國立虎尾科技大學 |

Determining the Relationship Between Timing and Presence of Algae Cells, Protein Metabolism, and the Induction of Physiological Plasticity in the Pacific Sand Dollar, Dendraster excentricus.

紀錄類型:	書目-語言資料,手稿 : Monograph/item
正題名/作者:	Determining the Relationship Between Timing and Presence of Algae Cells, Protein Metabolism, and the Induction of Physiological Plasticity in the Pacific Sand Dollar, Dendraster excentricus./
作者:	Vitagliano, Juliana.
面頁冊數:	1 online resource (52 pages)
附註:	Source: Masters Abstracts International, Volume: 85-06.
Contained By:	Masters Abstracts International85-06.
標題:	Biology. -
電子資源:	click for full text (PQDT)
ISBN:	9798381169065

Determining the Relationship Between Timing and Presence of Algae Cells, Protein Metabolism, and the Induction of Physiological Plasticity in the Pacific Sand Dollar, Dendraster excentricus.
Vitagliano, Juliana.

Determining the Relationship Between Timing and Presence of Algae Cells, Protein Metabolism, and the Induction of Physiological Plasticity in the Pacific Sand Dollar, Dendraster excentricus. - 1 online resource (52 pages)

Source: Masters Abstracts International, Volume: 85-06.

Thesis (M.S.)--California State University, Long Beach, 2023.

Includes bibliographical references

Planktotrophic larvae, like that of Dendraster excentricus, are reliant on the heterogenous marine environment to provide food (algae cells) to fuel them throughout their planktonic developmental life stages. As the ability to acquire sufficient amounts of exogenous food impacts the rate of planktonic development by inducing various plasticity responses (morphological, physiological and biochemical), it is important to understand exactly how and when these plasticity responses are initiated. While plasticity responses are thought to be adaptive in nature, they can only be initiated if the organism possesses a reliable sensory mechanism by which to evaluate prevailing environmental conditions. Previous research in echinoid larvae has determined that environmental algae concentration is evaluated during pre-feeding embryonic stages and is linked to chemical secretions from the algal cells themselves. This pre-feeding exposure to future feeding conditions allows larvae to initiate arm length investment at an environmentally appropriate level for efficient feeding. This morphological plasticity response of growing longer larval arms in food-limited environments is supported by additional changes in physiological pathways relating to growth efficiency, including protein metabolism. The purpose of the current research was to determine how early in development the presence of algae cells are capable of inducing physiological plasticity in protein metabolism. Rates of total alanine transport, rates of protein synthesis, and measurements of protein biomass were determined in embryos (blastula [12 hours post-fertilization], and gastrula [24 hours post-fertilization]), and resulting early-stage larvae (3 days post-fertilization) in response to (1) embryonic algae exposure, (2) larval feeding treatment, and (3) the interaction between embryonic and larval algae treatments to understand how and when algae presence is capable of inducing physiological plasticity responses. Algae exposure during feeding (larval) development demonstrated that fed larvae increase both rates of protein synthesis (F = 41.332, df = 1, p < 0.001) and the amount of protein deposited as biomass (F = 33.116, df = 1, p = 0.001), demonstrating strong plasticity response to feeding environment during feeding development. Surprisingly, I did not find a significant effect of larval feeding treatment and rates of total alanine transport (F = 0.37, df = 1, p > 0.05) that has been identified to occur in previous studies. Embryos were unable to respond to the presence of algae cells in a manner that affected their embryonic protein metabolism. Though embryonic exposure was unable to affect embryonic protein metabolism, it is possible that embryos are still sensitive to their future feeding environment, as we observed that larvae resulting from embryos exposed to algae had higher amounts of protein biomass than larvae resulting from embryos reared in the absence of algae (F = 14.106, df = 1, p < 0.01); however, I did not observe any effects of embryonic algae exposure on larval rates of protein synthesis, or rates of total alanine transport. These results suggest that while embryos may be able to detect their future feeding conditions, they are unable to initiate physiological plasticity responses until they have achieved a feeding larval stage. While it is still unknown exactly how embryos are capable of sensing their future feeding environment, this research has defined when these plastic responses are being initiated.

