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整合型離心微流體晶片用於全血中無標記癌細胞分離及細胞裂解 = = Int...

劉怡志

整合型離心微流體晶片用於全血中無標記癌細胞分離及細胞裂解 = = Integrated Centrifugal Microfluidic Chip for Label-Free Cancer Cell Separation and Cell Lysis in Whole Blood /

Record Type:	Language materials, printed : Monograph/item
Title/Author:	整合型離心微流體晶片用於全血中無標記癌細胞分離及細胞裂解 =/ 劉怡志.
Reminder of title:	Integrated Centrifugal Microfluidic Chip for Label-Free Cancer Cell Separation and Cell Lysis in Whole Blood /
remainder title:	Integrated Centrifugal Microfluidic Chip for Label-Free Cancer Cell Separation and Cell Lysis in Whole Blood.
Author:	劉怡志
Published:	雲林縣 :國立虎尾科技大學 , : 民113.07.,
Description:	[13], 83面 :圖, 表 ; : 30公分.;
Notes:	指導教授: 郭如男.
Subject:	COMSOL. -
Online resource:	電子資源

整合型離心微流體晶片用於全血中無標記癌細胞分離及細胞裂解 = = Integrated Centrifugal Microfluidic Chip for Label-Free Cancer Cell Separation and Cell Lysis in Whole Blood /
劉怡志

整合型離心微流體晶片用於全血中無標記癌細胞分離及細胞裂解 =Integrated Centrifugal Microfluidic Chip for Label-Free Cancer Cell Separation and Cell Lysis in Whole Blood /Integrated Centrifugal Microfluidic Chip for Label-Free Cancer Cell Separation and Cell Lysis in Whole Blood.劉怡志. - 初版. - 雲林縣 :國立虎尾科技大學 ,民113.07. - [13], 83面 :圖, 表 ;30公分.

指導教授: 郭如男.

碩士論文--國立虎尾科技大學自動化工程系碩士班.

含參考書目.

循環腫瘤細胞檢測是透過血液採樣後分離計數癌細胞數量，進一步可將癌細胞裂解釋放內部的蛋白質、核酸及代謝物質，具有方便及非侵入性優點成為近年癌症早期檢測利器。本研究設計由血液中分離K562癌細胞並與裂解液進行細胞裂解的整合型微流體晶片，透過收縮-膨脹陣列結構(Contraction-Expansion Array, CEA)與離心平台上的主要四種流體動力效應：離心力、科氏力、慣性升力及迪恩拖曳力進行癌細胞分離。晶片設計虹吸閥門在對應轉速突破的特性對晶片上各步驟的流體進行限制及流通。首先利用COMSOL Multiphysics軟體模擬對三種不同形狀的CEA流道進行流場粒子軌跡的模擬，結果顯示梯形CEA流道的分離效率為最佳，在2250 rpm轉速下時達93.3%分離效率，也透過流場分析流道結構中的各種流體作用力，混合流道模擬結果在2750 rpm轉速下有最高95.9%的混合效率。實驗使用不同血容比條件下固定血球細胞比K562癌細胞比例約107：1進行，使用PBS磷酸鹽緩衝液作為邊鞘流，實驗結果顯示6%血容比在2250 rpm轉速下有最高91.8%的分離效率。而混合流道的實驗使用紅藍色的染料進行，透過Image J影像處理軟體對混合出口槽的液體顏色進行混合效率的計算，得出結果在2750 rpm轉速時有最高混合效率94.8%。在最後的細胞裂解反應槽中，設計了一段凹陷的裂解觀察槽，透過槽旁的虹吸流道將反應完的廢液送至廢液槽，使裂解K562癌細胞成分集中於觀察槽中，結果顯示使用100%濃度的裂解液可在約30秒裂解K562細胞膜。此整合型微流體晶片從樣品注入、細胞分離、混合到癌細胞裂解全程在2分鐘內自動完成。.

(平裝)Subjects--Topical Terms:

