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Effects of Biohydrogenation Intermediates on Adipogenesis and Lipogenesis of Primary Bovine Preadipocytes and Dedifferentiated Mature Adipocytes.

紀錄類型:	書目-語言資料,手稿 : Monograph/item
正題名/作者:	Effects of Biohydrogenation Intermediates on Adipogenesis and Lipogenesis of Primary Bovine Preadipocytes and Dedifferentiated Mature Adipocytes./
作者:	Park, Kihoon.
面頁冊數:	1 online resource (89 pages)
附註:	Source: Masters Abstracts International, Volume: 85-03.
Contained By:	Masters Abstracts International85-03.
標題:	Cellular biology. -
電子資源:	click for full text (PQDT)
ISBN:	9798380364324

Effects of Biohydrogenation Intermediates on Adipogenesis and Lipogenesis of Primary Bovine Preadipocytes and Dedifferentiated Mature Adipocytes.
Park, Kihoon.

Effects of Biohydrogenation Intermediates on Adipogenesis and Lipogenesis of Primary Bovine Preadipocytes and Dedifferentiated Mature Adipocytes. - 1 online resource (89 pages)

Source: Masters Abstracts International, Volume: 85-03.

Thesis (M.S.)--University of California, Davis, 2023.

Includes bibliographical references

High-grain diets are commonly fed to increase the deposition of marbling fat in finishing beef cattle. While feeding grain-based diets is essential for producing high-quality beef, it can shift ruminal biohydrogenation pathways towards t10-18:1 and t10, c12-CLA as intermediates instead of t11-18:1 and c9, t11-CLA. The t10, c12-CLA has been consistently shown to have anti-lipogenic properties in bovine adipocyte cultures. However, bioactivities of other biohydrogenation intermediates (BHI) including t10-18:1, t11-18:1, and c9, t11-CLA are still unclear. Therefore, we sought to provide a clearer view of adipogenic and lipogenic effects of key BHI (t10, c12-CLA, c9, t11-CLA, t10-18:1, and t11-18:1) on stromovascular fraction (SVF) and dedifferentiated fat (DFAT) cells obtained from beef intramuscular adipose tissue. Confluent SVF and DFAT cells were exposed to media containing adipogenic inducers (dexamethasone, 3-isobutyl-1-methylxanthine) and 0, 5, 10, or 20 µM of BHI treatments for 6 days followed by Oil Red O staining, fatty acid, qPCR, and GPDH activity assays. DFAT-derived mature adipocytes, regardless of the BHI treatment, showed a higher supplemented fatty acid incorporation and CCAAT/Enhancer-binding protein α (C/EBP α) expression compared to SVF adipocytes. Oil Red O staining showed that t10-18:1 treatment reduced lipid accumulation compared to other BHI treatments in both SVF and DFAT adipocytes, without impacting lipogenic genes. Conversely, t10, c12-CLA significantly reduced SCD1 expression compared to other BHI treatments in both cell models, which was accompanied by reduced 18:1/18:0 and 16:1/16:0 ratios. In conclusion, our findings suggest that both t10-18:1 and t10, c12 CLA may negatively impact marbling development in beef cattle, while t11-18:1 and c9, t11-CLA are neutral in this regard. More in vitro and in vivo studies are needed to elucidate the mechanisms by which t10-18:1 and t10, c12-18:2 may affect marbling development in beef cattle, including their potential synergistic effects on adipogenesis and lipogenesis in bovine adipocytes.

Electronic reproduction.
Ann Arbor, Mich. :
ProQuest,
2024

Mode of access: World Wide Web

ISBN: 9798380364324Subjects--Topical Terms:

1148666
Cellular biology.
Subjects--Index Terms:

Bovine adipocytesIndex Terms--Genre/Form:

554714
Electronic books.

Effects of Biohydrogenation Intermediates on Adipogenesis and Lipogenesis of Primary Bovine Preadipocytes and Dedifferentiated Mature Adipocytes.
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