國立虎尾科技大學 |

The Role of Glutamate in the Medial Prefrontal Cortex in the Acquisition and Expression of Conditioned Approach.

紀錄類型:	書目-語言資料,手稿 : Monograph/item
正題名/作者:	The Role of Glutamate in the Medial Prefrontal Cortex in the Acquisition and Expression of Conditioned Approach./
作者:	Nisanov, Rudolf.
面頁冊數:	1 online resource (130 pages)
附註:	Source: Dissertations Abstracts International, Volume: 85-07, Section: B.
Contained By:	Dissertations Abstracts International85-07B.
標題:	Neurosciences. -
電子資源:	click for full text (PQDT)
ISBN:	9798381429169

The Role of Glutamate in the Medial Prefrontal Cortex in the Acquisition and Expression of Conditioned Approach.
Nisanov, Rudolf.

The Role of Glutamate in the Medial Prefrontal Cortex in the Acquisition and Expression of Conditioned Approach. - 1 online resource (130 pages)

Source: Dissertations Abstracts International, Volume: 85-07, Section: B.

Thesis (Ph.D.)--City University of New York, 2024.

Includes bibliographical references

A series of experiments were conducted to assess the role of glutamatergic stimulation in the dorsal and ventral regions of the medial prefrontal cortex (mPFC) in both the acquisition and expression of reward-related learning using a Pavlovian conditioning paradigm, the conditioned-approach paradigm. Rats with surgically implanted cannulas were exposed to Pavlovian conditioning sessions that occurred on three alternative days (acquisition) or seven consecutive days (expression). These 60-min conditioning sessions consisted of 30 pairings of light (CS) and food pellets (US) presented under a random time schedule. After a two-day break, rats underwent a session with no CS or US and a CS-only (extinction) test session on the following day. For the acquisition of conditioned approach, NMDA receptors were blocked with AP5 prior to each of the three conditioning sessions. For expression, AMPA receptors were blocked with CNQX prior to the CS-only test session. The results from the acquisition experiments show substantially greater responding for the CS (conditioned approach) in rats with inhibited NMDA receptors in both regions of mPFC in comparison to control groups. In the expression experiments, however, inhibition of AMPA receptors in the dorsal region resulted in significant dose-dependent impairment of conditioned approach but in the ventral region it resulted in dose-dependent facilitation of conditioned approach in comparison to the respective control groups. An RNAscope was employed to assess whether the CS - a food-paired light stimulus -activated more neurons than the light stimulus not explicitly paired with food (neutral stimulus). The results revealed that the CS resulted in significantly higher neuronal activation than the non-CS and that the neuronal population that was affected were glutamatergic neurons. The final experiment used chemogenetics to inhibit glutamatergic projections from dmPFC to NAc, finding a significant impairment in conditioned approach expression. Confocal microscopy revealed the pathway originating from dmPFC glutamatergic cells to NAc, supporting the role of top-down executive control in learned behavior expression.

Electronic reproduction.
Ann Arbor, Mich. :
ProQuest,
2024

Mode of access: World Wide Web

ISBN: 9798381429169Subjects--Topical Terms:

593561
Neurosciences.
Subjects--Index Terms:

Conditioned approachIndex Terms--Genre/Form:

554714
Electronic books.

The Role of Glutamate in the Medial Prefrontal Cortex in the Acquisition and Expression of Conditioned Approach.
LDR:03601ntm a22003977 4500 001 1144677
005 20240611104335.5
006 m o d
007 cr mn ---uuuuu
008 250605s2024 xx obm 000 0 eng d
020 $a 9798381429169
035 $a (MiAaPQ)AAI30989082
035 $a AAI30989082
040 $a MiAaPQ $b eng $c MiAaPQ $d NTU
100 1 $a Nisanov, Rudolf. $3 1469790
245 1 4 $a The Role of Glutamate in the Medial Prefrontal Cortex in the Acquisition and Expression of Conditioned Approach.
264 0 $c 2024
300 $a 1 online resource (130 pages)
336 $a text $b txt $2 rdacontent
337 $a computer $b c $2 rdamedia
338 $a online resource $b cr $2 rdacarrier
500 $a Source: Dissertations Abstracts International, Volume: 85-07, Section: B.
500 $a Advisor: Ranaldi, Robert.
502 $a Thesis (Ph.D.)--City University of New York, 2024.
504 $a Includes bibliographical references
520 $a A series of experiments were conducted to assess the role of glutamatergic stimulation in the dorsal and ventral regions of the medial prefrontal cortex (mPFC) in both the acquisition and expression of reward-related learning using a Pavlovian conditioning paradigm, the conditioned-approach paradigm. Rats with surgically implanted cannulas were exposed to Pavlovian conditioning sessions that occurred on three alternative days (acquisition) or seven consecutive days (expression). These 60-min conditioning sessions consisted of 30 pairings of light (CS) and food pellets (US) presented under a random time schedule. After a two-day break, rats underwent a session with no CS or US and a CS-only (extinction) test session on the following day. For the acquisition of conditioned approach, NMDA receptors were blocked with AP5 prior to each of the three conditioning sessions. For expression, AMPA receptors were blocked with CNQX prior to the CS-only test session. The results from the acquisition experiments show substantially greater responding for the CS (conditioned approach) in rats with inhibited NMDA receptors in both regions of mPFC in comparison to control groups. In the expression experiments, however, inhibition of AMPA receptors in the dorsal region resulted in significant dose-dependent impairment of conditioned approach but in the ventral region it resulted in dose-dependent facilitation of conditioned approach in comparison to the respective control groups. An RNAscope was employed to assess whether the CS - a food-paired light stimulus -activated more neurons than the light stimulus not explicitly paired with food (neutral stimulus). The results revealed that the CS resulted in significantly higher neuronal activation than the non-CS and that the neuronal population that was affected were glutamatergic neurons. The final experiment used chemogenetics to inhibit glutamatergic projections from dmPFC to NAc, finding a significant impairment in conditioned approach expression. Confocal microscopy revealed the pathway originating from dmPFC glutamatergic cells to NAc, supporting the role of top-down executive control in learned behavior expression.
533 $a Electronic reproduction. $b Ann Arbor, Mich. : $c ProQuest, $d 2024
538 $a Mode of access: World Wide Web
650 4 $a Neurosciences. $3 593561
650 4 $a Behavioral psychology. $3 1179418
650 4 $a Behavioral sciences. $3 1148596
653 $a Conditioned approach
653 $a Glutamate
653 $a Learning
653 $a Prefrontal cortex
653 $a Glutamatergic stimulation
655 7 $a Electronic books. $2 local $3 554714
690 $a 0602
690 $a 0384
690 $a 0317
710 2 $a City University of New York. $b Psychology. $3 1185881
710 2 $a ProQuest Information and Learning Co. $3 1178819
773 0 $t Dissertations Abstracts International $g 85-07B.
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=30989082 $z click for full text (PQDT)