997894
COMSOL.

整合型離心微流體晶片用於全血中無標記癌細胞分離及細胞裂解 = = Integrated Centrifugal Microfluidic Chip for Label-Free Cancer Cell Separation and Cell Lysis in Whole Blood /
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245 1 0 $a 整合型離心微流體晶片用於全血中無標記癌細胞分離及細胞裂解 = $b Integrated Centrifugal Microfluidic Chip for Label-Free Cancer Cell Separation and Cell Lysis in Whole Blood / $c 劉怡志.
246 1 1 $a Integrated Centrifugal Microfluidic Chip for Label-Free Cancer Cell Separation and Cell Lysis in Whole Blood.
250 $a 初版.
260 # $a 雲林縣 : $b 國立虎尾科技大學 , $c 民113.07.
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500 $a 學年度: 112.
502 $a 碩士論文--國立虎尾科技大學自動化工程系碩士班.
504 $a 含參考書目.
520 3 $a 循環腫瘤細胞檢測是透過血液採樣後分離計數癌細胞數量，進一步可將癌細胞裂解釋放內部的蛋白質、核酸及代謝物質，具有方便及非侵入性優點成為近年癌症早期檢測利器。本研究設計由血液中分離K562癌細胞並與裂解液進行細胞裂解的整合型微流體晶片，透過收縮-膨脹陣列結構(Contraction-Expansion Array, CEA)與離心平台上的主要四種流體動力效應：離心力、科氏力、慣性升力及迪恩拖曳力進行癌細胞分離。晶片設計虹吸閥門在對應轉速突破的特性對晶片上各步驟的流體進行限制及流通。首先利用COMSOL Multiphysics軟體模擬對三種不同形狀的CEA流道進行流場粒子軌跡的模擬，結果顯示梯形CEA流道的分離效率為最佳，在2250 rpm轉速下時達93.3%分離效率，也透過流場分析流道結構中的各種流體作用力，混合流道模擬結果在2750 rpm轉速下有最高95.9%的混合效率。實驗使用不同血容比條件下固定血球細胞比K562癌細胞比例約107：1進行，使用PBS磷酸鹽緩衝液作為邊鞘流，實驗結果顯示6%血容比在2250 rpm轉速下有最高91.8%的分離效率。而混合流道的實驗使用紅藍色的染料進行，透過Image J影像處理軟體對混合出口槽的液體顏色進行混合效率的計算，得出結果在2750 rpm轉速時有最高混合效率94.8%。在最後的細胞裂解反應槽中，設計了一段凹陷的裂解觀察槽，透過槽旁的虹吸流道將反應完的廢液送至廢液槽，使裂解K562癌細胞成分集中於觀察槽中，結果顯示使用100%濃度的裂解液可在約30秒裂解K562細胞膜。此整合型微流體晶片從樣品注入、細胞分離、混合到癌細胞裂解全程在2分鐘內自動完成。.
520 3 $a Circulating tumor cell detection is to separate and count the number of cancer cells in the sampled blood, and then further lyse the cancer cells to release the protein, nucleic acids and metabolites inside the cancer cells. With the advantages of convenience and non-invasion, it has become a useful method for early detection of cancers in recent years. In this study, an integrated microfluidic chip was designed to separate K562 cancer cells from blood and lyse them with the buffer, that is, cancer cells were separated through the Contraction-Expansion Array (CEA) and four main hydrodynamic effects on the centrifugal platform: centrifugal force, Coriolis force, inertial lift force and Dean drag force. The siphon valve was designed on the chip to restrict and circulate the fluid in each step on the chip depending on the characteristic of corresponding excessive velocity triggering. Firstly, the particle trajectories of three different shapes of CEA channels were simulated by using the COMSOL Multiphysics software. The results showed that the separation efficiency of the trapezoidal CEA channel was the best, reaching 93.3% at 2250 rpm. Through the analysis of various fluid forces in the channel structure in the flow field, the simulation results of the mixing channel showed the highest mixing efficiency, reaching 95.9% at 2750 rpm. In the test, the ratio of fixed blood cells to K562 cancer cells under different blood volume ratios was about 107: 1, and PBS phosphate buffer was used as the edge flow. The test results showed that 6% blood volume ratio had the highest separation efficiency of 91.8% at 2250 rpm. The mixing channel was tested with reddish blue dyes, and the mixing efficiency was calculated depending on the liquid color in the mixing chamber at the outlet by using the Image J image processing software, and the result showed that the highest mixing efficiency was 94.8% at 2750 rpm. A concave lysis observation tank was designed in the last cell lysis reaction tank, and the reacted waste liquid was sent to the waste liquid tank through the siphon channel beside the tank, so that the components of lysed K562 cancer cells were concentrated in the observation tank. The results showed that K562 cytomembrane could be lysed in about 30 seconds with 100% lysis buffer. The integrated microfluidic chip can automatically complete the whole process from sample injection, cell separation and mixing to cancer cell lysis within 2 minutes..
563 $a (平裝)
650 # 4 $a COMSOL. $3 997894
650 # 4 $a Contraction-Expansion Array (CEA). $3 1450051
650 # 4 $a centrifugal microfluidic. $3 1450045
650 # 4 $a label-free cancer cells. $3 1450050
650 # 4 $a 收縮-膨脹陣列結構. $3 1450049
650 # 4 $a 離心微流體. $3 1450043
650 # 4 $a 無標記癌細胞. $3 1450048
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