpringerLink (Online service) $3 593884
773 0 $t Springer Nature eBook
776 0 8 $i Printed edition: $z 9781071623329
776 0 8 $i Printed edition: $z 9781071623343
776 0 8 $i Printed edition: $z 9781071623350
830 0 $a Methods in Molecular Biology, $x 1940-6029 ; $v 2540 $3 1387604
856 4 0 $u https://doi.org/10.1007/978-1-0716-2333-6
912 $a ZDB-2-PRO
950 $a Springer Protocols (Springer-12345)