Electronic reproduction.
Ann Arbor, Mich. :
ProQuest,
2024

Mode of access: World Wide Web

ISBN: 9798381169065Subjects--Topical Terms:

599573
Biology.
Subjects--Index Terms:

Dendraster excentricusIndex Terms--Genre/Form:

554714
Electronic books.

Determining the Relationship Between Timing and Presence of Algae Cells, Protein Metabolism, and the Induction of Physiological Plasticity in the Pacific Sand Dollar, Dendraster excentricus.
LDR:05099ntm a22004337 4500 001 1150244
005 20241028114726.5
006 m o d
007 cr bn ---uuuuu
008 250605s2023 xx obm 000 0 eng d
020 $a 9798381169065
035 $a (MiAaPQ)AAI30418290
035 $a AAI30418290
040 $a MiAaPQ $b eng $c MiAaPQ $d NTU
100 1 $a Vitagliano, Juliana. $3 1476690
245 1 0 $a Determining the Relationship Between Timing and Presence of Algae Cells, Protein Metabolism, and the Induction of Physiological Plasticity in the Pacific Sand Dollar, Dendraster excentricus.
264 0 $c 2023
300 $a 1 online resource (52 pages)
336 $a text $b txt $2 rdacontent
337 $a computer $b c $2 rdamedia
338 $a online resource $b cr $2 rdacarrier
500 $a Source: Masters Abstracts International, Volume: 85-06.
500 $a Advisor: Pace, Douglas.
502 $a Thesis (M.S.)--California State University, Long Beach, 2023.
504 $a Includes bibliographical references
520 $a Planktotrophic larvae, like that of Dendraster excentricus, are reliant on the heterogenous marine environment to provide food (algae cells) to fuel them throughout their planktonic developmental life stages. As the ability to acquire sufficient amounts of exogenous food impacts the rate of planktonic development by inducing various plasticity responses (morphological, physiological and biochemical), it is important to understand exactly how and when these plasticity responses are initiated. While plasticity responses are thought to be adaptive in nature, they can only be initiated if the organism possesses a reliable sensory mechanism by which to evaluate prevailing environmental conditions. Previous research in echinoid larvae has determined that environmental algae concentration is evaluated during pre-feeding embryonic stages and is linked to chemical secretions from the algal cells themselves. This pre-feeding exposure to future feeding conditions allows larvae to initiate arm length investment at an environmentally appropriate level for efficient feeding. This morphological plasticity response of growing longer larval arms in food-limited environments is supported by additional changes in physiological pathways relating to growth efficiency, including protein metabolism. The purpose of the current research was to determine how early in development the presence of algae cells are capable of inducing physiological plasticity in protein metabolism. Rates of total alanine transport, rates of protein synthesis, and measurements of protein biomass were determined in embryos (blastula [12 hours post-fertilization], and gastrula [24 hours post-fertilization]), and resulting early-stage larvae (3 days post-fertilization) in response to (1) embryonic algae exposure, (2) larval feeding treatment, and (3) the interaction between embryonic and larval algae treatments to understand how and when algae presence is capable of inducing physiological plasticity responses. Algae exposure during feeding (larval) development demonstrated that fed larvae increase both rates of protein synthesis (F = 41.332, df = 1, p < 0.001) and the amount of protein deposited as biomass (F = 33.116, df = 1, p = 0.001), demonstrating strong plasticity response to feeding environment during feeding development. Surprisingly, I did not find a significant effect of larval feeding treatment and rates of total alanine transport (F = 0.37, df = 1, p > 0.05) that has been identified to occur in previous studies. Embryos were unable to respond to the presence of algae cells in a manner that affected their embryonic protein metabolism. Though embryonic exposure was unable to affect embryonic protein metabolism, it is possible that embryos are still sensitive to their future feeding environment, as we observed that larvae resulting from embryos exposed to algae had higher amounts of protein biomass than larvae resulting from embryos reared in the absence of algae (F = 14.106, df = 1, p < 0.01); however, I did not observe any effects of embryonic algae exposure on larval rates of protein synthesis, or rates of total alanine transport. These results suggest that while embryos may be able to detect their future feeding conditions, they are unable to initiate physiological plasticity responses until they have achieved a feeding larval stage. While it is still unknown exactly how embryos are capable of sensing their future feeding environment, this research has defined when these plastic responses are being initiated.
533 $a Electronic reproduction. $b Ann Arbor, Mich. : $c ProQuest, $d 2024
538 $a Mode of access: World Wide Web
650 4 $a Biology. $3 599573
650 4 $a Physiology. $3 673386
650 4 $a Ecology. $3 575279
650 4 $a Biochemistry. $3 582831
650 4 $a Biological oceanography. $3 1178855
653 $a Dendraster excentricus
653 $a Embryo
653 $a Planktotrophic larvae
653 $a Physiological plasticity
653 $a Protein metabolism
653 $a Protein synthesis
655 7 $a Electronic books. $2 local $3 554714
690 $a 0306
690 $a 0487
690 $a 0416
690 $a 0329
690 $a 0719
710 2 $a ProQuest Information and Learning Co. $3 1178819
710 2 $a California State University, Long Beach. $b Biological Sciences. $3 1182737
773 0 $t Masters Abstracts International $g 85-06.
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=30418290 $z click for full text (PQDT)