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Development of Microfluidic Platforms for Sperm Protein 10 Measurement.

Record Type:	Language materials, printed : Monograph/item
Title/Author:	Development of Microfluidic Platforms for Sperm Protein 10 Measurement./
Author:	Huang, Zongjie.
Published:	Ann Arbor : ProQuest Dissertations & Theses, : 2022,
Description:	124 p.
Notes:	Source: Dissertations Abstracts International, Volume: 84-05, Section: B.
Contained By:	Dissertations Abstracts International84-05B.
Subject:	Mechanical engineering. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=29252974
ISBN:	9798834060185

Development of Microfluidic Platforms for Sperm Protein 10 Measurement.
Huang, Zongjie.

Development of Microfluidic Platforms for Sperm Protein 10 Measurement. - Ann Arbor : ProQuest Dissertations & Theses, 2022 - 124 p.

Source: Dissertations Abstracts International, Volume: 84-05, Section: B.

Thesis (Ph.D.)--University of Toronto (Canada), 2022.

This item must not be sold to any third party vendors.

Infertility is a worldwide disease. Approximately 48.5 million couples worldwide are infertile, including one in six Canadian couples. Male infertility, which affects approximately 7% of the male population, is responsible for an estimated 40% to 50% of all infertility cases. Male infertility is often attributed to poor semen quality with suboptimal sperm motility, limited concentration, or abnormal morphology. The total number of spermatozoa per ejaculate and the sperm concentration are related to both time to pregnancy and pregnancy rates and are predictors of conception. Since sperm concentration is an important criterion to estimate semen quality, a direct relationship between sperm concentration and signal intensity is desired for establishment through enzyme linked immunosorbent assays that measure sperm protein 10 (SP-10) concentration.This thesis focuses on developing three types of microfluidic platforms for SP-10 measurement. Firstly, a nitrocellulose paper-based multi-well plate platform was developed with a LOD of 88.84 ng/ml. The developed nitrocellulose paper plate has 96 independent wells for multiplexing ELISA assay and is compatible with standard laboratory microplates and instruments. The nitrocellulose plate is fabricated through a flexible one-step laser micromachining process, which can simply and rapidly produce a low-cost nitrocellulose paper-based multi-well plate with different sizes for ELISA experiments. Next, a lateral flow immunoassay platform was developed for home-based SP-10 measurement. This simple, rapid, and cost-effective platform replies on capillarity of a nitrocellulose strip to transport fluids and produces a measurable signal within 15 minutes. Gold nanoparticles and gold nanoshells were both applied on this platform as colorimetric labels; it was demonstrated that the LOD with gold nanoshells (5.16 ng/ml) was 5 times lower than that with gold nanoparticles (25.03 ng/ml). In addition, a single magnetic bead system integrated with a microfluidic device was developed for SP-10 measurement in laboratory with a LOD of 1.18 µg/ml. By applying a rotating magnetic field, the single magnetic bead moves freely in the chamber of the microfluidic device to actively capture the SP-10 antigen linked to the fluorescent-labeled SP-10 antibody. SP-10 concentration was measured by comparing the fluorescent intensity of the bead before and after swimming in the chamber.

ISBN: 9798834060185Subjects--Topical Terms:

557493
Mechanical engineering.
Subjects--Index Terms:

Microfluidic platforms

Development of Microfluidic Platforms for Sperm Protein 10 Measurement.
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500 $a Advisor: Sun, Yu.
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506 $a This item is not available from ProQuest Dissertations & Theses.
506 $a This item must not be sold to any third party vendors.
520 $a Infertility is a worldwide disease. Approximately 48.5 million couples worldwide are infertile, including one in six Canadian couples. Male infertility, which affects approximately 7% of the male population, is responsible for an estimated 40% to 50% of all infertility cases. Male infertility is often attributed to poor semen quality with suboptimal sperm motility, limited concentration, or abnormal morphology. The total number of spermatozoa per ejaculate and the sperm concentration are related to both time to pregnancy and pregnancy rates and are predictors of conception. Since sperm concentration is an important criterion to estimate semen quality, a direct relationship between sperm concentration and signal intensity is desired for establishment through enzyme linked immunosorbent assays that measure sperm protein 10 (SP-10) concentration.This thesis focuses on developing three types of microfluidic platforms for SP-10 measurement. Firstly, a nitrocellulose paper-based multi-well plate platform was developed with a LOD of 88.84 ng/ml. The developed nitrocellulose paper plate has 96 independent wells for multiplexing ELISA assay and is compatible with standard laboratory microplates and instruments. The nitrocellulose plate is fabricated through a flexible one-step laser micromachining process, which can simply and rapidly produce a low-cost nitrocellulose paper-based multi-well plate with different sizes for ELISA experiments. Next, a lateral flow immunoassay platform was developed for home-based SP-10 measurement. This simple, rapid, and cost-effective platform replies on capillarity of a nitrocellulose strip to transport fluids and produces a measurable signal within 15 minutes. Gold nanoparticles and gold nanoshells were both applied on this platform as colorimetric labels; it was demonstrated that the LOD with gold nanoshells (5.16 ng/ml) was 5 times lower than that with gold nanoparticles (25.03 ng/ml). In addition, a single magnetic bead system integrated with a microfluidic device was developed for SP-10 measurement in laboratory with a LOD of 1.18 µg/ml. By applying a rotating magnetic field, the single magnetic bead moves freely in the chamber of the microfluidic device to actively capture the SP-10 antigen linked to the fluorescent-labeled SP-10 antibody. SP-10 concentration was measured by comparing the fluorescent intensity of the bead before and after swimming in the chamber.
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