國立虎尾科技大學 |

Biofilm Formation on Surfaces Used for Food Processing Under Varying Hydrodynamic Shear Stresses and Biofilm Removal Using Mechanical and Chemical Stressors.

Record Type:	Language materials, manuscript : Monograph/item
Title/Author:	Biofilm Formation on Surfaces Used for Food Processing Under Varying Hydrodynamic Shear Stresses and Biofilm Removal Using Mechanical and Chemical Stressors./
Author:	Prabhukhot, Grishma.
Description:	1 online resource (219 pages)
Notes:	Source: Dissertations Abstracts International, Volume: 85-11, Section: B.
Contained By:	Dissertations Abstracts International85-11B.
Subject:	Food science. -
Online resource:	click for full text (PQDT)
ISBN:	9798382744476

Biofilm Formation on Surfaces Used for Food Processing Under Varying Hydrodynamic Shear Stresses and Biofilm Removal Using Mechanical and Chemical Stressors.
Prabhukhot, Grishma.

Biofilm Formation on Surfaces Used for Food Processing Under Varying Hydrodynamic Shear Stresses and Biofilm Removal Using Mechanical and Chemical Stressors. - 1 online resource (219 pages)

Source: Dissertations Abstracts International, Volume: 85-11, Section: B.

Thesis (Ph.D.)--University of Maryland, Baltimore County, 2024.

Includes bibliographical references

Biofilms formed by pathogenic bacteria are complex communities adhering to surfaces, exhibiting increased resistance to disinfectants, thus leading to serious infections. Chemical disinfectants have long been utilized to eliminate pathogenic biofilms. Despite their potency and reliability, preliminary research suggests chemical disinfectants do not consistently achieve complete biofilm eradication. This PhD dissertation research focuses on investigating the factors governing biofilm formation and removal from food contact surfaces, such as identifying whether eithersurface roughness, kurtosis, or skewness promotes biofilm formation or if any of these factors provide additional protection against chemical disinfectants.Three research topics are included in this dissertation. The first topic is based on biofilm formation of pathogenic bacteria E. coli O157:H7, L. monocytogenes as a single species biofilm and in the presence of a non-pathogenic promoter bacterium R. insidiosa in a Centers for Disease Control and Prevention (CDC) bioreactor. Materials commonly used for food processing such as SS 316L, PTFE, EPDM, and Polycarbonate are used to grow biofilms at different shear stresses. The results from this study indicate that when E. coli O157:H7 biofilms are formed at low shear stress of 0.368 N/m2, the development is significantly heightened on surfaces with high kurtosis. This study underlines the importance of commonly sidelined surface parameters such as kurtosis. Our second research topic focuses on biofilm removal ability using chlorine from SS 316L, PTFE, and EPDM at a concentration up to 500 ppm for an exposure of 1 and 4 minutes at a flow of 1.2 liters per minute. Post-chlorine treatment, we observed that lower bacterial populations from L. monocytogenes biofilms were present in multispecies environment on PTFE material (high surface roughness), compared to SS 316L (low surface roughness) when these biofilms were grown at high shear stress of 2.462 N/m2. The third topic of this dissertation focuses on the biofilm removal using a more environmentally friendly chemical disinfectant Peroxyacetic acid to remove biofilms. When E. coli O157:H7 biofilms were formed in a multispecies environment at high shear stress and treated with peroxyacetic acid, removing biofilms from EPDM was found to be more challenging.

