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DNA and RNA Isolation Techniques for Non-Experts

紀錄類型:	書目-語言資料,印刷品 : Monograph/item
正題名/作者:	DNA and RNA Isolation Techniques for Non-Experts/ by Akash Gautam.
作者:	Gautam, Akash.
面頁冊數:	XVI, 203 p. 58 illus., 48 illus. in color.online resource. :
Contained By:	Springer Nature eBook
標題:	Cell Biology. -
電子資源:	https://doi.org/10.1007/978-3-030-94230-4
ISBN:	9783030942304

DNA and RNA Isolation Techniques for Non-Experts
Gautam, Akash.

DNA and RNA Isolation Techniques for Non-Experts[electronic resource] /by Akash Gautam. - 1st ed. 2022. - XVI, 203 p. 58 illus., 48 illus. in color.online resource. - Techniques in Life Science and Biomedicine for the Non-Expert,2367-1122. - Techniques in Life Science and Biomedicine for the Non-Expert,.

1-Basic Idea About Nucleic Acids For Non-Experts -- 2-General Steps During Isolation Of DNA And RNA -- 3-Phenol-Chloroform DNA Isolation Method -- 4-RNA Isolation By The Guanidinium-Acid-Phenol Method -- 5-Spin Column-Based Isolation Of Nucleic Acid -- 6-Isolation Of Plasmid DNA By Alkaline Lysis -- 7-DNA Isolation By Hydrophilic Ionic Liquid Treatment -- 8-Lithium Chloride Based Isolation Of DNA And RNA -- 9-Isolation Of Bacteriophage DNA By Peg Method -- 10-DNA Isolation By Chelex Method -- 11-DNA Isolation By Lysozyme And Proteinase K -- 12-Salting-Out Method For Blood Samples -- 13-CTAB Or SDA Based Isolation Of Plant’s DNA -- 14-DNA Extraction By Spooling Method -- 15-Magnetic Bead-Based Nucleic Acid Isolation -- 16-Density Gradient-Based Nucleic Acid Isolation -- 17-DNA Extraction From Agarose Gel Though Paper-Strips -- 18-Transformation Or Genetic Modification Of Cells/Organisms -- 19-Gene Cloning And Vectors -- 20-Polymerase Chain Reaction (PCR) -- 21-Southern And Northern Blotting -- 22-Genome Mapping -- 23-Applications Of DNA Sequencing Technologies For Current Research.

This thorough introductory volume presents the background, applications, and stepwise directions for standard DNA and RNA isolation techniques. Unlike a kit chemistry approach, this book provides a breadth of information necessary for junior or non-expert researchers to learn and apply these techniques in their work. An accessible, indispensable how-to guide for researchers in immunology, molecular biology, zoology, forensic science, genetics, botany, neuroscience, physiology, and others.

ISBN: 9783030942304

Standard No.: 10.1007/978-3-030-94230-4doiSubjects--Topical Terms:

593889
Cell Biology.

LC Class. No.: QH506

Dewey Class. No.: 572

DNA and RNA Isolation Techniques for Non-Experts
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