國立虎尾科技大學 |

Development of Rapid and Portable Detection Methods for Antibiotics, Viruses, and Antibiotic-Resistant Bacteria in Wastewater.

Record Type:	Language materials, manuscript : Monograph/item
Title/Author:	Development of Rapid and Portable Detection Methods for Antibiotics, Viruses, and Antibiotic-Resistant Bacteria in Wastewater./
Author:	Huang, Yen-Hsiang.
Description:	1 online resource (124 pages)
Notes:	Source: Dissertations Abstracts International, Volume: 85-11, Section: B.
Contained By:	Dissertations Abstracts International85-11B.
Subject:	Environmental engineering. -
Online resource:	click for full text (PQDT)
ISBN:	9798382733593

Development of Rapid and Portable Detection Methods for Antibiotics, Viruses, and Antibiotic-Resistant Bacteria in Wastewater.
Huang, Yen-Hsiang.

Development of Rapid and Portable Detection Methods for Antibiotics, Viruses, and Antibiotic-Resistant Bacteria in Wastewater. - 1 online resource (124 pages)

Source: Dissertations Abstracts International, Volume: 85-11, Section: B.

Thesis (Ph.D.)--University of California, Irvine, 2024.

Includes bibliographical references

Antibiotic resistance has emerged as a critical global health threat in the 21st century, leading to untreatable infectious diseases and significantly increasing human morbidity and mortality. Rapid, portable, and user-friendly detection of antibiotics, microbial pathogens, and antibiotic-resistant organisms in aquatic environment plays a key role in controlling the spread of diseases and combating the emergence of antibiotic resistance. Unfortunately, current environmental monitoring practices are tedious, expensive and slow. These limitations impede timely detection and response to emerging threats, allowing the spread of diseases and antibiotic-resistant pathogens to human populations. This dissertation research aims to develop environmental monitoring strategies that streamline the current monitoring timeline and make it accessible to a wider range of users. This effort will contribute to more frequent and efficient monitoring of antibiotics, microbial pathogens, and antibiotic-resistant organisms in water matrices. Through this research, I first investigated the application of Surface Enhanced Raman Scattering (SERS) for label-free monitoring of antibiotics in wastewater. By utilizing carefully designed SERS substrates and artificial intelligence (AI) algorithms, I successfully quantified the target antibiotics even in the presence of other organic and inorganic molecules in wastewater. Next, I developed a portable and semi-automatic virus detection centrifugal microfluidic disc (CD) that integrates sample concentration, purification, amplification, and quantification steps for environmental water monitoring. The on-CD assay is completed in less than 1.5 hours, and its performance is comparable to that of the benchtop in-tube assay. Lastly, building upon the foundation of the virus detection CD system, I further designed a microfluidic CD that integrates the phenotypic bacterial culture with genotypic pathogen identification for monitoring of antibiotic resistant bacteria. This CD system successfully detected indigenous antibiotic-resistant bacteria in raw wastewater in under 3 hours. This dissertation serves as an important foundation for developing more advanced, field-applicable techniques and methodologies in environmental monitoring.

Electronic reproduction.
Ann Arbor, Mich. :
ProQuest,
2024

Mode of access: World Wide Web

ISBN: 9798382733593Subjects--Topical Terms:

557376
Environmental engineering.
Subjects--Index Terms:

AntibioticsIndex Terms--Genre/Form:

554714
Electronic books.

Development of Rapid and Portable Detection Methods for Antibiotics, Viruses, and Antibiotic-Resistant Bacteria in Wastewater.
LDR:03762ntm a22004217 4500 001 1146269
005 20240812064357.5
006 m o d
007 cr bn ---uuuuu
008 250605s2024 xx obm 000 0 eng d
020 $a 9798382733593
035 $a (MiAaPQ)AAI30996422
035 $a AAI30996422
040 $a MiAaPQ $b eng $c MiAaPQ $d NTU
100 1 $a Huang, Yen-Hsiang. $3 1471629
245 1 0 $a Development of Rapid and Portable Detection Methods for Antibiotics, Viruses, and Antibiotic-Resistant Bacteria in Wastewater.
264 0 $c 2024
300 $a 1 online resource (124 pages)
336 $a text $b txt $2 rdacontent
337 $a computer $b c $2 rdamedia
338 $a online resource $b cr $2 rdacarrier
500 $a Source: Dissertations Abstracts International, Volume: 85-11, Section: B.
500 $a Advisor: Jiang, Sunny.
502 $a Thesis (Ph.D.)--University of California, Irvine, 2024.
504 $a Includes bibliographical references
520 $a Antibiotic resistance has emerged as a critical global health threat in the 21st century, leading to untreatable infectious diseases and significantly increasing human morbidity and mortality. Rapid, portable, and user-friendly detection of antibiotics, microbial pathogens, and antibiotic-resistant organisms in aquatic environment plays a key role in controlling the spread of diseases and combating the emergence of antibiotic resistance. Unfortunately, current environmental monitoring practices are tedious, expensive and slow. These limitations impede timely detection and response to emerging threats, allowing the spread of diseases and antibiotic-resistant pathogens to human populations. This dissertation research aims to develop environmental monitoring strategies that streamline the current monitoring timeline and make it accessible to a wider range of users. This effort will contribute to more frequent and efficient monitoring of antibiotics, microbial pathogens, and antibiotic-resistant organisms in water matrices. Through this research, I first investigated the application of Surface Enhanced Raman Scattering (SERS) for label-free monitoring of antibiotics in wastewater. By utilizing carefully designed SERS substrates and artificial intelligence (AI) algorithms, I successfully quantified the target antibiotics even in the presence of other organic and inorganic molecules in wastewater. Next, I developed a portable and semi-automatic virus detection centrifugal microfluidic disc (CD) that integrates sample concentration, purification, amplification, and quantification steps for environmental water monitoring. The on-CD assay is completed in less than 1.5 hours, and its performance is comparable to that of the benchtop in-tube assay. Lastly, building upon the foundation of the virus detection CD system, I further designed a microfluidic CD that integrates the phenotypic bacterial culture with genotypic pathogen identification for monitoring of antibiotic resistant bacteria. This CD system successfully detected indigenous antibiotic-resistant bacteria in raw wastewater in under 3 hours. This dissertation serves as an important foundation for developing more advanced, field-applicable techniques and methodologies in environmental monitoring.
533 $a Electronic reproduction. $b Ann Arbor, Mich. : $c ProQuest, $d 2024
538 $a Mode of access: World Wide Web
650 4 $a Environmental engineering. $3 557376
650 4 $a Public health. $3 560998
650 4 $a Virology. $3 644995
650 4 $a Water resources management. $3 1178858
653 $a Antibiotics
653 $a Antibiotic-resistant bacteria
653 $a Centrifugal microfluidics
653 $a SERS
653 $a Virus detection
655 7 $a Electronic books. $2 local $3 554714
690 $a 0775
690 $a 0720
690 $a 0800
690 $a 0573
690 $a 0595
710 2 $a ProQuest Information and Learning Co. $3 1178819
710 2 $a University of California, Irvine. $b Civil and Environmental Engineering. $3 1465463
773 0 $t Dissertations Abstracts International $g 85-11B.
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=30996422 $z click for full text (PQDT)