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The Study of the Effect of Microcontrations in the Separation of Blood Cells : = Soft Lithography and Micromilling.

紀錄類型:	書目-語言資料,手稿 : Monograph/item
正題名/作者:	The Study of the Effect of Microcontrations in the Separation of Blood Cells :/
其他題名:	Soft Lithography and Micromilling.
作者:	Lopes, Ana Raquel Jacome.
面頁冊數:	1 online resource (69 pages)
附註:	Source: Masters Abstracts International, Volume: 84-05.
Contained By:	Masters Abstracts International84-05.
標題:	Polymer chemistry. -
電子資源:	click for full text (PQDT)
ISBN:	9798357521828

The Study of the Effect of Microcontrations in the Separation of Blood Cells : = Soft Lithography and Micromilling.
Lopes, Ana Raquel Jacome.

The Study of the Effect of Microcontrations in the Separation of Blood Cells :Soft Lithography and Micromilling. - 1 online resource (69 pages)

Source: Masters Abstracts International, Volume: 84-05.

Thesis (M.Sc.)--Instituto Politecnico de Braganca (Portugal), 2014.

Includes bibliographical references

The detection, separation and manipulation of microparticles and blood cells are important stages for biologic and chemical analysis. A new application in the area of Biomedical Engineering with potential is the development of portables micro devices for diagnostic, those are known as lab-on-chips or blood-on-chips. Their principal function is the separation and identification pathological cells in a blood sample. For example, infected cells with diabetes and malaria, present different deformation indices from healthy ones. This device should be a simple and low cost way to separate pathologic cells from healthy. The goal of this work is to design a geometry containing a microcontraction and test its effect in the cell free layer (CFL) and consequently in the separation of cells from plasma.In a first phase, it is used a soft lithography method for the fabrication of hyperbolic microchannels in PDMS. The hyperbolic microchannels are tested with a hematocrit of 5% of ovine red blood cells. Some results were presented and discussed.A low cost fabrication method was used, consisting in using a micromilling machine to mill in PMMA glass or Plexiglass to fabricate microchannels with simple contractions. The microchannels were tested with 5% of ovine red blood cells. The low cost method was discussed and some results about cell separation were presented.

Electronic reproduction.
Ann Arbor, Mich. :
ProQuest,
2024

Mode of access: World Wide Web

ISBN: 9798357521828Subjects--Topical Terms:

1182163
Polymer chemistry.
Index Terms--Genre/Form:

554714
Electronic books.

The Study of the Effect of Microcontrations in the Separation of Blood Cells : = Soft Lithography and Micromilling.
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504 $a Includes bibliographical references
520 $a The detection, separation and manipulation of microparticles and blood cells are important stages for biologic and chemical analysis. A new application in the area of Biomedical Engineering with potential is the development of portables micro devices for diagnostic, those are known as lab-on-chips or blood-on-chips. Their principal function is the separation and identification pathological cells in a blood sample. For example, infected cells with diabetes and malaria, present different deformation indices from healthy ones. This device should be a simple and low cost way to separate pathologic cells from healthy. The goal of this work is to design a geometry containing a microcontraction and test its effect in the cell free layer (CFL) and consequently in the separation of cells from plasma.In a first phase, it is used a soft lithography method for the fabrication of hyperbolic microchannels in PDMS. The hyperbolic microchannels are tested with a hematocrit of 5% of ovine red blood cells. Some results were presented and discussed.A low cost fabrication method was used, consisting in using a micromilling machine to mill in PMMA glass or Plexiglass to fabricate microchannels with simple contractions. The microchannels were tested with 5% of ovine red blood cells. The low cost method was discussed and some results about cell separation were presented.
520 $a A detecao, separacao e manipulacao de microparticulas e celulas sanguineas sao fases importantes para uma analise quimica e biologica. Uma nova aplicacao na area de Engenharia Biomedica com potencial e o desenvolvimento de microdispositivos portateis para diagnostico, mais conhecidos como "lab-on-chips" ou "blood-on-chips".A principal funcao destes dispositivos e a separacao e identificacao de celulas patologicas numa amostra de sangue. Certas doencas como a diabetes e a malaria alteram os indices de deformacao dos Globulos vermelhos, sendo o objetivo deste dispositivo a separacao das celulas saudaveis das patologicas de uma maneira simples e barata. O objetivo deste trabalho e projetar uma geometria com uma microcontracao e testar o efeito na camada livre de celula e consequentemente a sua eficacia na separacao de celulas do plasma. Numa primeira fase e realizada uma pequena explicacao sobre litografia, assim como os resultados e discussao dos escoamentos com sangue dos canais produzidos em PDMS a partir de um molde em uma wafer feitos por litografia com SU8.Numa segunda fase e explicado brevemente o processo de produzir microcanais, com uma micro-fresadora, em vidro de PMMA. Este processo consistiu em desenhar a geometria, o manuseamento dos softwares e os acabamentos finais nos microcanais fabricados.
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