國立虎尾科技大學 |

Internalization of oligonucleotides into bacterial cells.

紀錄類型:	書目-語言資料,手稿 : Monograph/item
正題名/作者:	Internalization of oligonucleotides into bacterial cells./
作者:	Don, Michelle.
面頁冊數:	1 online resource (66 pages)
附註:	Source: Masters Abstracts International, Volume: 71-10.
Contained By:	Masters Abstracts International71-10.
標題:	Microbiology. -
電子資源:	click for full text (PQDT)
ISBN:	9781109339482

Internalization of oligonucleotides into bacterial cells.
Don, Michelle.

Internalization of oligonucleotides into bacterial cells. - 1 online resource (66 pages)

Source: Masters Abstracts International, Volume: 71-10.

Thesis (M.S.)--California State University, Fullerton, 2009.

Includes bibliographical references

The enzyme aminoglycoside 6'-N-acetyl transferase (AAC(6')-Ib) encoded by the transposon Tn1331 on the pJHCMW1 plasmid, mediates resistance to amikacin and other aminoglycoside antibiotics. Through the use of RNase H mapping and computer prediction software, the secondary structure of aac(6')-Ib mRNA was determined and regions accessible for interaction with antisense oligonucleotides were identified. Oligonucleotides designed to target these regions have been used to inhibit the synthesis of AAC(6')-Ib, decreasing the level of resistance to amikacin. However, a successful and reproducible method of delivery of oligonucleotides to bacterial cells is not yet available. Liposome-mediated delivery of fluorescent labeled phosphorothioate oligonucleotide analogues and plasmid DNA to bacterial cells was attempted in this study. Fluorescent microscopy and antibiotic resistance plates were used as evidence to show internalization of the oligonucleotides or plasmid DNA. A method of liposome encapsulation of DNA was established, however, liposome-mediated delivery of DNA to Escherichia coli and Bacillus subtilis cells was unsuccessful. Delivery of naked phosphorothioate oligonucleotide analogues to Acinetobacter baumanii was successful and internalization was visualized through fluorescence microscopy. This work provides a methodology to test internalization of oligonucleotides into bacterial cells, and contributes to the overall understanding of liposomes as vehicles of DNA delivery and provides the first evidence that Acinetobacter baumanii, as it is the case of A. calcoaceticus and A. baylyi, is naturally competent.

Electronic reproduction.
Ann Arbor, Mich. :
ProQuest,
2024

Mode of access: World Wide Web

ISBN: 9781109339482Subjects--Topical Terms:

591510
Microbiology.
Index Terms--Genre/Form:

554714
Electronic books.

Internalization of oligonucleotides into bacterial cells.
LDR:02869ntm a22003497 4500 001 1148444
005 20240924101513.5
006 m o d
007 cr bn ---uuuuu
008 250605s2009 xx obm 000 0 eng d
020 $a 9781109339482
035 $a (MiAaPQ)AAI1470805
035 $a AAI1470805
040 $a MiAaPQ $b eng $c MiAaPQ $d NTU
100 1 $a Don, Michelle. $3 1474404
245 1 0 $a Internalization of oligonucleotides into bacterial cells.
264 0 $c 2009
300 $a 1 online resource (66 pages)
336 $a text $b txt $2 rdacontent
337 $a computer $b c $2 rdamedia
338 $a online resource $b cr $2 rdacarrier
500 $a Source: Masters Abstracts International, Volume: 71-10.
500 $a Publisher info.: Dissertation/Thesis.
500 $a Advisor: Tolmasky, Marcelo.
502 $a Thesis (M.S.)--California State University, Fullerton, 2009.
504 $a Includes bibliographical references
520 $a The enzyme aminoglycoside 6'-N-acetyl transferase (AAC(6')-Ib) encoded by the transposon Tn1331 on the pJHCMW1 plasmid, mediates resistance to amikacin and other aminoglycoside antibiotics. Through the use of RNase H mapping and computer prediction software, the secondary structure of aac(6')-Ib mRNA was determined and regions accessible for interaction with antisense oligonucleotides were identified. Oligonucleotides designed to target these regions have been used to inhibit the synthesis of AAC(6')-Ib, decreasing the level of resistance to amikacin. However, a successful and reproducible method of delivery of oligonucleotides to bacterial cells is not yet available. Liposome-mediated delivery of fluorescent labeled phosphorothioate oligonucleotide analogues and plasmid DNA to bacterial cells was attempted in this study. Fluorescent microscopy and antibiotic resistance plates were used as evidence to show internalization of the oligonucleotides or plasmid DNA. A method of liposome encapsulation of DNA was established, however, liposome-mediated delivery of DNA to Escherichia coli and Bacillus subtilis cells was unsuccessful. Delivery of naked phosphorothioate oligonucleotide analogues to Acinetobacter baumanii was successful and internalization was visualized through fluorescence microscopy. This work provides a methodology to test internalization of oligonucleotides into bacterial cells, and contributes to the overall understanding of liposomes as vehicles of DNA delivery and provides the first evidence that Acinetobacter baumanii, as it is the case of A. calcoaceticus and A. baylyi, is naturally competent.
533 $a Electronic reproduction. $b Ann Arbor, Mich. : $c ProQuest, $d 2024
538 $a Mode of access: World Wide Web
650 4 $a Microbiology. $3 591510
650 4 $a Cellular biology. $3 1148666
650 4 $a Molecular biology. $3 583443
655 7 $a Electronic books. $2 local $3 554714
690 $a 0307
690 $a 0379
690 $a 0410
710 2 $a California State University, Fullerton. $3 1189459
710 2 $a ProQuest Information and Learning Co. $3 1178819
773 0 $t Masters Abstracts International $g 71-10.
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=1470805 $z click for full text (PQDT)