國立虎尾科技大學 |

Metagenomic Insights into the Full-Scale Anaerobic Digestion of Cow Manure and Pre-treated Food Waste.

Record Type:	Language materials, manuscript : Monograph/item
Title/Author:	Metagenomic Insights into the Full-Scale Anaerobic Digestion of Cow Manure and Pre-treated Food Waste./
Author:	DeCola, Amy.
Description:	1 online resource (78 pages)
Notes:	Source: Masters Abstracts International, Volume: 84-08.
Contained By:	Masters Abstracts International84-08.
Subject:	Environmental engineering. -
Online resource:	click for full text (PQDT)
ISBN:	9798371989918

Metagenomic Insights into the Full-Scale Anaerobic Digestion of Cow Manure and Pre-treated Food Waste.
DeCola, Amy.

Metagenomic Insights into the Full-Scale Anaerobic Digestion of Cow Manure and Pre-treated Food Waste. - 1 online resource (78 pages)

Source: Masters Abstracts International, Volume: 84-08.

Thesis (M.S.)--The University of Vermont and State Agricultural College, 2023.

Includes bibliographical references

Anaerobic digestion provides many benefits, such as diverting organic waste from landfills, promoting nutrient recovery, reducing greenhouse gas emissions, and producing renewable energy. A complex community of microorganisms is responsible for the anaerobic digestion process that converts organic matter into biogas, a renewable energy source. The start-up phase of an anaerobic digester is considered a crucial point in solidifying the core microbial community that will ensure a stable anaerobic digester. A full-scale anaerobic digester that co-digests cow manure and pre-treated food waste from a hydrolysis tank was studied from start-up through stable performance in order to better understand the dynamics of the core microbial community, assess gene abundance, and relate population dynamics to operational parameters.The microbial community in the hydrolysis tank, which was operated at a low pH of 3.5 to 4.4, consisted of organisms known to ferment carbohydrates to acetate and lactate, such as those in the genera Lactobacillus, Prevotella, and Bifidobacterium. Genes for the Bifid shunt were abundant while genes for hydrogen production and ferredoxin production and consumption were absent, suggesting that fermentation likely did not result in H2 production.The methanogenic archaeal community in the anaerobic digester had a high relative abundance up to 25%. The inoculation of the digester had little impact on abundant organisms, as Methanoculleus and an unclassified Methanomicrobiales genera were highly abundant before and after inoculation, though the abundance of total methanogenic archaea decreased after inoculation. While some genes related to acetoclastic methanogenesis were present, two diagnostic genes of this pathway (ack and pta) were absent, suggesting that hydrogenotrophic methanogenesis was the primary route. The high abundance of genes involved in ferredoxin cycling and H2 generation suggest that bacteria in the digester were likely producing H2.

Electronic reproduction.
Ann Arbor, Mich. :
ProQuest,
2024

Mode of access: World Wide Web

ISBN: 9798371989918Subjects--Topical Terms:

557376
Environmental engineering.
Subjects--Index Terms:

Anaerobic digestionIndex Terms--Genre/Form:

554714
Electronic books.

Metagenomic Insights into the Full-Scale Anaerobic Digestion of Cow Manure and Pre-treated Food Waste.
LDR:03337ntm a2200373K 4500 001 1141034
005 20231130111553.5
006 m o d
007 cr mn ---uuuuu
008 250605s2023 xx obm 000 0 eng d
020 $a 9798371989918
035 $a (MiAaPQ)AAI30248302
035 $a AAI30248302
040 $a MiAaPQ $b eng $c MiAaPQ $d NTU
100 1 $a DeCola, Amy. $3 1464601
245 1 0 $a Metagenomic Insights into the Full-Scale Anaerobic Digestion of Cow Manure and Pre-treated Food Waste.
264 0 $c 2023
300 $a 1 online resource (78 pages)
336 $a text $b txt $2 rdacontent
337 $a computer $b c $2 rdamedia
338 $a online resource $b cr $2 rdacarrier
500 $a Source: Masters Abstracts International, Volume: 84-08.
500 $a Advisor: Scarborough, Matthew.
502 $a Thesis (M.S.)--The University of Vermont and State Agricultural College, 2023.
504 $a Includes bibliographical references
520 $a Anaerobic digestion provides many benefits, such as diverting organic waste from landfills, promoting nutrient recovery, reducing greenhouse gas emissions, and producing renewable energy. A complex community of microorganisms is responsible for the anaerobic digestion process that converts organic matter into biogas, a renewable energy source. The start-up phase of an anaerobic digester is considered a crucial point in solidifying the core microbial community that will ensure a stable anaerobic digester. A full-scale anaerobic digester that co-digests cow manure and pre-treated food waste from a hydrolysis tank was studied from start-up through stable performance in order to better understand the dynamics of the core microbial community, assess gene abundance, and relate population dynamics to operational parameters.The microbial community in the hydrolysis tank, which was operated at a low pH of 3.5 to 4.4, consisted of organisms known to ferment carbohydrates to acetate and lactate, such as those in the genera Lactobacillus, Prevotella, and Bifidobacterium. Genes for the Bifid shunt were abundant while genes for hydrogen production and ferredoxin production and consumption were absent, suggesting that fermentation likely did not result in H2 production.The methanogenic archaeal community in the anaerobic digester had a high relative abundance up to 25%. The inoculation of the digester had little impact on abundant organisms, as Methanoculleus and an unclassified Methanomicrobiales genera were highly abundant before and after inoculation, though the abundance of total methanogenic archaea decreased after inoculation. While some genes related to acetoclastic methanogenesis were present, two diagnostic genes of this pathway (ack and pta) were absent, suggesting that hydrogenotrophic methanogenesis was the primary route. The high abundance of genes involved in ferredoxin cycling and H2 generation suggest that bacteria in the digester were likely producing H2.
533 $a Electronic reproduction. $b Ann Arbor, Mich. : $c ProQuest, $d 2024
538 $a Mode of access: World Wide Web
650 4 $a Environmental engineering. $3 557376
650 4 $a Microbiology. $3 591510
653 $a Anaerobic digestion
653 $a Cow manure
653 $a Food waste
653 $a Metagenomics
655 7 $a Electronic books. $2 local $3 554714
690 $a 0775
690 $a 0410
710 2 $a ProQuest Information and Learning Co. $3 1178819
710 2 $a The University of Vermont and State Agricultural College. $b Civil and Environmental Engineering. $3 1464602
773 0 $t Masters Abstracts International $g 84-08.
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=30248302 $z click for full text (PQDT)