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The experimental evolution of host adaptation of the emerging pathogen Burkholderia cenocepacia.

Record Type:	Language materials, manuscript : Monograph/item
Title/Author:	The experimental evolution of host adaptation of the emerging pathogen Burkholderia cenocepacia./
Author:	Ellis, Crystal Nicole.
Description:	1 online resource (94 pages)
Notes:	Source: Masters Abstracts International, Volume: 70-03.
Contained By:	Masters Abstracts International70-03.
Subject:	Genetics. -
Online resource:	click for full text (PQDT)
ISBN:	9780549552086

The experimental evolution of host adaptation of the emerging pathogen Burkholderia cenocepacia.
Ellis, Crystal Nicole.

The experimental evolution of host adaptation of the emerging pathogen Burkholderia cenocepacia. - 1 online resource (94 pages)

Source: Masters Abstracts International, Volume: 70-03.

Thesis (M.S.)--University of New Hampshire, 2008.

Includes bibliographical references

I investigated the ability of Burkholderia cenocepacia, an opportunistic bacterial pathogen, to adapt to a host. Studies have identified trade-offs associated with environmental adaptation, but few have investigated host adaptation. Consequently, I studied effects of adaptation by B. cenocepacia to onions (Allium cepa) on the ability to kill Caenorhabditis elegans. I hypothesized that adaptation to onions would reduce virulence in C. elegans. I evolved twelve populations of bacteria in onion tissue medium for 500 generations. Then, I quantified fitness differences between evolved and ancestral populations by direct competition, having developed molecular marking techniques to discriminate among competitors. Competitions revealed fitness increases in nine populations. Next, I measured virulence against C. elegans of each population and observed a reduced worm killing ability. I also quantified pleiotropic effects of adaptation related to virulence. In conclusion, I supported that adaptation of B. cenocepacia to one host resulted in decreased virulence in another host.

Electronic reproduction.
Ann Arbor, Mich. :
ProQuest,
2024

Mode of access: World Wide Web

ISBN: 9780549552086Subjects--Topical Terms:

578972
Genetics.
Index Terms--Genre/Form:

554714
Electronic books.

The experimental evolution of host adaptation of the emerging pathogen Burkholderia cenocepacia.
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100 1 $a Ellis, Crystal Nicole. $3 1477620
245 1 4 $a The experimental evolution of host adaptation of the emerging pathogen Burkholderia cenocepacia.
264 0 $c 2008
300 $a 1 online resource (94 pages)
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500 $a Source: Masters Abstracts International, Volume: 70-03.
500 $a Publisher info.: Dissertation/Thesis.
500 $a Advisor: Cooper, Vaughn S.
502 $a Thesis (M.S.)--University of New Hampshire, 2008.
504 $a Includes bibliographical references
520 $a I investigated the ability of Burkholderia cenocepacia, an opportunistic bacterial pathogen, to adapt to a host. Studies have identified trade-offs associated with environmental adaptation, but few have investigated host adaptation. Consequently, I studied effects of adaptation by B. cenocepacia to onions (Allium cepa) on the ability to kill Caenorhabditis elegans. I hypothesized that adaptation to onions would reduce virulence in C. elegans. I evolved twelve populations of bacteria in onion tissue medium for 500 generations. Then, I quantified fitness differences between evolved and ancestral populations by direct competition, having developed molecular marking techniques to discriminate among competitors. Competitions revealed fitness increases in nine populations. Next, I measured virulence against C. elegans of each population and observed a reduced worm killing ability. I also quantified pleiotropic effects of adaptation related to virulence. In conclusion, I supported that adaptation of B. cenocepacia to one host resulted in decreased virulence in another host.
533 $a Electronic reproduction. $b Ann Arbor, Mich. : $c ProQuest, $d 2024
538 $a Mode of access: World Wide Web
650 4 $a Genetics. $3 578972
650 4 $a Microbiology. $3 591510
655 7 $a Electronic books. $2 local $3 554714
690 $a 0369
690 $a 0410
710 2 $a ProQuest Information and Learning Co. $3 1178819
710 2 $a University of New Hampshire. $3 1437613
773 0 $t Masters Abstracts International $g 70-03.
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=1454999 $z click for full text (PQDT)