Electronic reproduction.
Ann Arbor, Mich. :
ProQuest,
2024

Mode of access: World Wide Web

ISBN: 9798382744476Subjects--Topical Terms:

1179759
Food science.
Subjects--Index Terms:

BiofilmsIndex Terms--Genre/Form:

554714
Electronic books.

Biofilm Formation on Surfaces Used for Food Processing Under Varying Hydrodynamic Shear Stresses and Biofilm Removal Using Mechanical and Chemical Stressors.
LDR:03855ntm a22004097 4500 001 1145006
005 20240617111738.5
006 m o d
007 cr mn ---uuuuu
008 250605s2024 xx obm 000 0 eng d
020 $a 9798382744476
035 $a (MiAaPQ)AAI31242828
035 $a AAI31242828
040 $a MiAaPQ $b eng $c MiAaPQ $d NTU
100 1 $a Prabhukhot, Grishma. $3 1470204
245 1 0 $a Biofilm Formation on Surfaces Used for Food Processing Under Varying Hydrodynamic Shear Stresses and Biofilm Removal Using Mechanical and Chemical Stressors.
264 0 $c 2024
300 $a 1 online resource (219 pages)
336 $a text $b txt $2 rdacontent
337 $a computer $b c $2 rdamedia
338 $a online resource $b cr $2 rdacarrier
500 $a Source: Dissertations Abstracts International, Volume: 85-11, Section: B.
500 $a Advisor: Eggleton, Charles D.;Patel, Jitendra.
502 $a Thesis (Ph.D.)--University of Maryland, Baltimore County, 2024.
504 $a Includes bibliographical references
520 $a Biofilms formed by pathogenic bacteria are complex communities adhering to surfaces, exhibiting increased resistance to disinfectants, thus leading to serious infections. Chemical disinfectants have long been utilized to eliminate pathogenic biofilms. Despite their potency and reliability, preliminary research suggests chemical disinfectants do not consistently achieve complete biofilm eradication. This PhD dissertation research focuses on investigating the factors governing biofilm formation and removal from food contact surfaces, such as identifying whether eithersurface roughness, kurtosis, or skewness promotes biofilm formation or if any of these factors provide additional protection against chemical disinfectants.Three research topics are included in this dissertation. The first topic is based on biofilm formation of pathogenic bacteria E. coli O157:H7, L. monocytogenes as a single species biofilm and in the presence of a non-pathogenic promoter bacterium R. insidiosa in a Centers for Disease Control and Prevention (CDC) bioreactor. Materials commonly used for food processing such as SS 316L, PTFE, EPDM, and Polycarbonate are used to grow biofilms at different shear stresses. The results from this study indicate that when E. coli O157:H7 biofilms are formed at low shear stress of 0.368 N/m2, the development is significantly heightened on surfaces with high kurtosis. This study underlines the importance of commonly sidelined surface parameters such as kurtosis. Our second research topic focuses on biofilm removal ability using chlorine from SS 316L, PTFE, and EPDM at a concentration up to 500 ppm for an exposure of 1 and 4 minutes at a flow of 1.2 liters per minute. Post-chlorine treatment, we observed that lower bacterial populations from L. monocytogenes biofilms were present in multispecies environment on PTFE material (high surface roughness), compared to SS 316L (low surface roughness) when these biofilms were grown at high shear stress of 2.462 N/m2. The third topic of this dissertation focuses on the biofilm removal using a more environmentally friendly chemical disinfectant Peroxyacetic acid to remove biofilms. When E. coli O157:H7 biofilms were formed in a multispecies environment at high shear stress and treated with peroxyacetic acid, removing biofilms from EPDM was found to be more challenging.
533 $a Electronic reproduction. $b Ann Arbor, Mich. : $c ProQuest, $d 2024
538 $a Mode of access: World Wide Web
650 4 $a Food science. $3 1179759
650 4 $a Biomechanics. $3 565307
650 4 $a Agricultural engineering. $3 1148660
653 $a Biofilms
653 $a Bioreactor
653 $a E. coli O157:H7
653 $a Food safety
653 $a L. monocytogenes
653 $a Shear stress
655 7 $a Electronic books. $2 local $3 554714
690 $a 0359
690 $a 0539
690 $a 0648
710 2 $a ProQuest Information and Learning Co. $3 1178819
710 2 $a University of Maryland, Baltimore County. $b Engineering, Mechanical. $3 1178896
773 0 $t Dissertations Abstracts International $g 85-11B.
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=31242828 $z click for full text (PQDT)