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Characterizing Memory Trace Reactivation and Biosynthetic Changes During Post-learning Sleep in the Hippocampus.

Record Type:	Language materials, manuscript : Monograph/item
Title/Author:	Characterizing Memory Trace Reactivation and Biosynthetic Changes During Post-learning Sleep in the Hippocampus./
Author:	Wang, Lijing.
Description:	1 online resource (122 pages)
Notes:	Source: Dissertations Abstracts International, Volume: 85-03, Section: B.
Contained By:	Dissertations Abstracts International85-03B.
Subject:	Neurosciences. -
Online resource:	click for full text (PQDT)
ISBN:	9798380374408

Characterizing Memory Trace Reactivation and Biosynthetic Changes During Post-learning Sleep in the Hippocampus.
Wang, Lijing.

Characterizing Memory Trace Reactivation and Biosynthetic Changes During Post-learning Sleep in the Hippocampus. - 1 online resource (122 pages)

Source: Dissertations Abstracts International, Volume: 85-03, Section: B.

Thesis (Ph.D.)--University of Michigan, 2023.

Includes bibliographical references

Over a century of research in both animal models and human subjects shows that sleep may improve, while sleep loss may disrupt, memory task performance. One prevailing hypothesis, active systems consolidation (ASC), posits that during sleep, neurons encoding newly acquired memories are reactivated, and the connections within the network get strengthened, resulting in the formation of a more persistent long-term memory. My thesis tests this hypothesis by quantifying the reactivation of memory traces in the mouse hippocampus during post-learning sleep. I also characterize learning- and sleep-induced biosynthetic changes in different subregions of the hippocampus to clarify the molecular mechanisms associated with this reactivation, in the context of sleep-dependent contextual fear memory consolidation.Using the targeted recombination in active populations (TRAP) mouse line, I quantified reactivation of hippocampal context-activated engram neurons following contextual fear conditioning (CFC) and subsequent sleep or sleep deprivation (SD). I find that compared with SD, post-CFC sleep promoted reactivation of more engram cells in the hippocampal dentate gyrus (DG). Interestingly, this reactivation had a subregion-specific pattern, with significantly higher proportion of sleep-dependent reactivation in the inferior blade of DG compared with the superior blade.To characterize learning- and sleep-induced biosynthetic changes in the hippocampus, I used spatial transcriptomics and protein profiling to measure expression changes in the hippocampal DG superior blade, inferior blade, hilus, CA1, and CA3 areas. I found that SD differentially altered the expression of genes in each hippocampal subregion, and hence, differentially impacted various biological pathways in these subregions. I also found that several hours after learning, CFC-induced transcriptomic changes are restricted to the hippocampal DG granule cell layers, and are particularly affecting the synaptic components of neurons. Together, the results identify spatially divergent effects of learning and sleep in hippocampal subregions, which clarify the cellular- and network-level processes altered by sleep disruption.

Electronic reproduction.
Ann Arbor, Mich. :
ProQuest,
2024

Mode of access: World Wide Web

ISBN: 9798380374408Subjects--Topical Terms:

593561
Neurosciences.
Subjects--Index Terms:

SleepIndex Terms--Genre/Form:

554714
Electronic books.

Characterizing Memory Trace Reactivation and Biosynthetic Changes During Post-learning Sleep in the Hippocampus.
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