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Micro- and Nanofluidic Devices for the Sizing and Assembly of Nanoscale Particles.

Record Type:	Language materials, manuscript : Monograph/item
Title/Author:	Micro- and Nanofluidic Devices for the Sizing and Assembly of Nanoscale Particles./
Author:	Young, Tanner Wayne.
Description:	1 online resource (203 pages)
Notes:	Source: Dissertations Abstracts International, Volume: 86-02, Section: B.
Contained By:	Dissertations Abstracts International86-02B.
Subject:	Analytical chemistry. -
Online resource:	click for full text (PQDT)
ISBN:	9798383628409

Micro- and Nanofluidic Devices for the Sizing and Assembly of Nanoscale Particles.
Young, Tanner Wayne.

Micro- and Nanofluidic Devices for the Sizing and Assembly of Nanoscale Particles. - 1 online resource (203 pages)

Source: Dissertations Abstracts International, Volume: 86-02, Section: B.

Thesis (Ph.D.)--Indiana University, 2024.

Includes bibliographical references

Micro- and nanofluidic devices provide a platform for a multitude of applications that offer improved limits of detection, increased measurement precision, and higher throughput. Currently, many nanoscale particles are often characterized by ensemble methods, such as dynamic light scattering and multi-angle laser light scattering. Ensemble measurements often obscure the intrinsic heterogeneity of samples, and species present in low abundance are often overlooked. For these reasons, characterization and assembly of nanoparticles at the single particle level is desirable. Here, two techniques are discussed: resistive-pulse sensing on in-plane nanofluidic devices to detect and characterize single particles and two-phase flow on microfluidic devices to generate superparticle assemblies with decreased heterogeneity and higher throughput.Resistive-pulse sensing is a label-free, single-particle detection technique for determining the size and surface charge of biotic particles (e.g., extracellular vesicles and liposomes) and abiotic particles (e.g., silica and polystyrene). In-plane resistive-pulse devices can contain multiple functional elements (e.g., nanofilters, nanochannels, and nanopores) integrated together to improve measurement precision and enhance device longevity. Size measurements of extracellular vesicles derived from bovine milk and a human cancer cell line with diameters ranging from 50-160 nm indicated minimal difference between samples; however, their zeta potentials (ζ) differed by 2-fold, -8 ± 1 and -4 ± 1 mV, respectively. In addition, liposomes extruded through pore diameters from 50 to 100 nm were analyzed individually and compared to ensemble measurements.Two-phase flow generated on microfluidic devices formed monodisperse microdroplets with a relative standard deviation (RSD) ≤ 1% These devices were used to assemble superparticles with diameters from submicrometers to micrometers with RSDs ≤ 30% and with icosahedral, rhombic, and face-centered cubic geometries. Additionally, symmetric and asymmetric droplet splitting provided increased throughput and multiple monodisperse droplet populations. The increase in droplet throughput did not impact either the droplet size distributions or resulting superparticle distributions.

Electronic reproduction.
Ann Arbor, Mich. :
ProQuest,
2024

Mode of access: World Wide Web

ISBN: 9798383628409Subjects--Topical Terms:

1182118
Analytical chemistry.
Subjects--Index Terms:

MicrodropletsIndex Terms--Genre/Form:

554714
Electronic books.

Micro- and Nanofluidic Devices for the Sizing and Assembly of Nanoscale Particles.
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520 $a Micro- and nanofluidic devices provide a platform for a multitude of applications that offer improved limits of detection, increased measurement precision, and higher throughput. Currently, many nanoscale particles are often characterized by ensemble methods, such as dynamic light scattering and multi-angle laser light scattering. Ensemble measurements often obscure the intrinsic heterogeneity of samples, and species present in low abundance are often overlooked. For these reasons, characterization and assembly of nanoparticles at the single particle level is desirable. Here, two techniques are discussed: resistive-pulse sensing on in-plane nanofluidic devices to detect and characterize single particles and two-phase flow on microfluidic devices to generate superparticle assemblies with decreased heterogeneity and higher throughput.Resistive-pulse sensing is a label-free, single-particle detection technique for determining the size and surface charge of biotic particles (e.g., extracellular vesicles and liposomes) and abiotic particles (e.g., silica and polystyrene). In-plane resistive-pulse devices can contain multiple functional elements (e.g., nanofilters, nanochannels, and nanopores) integrated together to improve measurement precision and enhance device longevity. Size measurements of extracellular vesicles derived from bovine milk and a human cancer cell line with diameters ranging from 50-160 nm indicated minimal difference between samples; however, their zeta potentials (ζ) differed by 2-fold, -8 ± 1 and -4 ± 1 mV, respectively. In addition, liposomes extruded through pore diameters from 50 to 100 nm were analyzed individually and compared to ensemble measurements.Two-phase flow generated on microfluidic devices formed monodisperse microdroplets with a relative standard deviation (RSD) ≤ 1% These devices were used to assemble superparticles with diameters from submicrometers to micrometers with RSDs ≤ 30% and with icosahedral, rhombic, and face-centered cubic geometries. Additionally, symmetric and asymmetric droplet splitting provided increased throughput and multiple monodisperse droplet populations. The increase in droplet throughput did not impact either the droplet size distributions or resulting superparticle distributions.
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