國立虎尾科技大學 |

Conservation genetics of California abalone species.

Record Type:	Language materials, manuscript : Monograph/item
Title/Author:	Conservation genetics of California abalone species./
Author:	Gruenthal, Kristen Marie.
Description:	1 online resource (249 pages)
Notes:	Source: Dissertations Abstracts International, Volume: 69-03, Section: B.
Contained By:	Dissertations Abstracts International69-03B.
Subject:	Genetics. -
Online resource:	click for full text (PQDT)
ISBN:	9780549075981

Conservation genetics of California abalone species.
Gruenthal, Kristen Marie.

Conservation genetics of California abalone species. - 1 online resource (249 pages)

Source: Dissertations Abstracts International, Volume: 69-03, Section: B.

Thesis (Ph.D.)--University of California, San Diego, 2007.

Includes bibliographical references

Over the past three decades, five species of abalone (genus Haliotis) in California waters have gone from harvestable resources to rare or even endangered species status. Both anthropogenic (overexploitation, habitat destruction) and natural (disease, predation) factors have contributed to the declines. This dissertation explores several aspects of abalone recovery and conservation along the coast of California through population genetic theory and techniques. Chapters I and II deal with the captive breeding of two California abalone species. Chapter I focuses on ensuring the specific purity of broodstock and maintaining genetic diversity in hatchery-bred progeny of the endangered white abalone (H. soreseni). Using DNA from these animals, genetic markers were developed, including five nuclear microsatellite loci and partial sequences of one nuclear (VERL) and two mitochondrial (COI and CytB) genes, to assess genetic variability in the species, aid in species identification (ergo prevent broodstock contamination), and potentially track the success of future outplanting of captive-reared animals in restocking operations. Chapter II quantifies the loss of genetic diversity resulting from the captive breeding of green abalone (H. fulgens). No change in overall heterozygosity was evident, but significant losses in allelic richness were found in the captive-bred green abalone versus that in wild populations. Chapters III, IV, and V center on inferring realized connectivity (gene flow) among natural populations of red (H. rufescens ), black (H. cracherodii), and pink (H. corrugata) abalone. For red abalone (Chapter III), COI sequencing and microsatellite genotyping did not show significant genetic divergence among populations. In contrast, data from AFLPs became the first to suggest there is significant genetic differentiation among California red abalone populations. In black abalone (Chapter IV), data from AFLPs and one microsatellite locus showed significant divergence among multiple populations and exhibited a signal of isolation by distance consistent with a stepping-stone model of connectivity. Pink abalone (Chapter V) also showed evidence of restricted gene flow among populations. Finally, Chapters VI and VII focus on the fatal disease withering syndrome (WS). Chapter VI involves characterizing the post-esophageal microbiomes of red and black abalone with and without WS. Significant differences between the clone libraries isolated from healthy and diseased abalone suggested that WS has significant impact on the bacterial composition of the abalone post-esophageal microbiome, and specific membership of the microbiomes suggests that infection by Candidatus Xenohaliotis californiensis, the etiological agent of WS, may not be the sole cause of morbidity and mortality due to WS in abalone. Chapter VII is a preliminary study of genetic variation within C. X. californiensis samples from wild and captive-bred abalone species collected from multiple locations along the coast of California. Thus far, no variation has been found in partial sequences of either the 16S rDNA or recombinase A (recA) genes.

Electronic reproduction.
Ann Arbor, Mich. :
ProQuest,
2024

Mode of access: World Wide Web

ISBN: 9780549075981Subjects--Topical Terms:

578972
Genetics.
Subjects--Index Terms:

AbaloneIndex Terms--Genre/Form:

