國立虎尾科技大學 |

Recombineering = Methods and Protocols /

Record Type:	Language materials, printed : Monograph/item
Title/Author:	Recombineering/ edited by Christopher R. Reisch.
Reminder of title:	Methods and Protocols /
other author:	Reisch, Christopher R.
Description:	XI, 264 p. 57 illus., 50 illus. in color.online resource. :
Contained By:	Springer Nature eBook
Subject:	Microbiology. -
Online resource:	https://doi.org/10.1007/978-1-0716-2233-9
ISBN:	9781071622339

Recombineering = Methods and Protocols /
RecombineeringMethods and Protocols /[electronic resource] :edited by Christopher R. Reisch. - 1st ed. 2022. - XI, 264 p. 57 illus., 50 illus. in color.online resource. - Methods in Molecular Biology,24791940-6029 ;. - Methods in Molecular Biology,2540.

Scarless ssDNA Recombineering of Phage in the Lysogenic State -- Lambda red Recombineering of Bacteriophage in the Lysogenic State -- Recombineering Mediated Genome Editing in Burkholderiales Strains -- High-Efficiency Multi-Site Genomic Editing (HEMSE) Made Easy -- Creation of Markerless Genome Modifications in a Non-Model Bacterium by Fluorescence-Aided Recombineering -- Plant Gene Modification by BAC Recombineering -- Dup-In and DIRex: Techniques for Single-Step, Scar-Free Mutagenesis with Marker Recycling -- Genome Editing in Klebsiella pneumoniae using CRISPR/Cas9 Technology -- Generating Single Nucleotide Point Mutations in E. coli with the No-SCAR System -- Recombineering in Staphylococcus aureus -- Genome Editing Methods for Bacillus subtilis -- Highly Efficient Genome Editing in Clostridium difficile using the CRISPR-Cpf1 System -- Genome Editing of Corynebacterium glutamicum using CRISPR-Cpf1 System -- CRISPR/Cas-Mediated Genome Editing of Streptomyces -- Cas9 Nickase-Based Genome Editing in Clostridium cellulolyticum -- Genome Editing with Cas9 in Lactobacilli.

This volume explores a collection of methods that studies genome editing across a variety of bacteria, phages, and plants. Chapters in this book cover topics such as scarless DNA recombineering of phage in the lysogenic state; HEMSE; Dup-In and DIRex; recombineering in Staphylococcus aureus; and genome editing with Cas9 in lactobacilli. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Recombineering: Methods and Protocols is a valuable resource for any researchers interested in learning more about this developing field. .

ISBN: 9781071622339

Standard No.: 10.1007/978-1-0716-2233-9doiSubjects--Topical Terms:

591510
Microbiology.

LC Class. No.: QR

Dewey Class. No.: 579

Recombineering = Methods and Protocols /
LDR:03179nam a22003855i 4500 001 1089913
003 DE-He213
005 20220518091004.0
007 cr nn 008mamaa
008 221228s2022 xxu| s |||| 0|eng d
020 $a 9781071622339 $9 978-1-0716-2233-9
024 7 $a 10.1007/978-1-0716-2233-9 $2 doi
035 $a 978-1-0716-2233-9
050 4 $a QR
072 7 $a PSG $2 bicssc
072 7 $a SCI045000 $2 bisacsh
072 7 $a PSG $2 thema
082 0 4 $a 579 $2 23
245 1 0 $a Recombineering $h [electronic resource] : $b Methods and Protocols / $c edited by Christopher R. Reisch.
250 $a 1st ed. 2022.
264 1 $a New York, NY : $b Springer US : $b Imprint: Humana, $c 2022.
300 $a XI, 264 p. 57 illus., 50 illus. in color. $b online resource.
336 $a text $b txt $2 rdacontent
337 $a computer $b c $2 rdamedia
338 $a online resource $b cr $2 rdacarrier
347 $a text file $b PDF $2 rda
490 1 $a Methods in Molecular Biology, $x 1940-6029 ; $v 2479
505 0 $a Scarless ssDNA Recombineering of Phage in the Lysogenic State -- Lambda red Recombineering of Bacteriophage in the Lysogenic State -- Recombineering Mediated Genome Editing in Burkholderiales Strains -- High-Efficiency Multi-Site Genomic Editing (HEMSE) Made Easy -- Creation of Markerless Genome Modifications in a Non-Model Bacterium by Fluorescence-Aided Recombineering -- Plant Gene Modification by BAC Recombineering -- Dup-In and DIRex: Techniques for Single-Step, Scar-Free Mutagenesis with Marker Recycling -- Genome Editing in Klebsiella pneumoniae using CRISPR/Cas9 Technology -- Generating Single Nucleotide Point Mutations in E. coli with the No-SCAR System -- Recombineering in Staphylococcus aureus -- Genome Editing Methods for Bacillus subtilis -- Highly Efficient Genome Editing in Clostridium difficile using the CRISPR-Cpf1 System -- Genome Editing of Corynebacterium glutamicum using CRISPR-Cpf1 System -- CRISPR/Cas-Mediated Genome Editing of Streptomyces -- Cas9 Nickase-Based Genome Editing in Clostridium cellulolyticum -- Genome Editing with Cas9 in Lactobacilli.
520 $a This volume explores a collection of methods that studies genome editing across a variety of bacteria, phages, and plants. Chapters in this book cover topics such as scarless DNA recombineering of phage in the lysogenic state; HEMSE; Dup-In and DIRex; recombineering in Staphylococcus aureus; and genome editing with Cas9 in lactobacilli. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Recombineering: Methods and Protocols is a valuable resource for any researchers interested in learning more about this developing field. .
650 0 $a Microbiology. $3 591510
650 0 $a Microbial genetics. $3 582864
650 2 4 $a Microbial Genetics. $3 1366868
700 1 $a Reisch, Christopher R. $e editor. $4 edt $4 http://id.loc.gov/vocabulary/relators/edt $3 1397220
710 2 $a SpringerLink (Online service) $3 593884
773 0 $t Springer Nature eBook
776 0 8 $i Printed edition: $z 9781071622322
776 0 8 $i Printed edition: $z 9781071622346
776 0 8 $i Printed edition: $z 9781071622353
830 0 $a Methods in Molecular Biology, $x 1940-6029 ; $v 2540 $3 1387604
856 4 0 $u https://doi.org/10.1007/978-1-0716-2233-9
912 $a ZDB-2-PRO
950 $a Springer Protocols (Springer-12345)