國立虎尾科技大學 |

Vascular Cell and Membrane Permeability : = Biophysical Mechanisms and Experimental Techniques.

紀錄類型:	書目-語言資料,手稿 : Monograph/item
正題名/作者:	Vascular Cell and Membrane Permeability :/
其他題名:	Biophysical Mechanisms and Experimental Techniques.
作者:	Younger, Scott.
面頁冊數:	1 online resource (139 pages)
附註:	Source: Dissertations Abstracts International, Volume: 85-05, Section: B.
Contained By:	Dissertations Abstracts International85-05B.
標題:	Biophysics. -
電子資源:	click for full text (PQDT)
ISBN:	9798380623964

Vascular Cell and Membrane Permeability : = Biophysical Mechanisms and Experimental Techniques.
Younger, Scott.

Vascular Cell and Membrane Permeability :Biophysical Mechanisms and Experimental Techniques. - 1 online resource (139 pages)

Source: Dissertations Abstracts International, Volume: 85-05, Section: B.

Thesis (Ph.D.)--The University of Arizona, 2023.

Includes bibliographical references

Semipermeable cellular barriers play fundamental roles in human physiology and pathobiology. These include the plasma membrane which separates the cellular cytoplasm from the extracellular space and the endothelial cell barrier which separates the internal components of tissue from the circulating blood. The loss of plasma membranes barrier integrity leads to loss of ionic homeostasis, increased permeability through paracellular gaps and to potentially irreversible organ dysfunction. This dissertation describes the biophysical mechanisms and techniques employed toinvestigate mechanisms of permeability regulation involving: A) the amyloid protein, Medin, in pore formation in lipid bilayers which leads to increased ionic permeability, and; B) key cytoskeletal regulator proteins in paracellular gap formation/restoration and modulation of vascular endothelial cell (EC) stiffness including the key cytoskeletal regulator protein, EVL, a member of the ENA-VASP family of proteins. These were tested using electrophysiology, optical microscopy, atomic force microscopy (AFM), and other biophysical techniques. The combination of optical, electrical, and force measurements provided unique insights into the mechanisms underpinning barrier dysfunction and recovery. The tools and techniques created during this project, including polymer-based constructs for the formation of paracellular gaps to mimic tissue injury, a force measurement device to determine lamellipodial protrusion forces based on combining AFM and optical microscopy components together, were designed to be adaptable to study similar barrier-effectors. Thus, the findings described here, as well as the experimental and analysis techniques developed during this work, have the potential to substantially contribute to the understanding of vascular and plasma membrane barrier regulation that influence the severity of multiple inflammatory disorders.

Electronic reproduction.
Ann Arbor, Mich. :
ProQuest,
2024

Mode of access: World Wide Web

ISBN: 9798380623964Subjects--Topical Terms:

581576
Biophysics.
Subjects--Index Terms:

Actin effectorIndex Terms--Genre/Form:

554714
Electronic books.

Vascular Cell and Membrane Permeability : = Biophysical Mechanisms and Experimental Techniques.
LDR:03353ntm a22004217 4500 001 1149369
005 20241015112531.5
006 m o d
007 cr bn ---uuuuu
008 250605s2023 xx obm 000 0 eng d
020 $a 9798380623964
035 $a (MiAaPQ)AAI30636159
035 $a AAI30636159
040 $a MiAaPQ $b eng $c MiAaPQ $d NTU
100 1 $a Younger, Scott. $3 1475572
245 1 0 $a Vascular Cell and Membrane Permeability : $b Biophysical Mechanisms and Experimental Techniques.
264 0 $c 2023
300 $a 1 online resource (139 pages)
336 $a text $b txt $2 rdacontent
337 $a computer $b c $2 rdamedia
338 $a online resource $b cr $2 rdacarrier
500 $a Source: Dissertations Abstracts International, Volume: 85-05, Section: B.
500 $a Advisor: Garcia, Joe G. N.
502 $a Thesis (Ph.D.)--The University of Arizona, 2023.
504 $a Includes bibliographical references
520 $a Semipermeable cellular barriers play fundamental roles in human physiology and pathobiology. These include the plasma membrane which separates the cellular cytoplasm from the extracellular space and the endothelial cell barrier which separates the internal components of tissue from the circulating blood. The loss of plasma membranes barrier integrity leads to loss of ionic homeostasis, increased permeability through paracellular gaps and to potentially irreversible organ dysfunction. This dissertation describes the biophysical mechanisms and techniques employed toinvestigate mechanisms of permeability regulation involving: A) the amyloid protein, Medin, in pore formation in lipid bilayers which leads to increased ionic permeability, and; B) key cytoskeletal regulator proteins in paracellular gap formation/restoration and modulation of vascular endothelial cell (EC) stiffness including the key cytoskeletal regulator protein, EVL, a member of the ENA-VASP family of proteins. These were tested using electrophysiology, optical microscopy, atomic force microscopy (AFM), and other biophysical techniques. The combination of optical, electrical, and force measurements provided unique insights into the mechanisms underpinning barrier dysfunction and recovery. The tools and techniques created during this project, including polymer-based constructs for the formation of paracellular gaps to mimic tissue injury, a force measurement device to determine lamellipodial protrusion forces based on combining AFM and optical microscopy components together, were designed to be adaptable to study similar barrier-effectors. Thus, the findings described here, as well as the experimental and analysis techniques developed during this work, have the potential to substantially contribute to the understanding of vascular and plasma membrane barrier regulation that influence the severity of multiple inflammatory disorders.
533 $a Electronic reproduction. $b Ann Arbor, Mich. : $c ProQuest, $d 2024
538 $a Mode of access: World Wide Web
650 4 $a Biophysics. $3 581576
650 4 $a Molecular biology. $3 583443
650 4 $a Pathology. $3 668553
650 4 $a Physiology. $3 673386
650 4 $a Biomedical engineering. $3 588770
653 $a Actin effector
653 $a Amyloid
653 $a Barrier restoration
653 $a Cytoskeleton
653 $a Electrophysiology
655 7 $a Electronic books. $2 local $3 554714
690 $a 0541
690 $a 0786
690 $a 0307
690 $a 0571
690 $a 0719
710 2 $a The University of Arizona. $b Biomedical Engineering. $3 1475573
710 2 $a ProQuest Information and Learning Co. $3 1178819
773 0 $t Dissertations Abstracts International $g 85-05B.
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=30636159 $z click for full text (PQDT)