554714
Electronic books.

Conservation genetics of California abalone species.
LDR:04526ntm a22003977 4500 001 1145024
005 20240617111742.5
006 m o d
007 cr mn ---uuuuu
008 250605s2007 xx obm 000 0 eng d
020 $a 9780549075981
035 $a (MiAaPQ)AAI3268587
035 $a (MiAaPQ)ucsd:1684
035 $a AAI3268587
040 $a MiAaPQ $b eng $c MiAaPQ $d NTU
100 1 $a Gruenthal, Kristen Marie. $3 1470226
245 1 0 $a Conservation genetics of California abalone species.
264 0 $c 2007
300 $a 1 online resource (249 pages)
336 $a text $b txt $2 rdacontent
337 $a computer $b c $2 rdamedia
338 $a online resource $b cr $2 rdacarrier
500 $a Source: Dissertations Abstracts International, Volume: 69-03, Section: B.
500 $a Publisher info.: Dissertation/Thesis.
500 $a Advisor: Burton, Ronald S.
502 $a Thesis (Ph.D.)--University of California, San Diego, 2007.
504 $a Includes bibliographical references
520 $a Over the past three decades, five species of abalone (genus Haliotis) in California waters have gone from harvestable resources to rare or even endangered species status. Both anthropogenic (overexploitation, habitat destruction) and natural (disease, predation) factors have contributed to the declines. This dissertation explores several aspects of abalone recovery and conservation along the coast of California through population genetic theory and techniques. Chapters I and II deal with the captive breeding of two California abalone species. Chapter I focuses on ensuring the specific purity of broodstock and maintaining genetic diversity in hatchery-bred progeny of the endangered white abalone (H. soreseni). Using DNA from these animals, genetic markers were developed, including five nuclear microsatellite loci and partial sequences of one nuclear (VERL) and two mitochondrial (COI and CytB) genes, to assess genetic variability in the species, aid in species identification (ergo prevent broodstock contamination), and potentially track the success of future outplanting of captive-reared animals in restocking operations. Chapter II quantifies the loss of genetic diversity resulting from the captive breeding of green abalone (H. fulgens). No change in overall heterozygosity was evident, but significant losses in allelic richness were found in the captive-bred green abalone versus that in wild populations. Chapters III, IV, and V center on inferring realized connectivity (gene flow) among natural populations of red (H. rufescens ), black (H. cracherodii), and pink (H. corrugata) abalone. For red abalone (Chapter III), COI sequencing and microsatellite genotyping did not show significant genetic divergence among populations. In contrast, data from AFLPs became the first to suggest there is significant genetic differentiation among California red abalone populations. In black abalone (Chapter IV), data from AFLPs and one microsatellite locus showed significant divergence among multiple populations and exhibited a signal of isolation by distance consistent with a stepping-stone model of connectivity. Pink abalone (Chapter V) also showed evidence of restricted gene flow among populations. Finally, Chapters VI and VII focus on the fatal disease withering syndrome (WS). Chapter VI involves characterizing the post-esophageal microbiomes of red and black abalone with and without WS. Significant differences between the clone libraries isolated from healthy and diseased abalone suggested that WS has significant impact on the bacterial composition of the abalone post-esophageal microbiome, and specific membership of the microbiomes suggests that infection by Candidatus Xenohaliotis californiensis, the etiological agent of WS, may not be the sole cause of morbidity and mortality due to WS in abalone. Chapter VII is a preliminary study of genetic variation within C. X. californiensis samples from wild and captive-bred abalone species collected from multiple locations along the coast of California. Thus far, no variation has been found in partial sequences of either the 16S rDNA or recombinase A (recA) genes.
533 $a Electronic reproduction. $b Ann Arbor, Mich. : $c ProQuest, $d 2024
538 $a Mode of access: World Wide Web
650 4 $a Genetics. $3 578972
650 4 $a Oceanography. $3 654897
650 4 $a Biological oceanography. $3 1178855
653 $a Abalone
653 $a California
653 $a Conservation genetics
653 $a Haliotis
655 7 $a Electronic books. $2 local $3 554714
690 $a 0369
690 $a 0416
710 2 $a ProQuest Information and Learning Co. $3 1178819
710 2 $a University of California, San Diego. $b Marine Biology. $3 1470227
773 0 $t Dissertations Abstracts International $g 69-03B.
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3268587 $z click for full text (PQDT)