國立虎尾科技大學 |

Circular Plastics Economies for Mars and Beyond : = 3D Printing a Sustainable Future.

紀錄類型:	書目-語言資料,手稿 : Monograph/item
正題名/作者:	Circular Plastics Economies for Mars and Beyond :/
其他題名:	3D Printing a Sustainable Future.
作者:	Pane, Vincent.
面頁冊數:	1 online resource (304 pages)
附註:	Source: Dissertations Abstracts International, Volume: 85-04, Section: B.
Contained By:	Dissertations Abstracts International85-04B.
標題:	Polymers. -
電子資源:	click for full text (PQDT)
ISBN:	9798380485920

Circular Plastics Economies for Mars and Beyond : = 3D Printing a Sustainable Future.
Pane, Vincent.

Circular Plastics Economies for Mars and Beyond :3D Printing a Sustainable Future. - 1 online resource (304 pages)

Source: Dissertations Abstracts International, Volume: 85-04, Section: B.

Thesis (Ph.D.)--Stanford University, 2023.

Includes bibliographical references

My graduate research explored the synthesis, characterization, and applications of synthetically- and microbially-derived biodegradable polymers. The broad scope of my work was motivated through the NASA institute, CUBES (Center for the Utilization of Biological Engineering in Space). The goal of this group was to use the tools of biological engineering to create a proof-of-concept architecture for a manned mission to Mars. Out of the four interconnected branches that comprised CUBES, my research was part of Biofuel and Biomanufacturing Division (BBMD). This group focused on tool manufacturing, and as such, our goal was to derive polymers through microbial engineering and use them to manufacture useful mission parts via 3D printing.Poly-3-hydroxybutyrate (P3HB) stood out as the most amenable polymer for our purpose, since it is readily available microbially; however, P3HB, has a high crystallinity and a low degradation temperature, making its processing window for manufacturing quite limited. Fused Filament Fabrication (FFF) proved to be the most viable manufacturing option given these constraints, but FFF was still poorly suited for 3D printing P3HB. I engineered solutions for this by using careful temperature control to create uniform printer filament and by altering the print bed surface to control part warping and prevent delamination. Though mission-relevant parts could now be successfully printed using FFF, we were not able to overcome low tolerances on filament production which ultimately constrained the largest printable parts to a few grams.The limitations of 3D printing P3HB helped motivate much of the microbial work of the BBMD in CUBES. Methanotrophs and other microbes rely on sources of CO2, CH4, and O2 for both cell growth and energy storage as P3HB. Understanding the mechanisms by which PHAs (Polyhydroxyalkanoates- a category of polyesters including P3HB) are synthesized microbially can provide valuable insight into how to both maximize PHA accumulation and alter polymer composition. Atmospheric contents for common methanotroph organisms OB3B and OBPB were carefully controlled and monitored under biomass and PHA accumulation conditions to determine the fate of each feedstock gas. Microbes were then fed 13C-labeled CO2 and CH4 to trace their uptake into P3HB and thereby elucidate metabolic features. By 13C NMR, we showed that CO2 contributed to the more oxidized C1 and C3 carbons of the P3HB repeat while the CH4 contributed to the more reduced C2 and C4 carbons. This provided strong support for CH4 incorporation as formate in the serine cycle and CO2utilization in the biosynthesis of oxaloacetate to ultimately form P3HB.With knowledge of the metabolic pathways for P3HB biosynthesis, microbes could be engineered to take up novel substrates. By incorporation of various short-chain fatty acids into the polymer, P3HB's high crystallinity, low elasticity, and narrow processing window are known to be tunable. To broaden the scope of our control over the bio-PHA material properties, Cupriavidus necatorH16 was engineered to uptake novel aryl substrates into the PHA backbone by means of heterologous hydroxyacyl-CoA transferase and mutant PHA synthase. Through NMR studies we showed the first formation of biological polyesters incorporating aromatic rings in the backbone (hydroxyphenylic and a hydroxyfuranoic acid).

Electronic reproduction.
Ann Arbor, Mich. :
ProQuest,
2024

Mode of access: World Wide Web

ISBN: 9798380485920Subjects--Topical Terms:

561233
Polymers.
Index Terms--Genre/Form:

554714
Electronic books.

Circular Plastics Economies for Mars and Beyond : = 3D Printing a Sustainable Future.
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