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Exploring Deep Learning Methods for Low Numerical Aperture to High Numerical Aperture Resolution Enhancement in Confocal Microscopy.

紀錄類型:	書目-語言資料,手稿 : Monograph/item
正題名/作者:	Exploring Deep Learning Methods for Low Numerical Aperture to High Numerical Aperture Resolution Enhancement in Confocal Microscopy./
作者:	Patra, Shashwat Rajan.
面頁冊數:	1 online resource (67 pages)
附註:	Source: Masters Abstracts International, Volume: 84-10.
Contained By:	Masters Abstracts International84-10.
標題:	Computer engineering. -
電子資源:	click for full text (PQDT)
ISBN:	9798379403379

Exploring Deep Learning Methods for Low Numerical Aperture to High Numerical Aperture Resolution Enhancement in Confocal Microscopy.
Patra, Shashwat Rajan.

Exploring Deep Learning Methods for Low Numerical Aperture to High Numerical Aperture Resolution Enhancement in Confocal Microscopy. - 1 online resource (67 pages)

Source: Masters Abstracts International, Volume: 84-10.

Thesis (M.S.)--The University of Memphis, 2023.

Includes bibliographical references

Confocal microscopy is a widely used tool that provides valuable morphological and functional information within cells and tissues. A major advantage of confocal microscopy is its ability to record multi-color and optically sectioned images. A major drawback to confocal microscopy is its diffraction-limited spatial resolution. Though techniques have been developed that break this limit in confocal microscopy, they require additional hardware or accurate estimates of the system's impulse response (e.g., point spread function). Here we investigate two deep learning-based models, the cGAN and cycleGAN, trained with low-resolution (LR) and high-resolution (HR) confocal images to improve spatial resolution in confocal microscopy. Our findings conclude that the cGAN can accurately produce HR images if the training set contains images with a high signal-to-noise ratio. We have also found that the cycleGAN model has the potential to perform as the cGAN model but without the requirement of using paired inputs.

Electronic reproduction.
Ann Arbor, Mich. :
ProQuest,
2024

Mode of access: World Wide Web

ISBN: 9798379403379Subjects--Topical Terms:

569006
Computer engineering.
Subjects--Index Terms:

Deep learning methodsIndex Terms--Genre/Form:

554714
Electronic books.

Exploring Deep Learning Methods for Low Numerical Aperture to High Numerical Aperture Resolution Enhancement in Confocal Microscopy.
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520 $a Confocal microscopy is a widely used tool that provides valuable morphological and functional information within cells and tissues. A major advantage of confocal microscopy is its ability to record multi-color and optically sectioned images. A major drawback to confocal microscopy is its diffraction-limited spatial resolution. Though techniques have been developed that break this limit in confocal microscopy, they require additional hardware or accurate estimates of the system's impulse response (e.g., point spread function). Here we investigate two deep learning-based models, the cGAN and cycleGAN, trained with low-resolution (LR) and high-resolution (HR) confocal images to improve spatial resolution in confocal microscopy. Our findings conclude that the cGAN can accurately produce HR images if the training set contains images with a high signal-to-noise ratio. We have also found that the cycleGAN model has the potential to perform as the cGAN model but without the requirement of using paired inputs.
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653 $a High numerical aperture
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653 $a Optically sectioned images
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