國立虎尾科技大學 |

Directed evolution

紀錄類型:	書目-語言資料,印刷品 : Monograph/item
正題名/作者:	Directed evolution / Aaron M. Leconte and Clair M. Colee, authors.
作者:	Leconte, Aaron M.,
其他作者:	Colee, Clair M.,
面頁冊數:	1 online resource :illustrations (some color). :
標題:	Nucleic acids - Synthesis. -
電子資源:	https://dx.doi.org/10.1021/acsinfocus.7e8004
ISBN:	9780841296374

Directed evolution
Leconte, Aaron M.,

Directed evolution [electronic resource] /Aaron M. Leconte and Clair M. Colee, authors. - 1 online resource :illustrations (some color). - ACS in focus,2691-8307. - ACS in focus,.

Includes bibliographical references and index.

Overview --

"While the field of directed evolution is vast and varied, it comes from a simple principle: to apply the principles of biological evolution in the laboratory to generate useful molecules. This widely used technique has yielded blockbuster drugs, essential biological tools, and many more useful chemicals. Considering its impact, it's unsurprising that the 2018 Chemistry Nobel Prize was awarded for the use of directed evolution of proteins. Directed Evolution is an excellent starting point for any scientist interested in directed evolution. This primer aims to provide readers with the foundational knowledge and vocabulary that enables them to tackle the literature and begin designing their projects. Directed evolution, as a field, can be challenging to start in; one of the biggest challenges to starting work in this field is that it requires a different style of thinking than many areas of chemistry. CHAPTER 1 explains this different mentality and then gives an overview of directed evolution: what it is, why it is useful, how evolution is applied in the lab, and how evolution can be applied for human benefit. Given the diversity and quantity of methods and approaches in directed evolution, the remaining chapters focus on helping the readers find the right approach to their specific problem by outlining the basic thought process and classical methods in directed evolution. Specifically, CHAPTER 2 discusses applying directed evolution to nucleic acids. Nucleic acid evolution has several unique features that make it easier to understand conceptually, making it a good starting point for understanding how to think about and leverage evolution. CHAPTERS 3 through 6 cover approaches to evolving proteins. CHAPTER 3 discusses the strengths and weaknesses of different approaches to the diversification of proteins, giving the reader a framework for designing libraries. CHAPTERS 4, 5, and 6 introduce different approaches to sorting by applying selection pressures to find the best variant from a large complex library. Upon completing this primer, the reader will have a theoretical basis to design and execute a directed evolution campaign. They will have the core knowledge to dive into the larger world of directed evolution and begin designing projects that leverage this powerful technique."--

ISBN: 9780841296374

Standard No.: 10.1021/acsinfocus.7e8004doiSubjects--Topical Terms:

1483794
Nucleic acids
--Synthesis.Index Terms--Genre/Form:

554714
Electronic books.

LC Class. No.: TP248.2 / .L433 2024eb

Dewey Class. No.: 660.6

Directed evolution
LDR:03523nam a2200301 i 4500 001 1155763
003 DACS
005 20240524120307.0
008 250627s2024 dcua ob 101 0 eng d
020 $a 9780841296374 $q electronic
024 7 # $a 10.1021/acsinfocus.7e8004 $2 doi
035 $a (CaBNVSL)slc00005965
035 $a 9780841296374
040 $a NjRocCCS $b eng $e rda $c NjRocCCS $d CaBNVSL
050 # 4 $a TP248.2 $b .L433 2024eb
072 7 $a SCI010000 $2 bisacsh
082 0 0 $a 660.6 $2 23
100 1 $a Leconte, Aaron M., $e author. $u Keck Science Department of Claremont McKenna, Pitzer, and Scripps Colleges. $3 1483792
245 1 0 $a Directed evolution $c Aaron M. Leconte and Clair M. Colee, authors. $h [electronic resource] /
264 1 $a Washington, DC, USA : $b American Chemical Society, $c 2024.
300 $a 1 online resource : $b illustrations (some color).
336 $a text $2 rdacontent
337 $a computer $2 rdamedia
338 $a online resource $2 rdacarrier
490 1 $a ACS in focus, $x 2691-8307
504 $a Includes bibliographical references and index.
505 0 0 $t Overview -- $t Nucleic Acids -- $t Diversification -- $t Screens -- $t In Vitro Selections -- $t In Vivo Selections.
520 3 $a "While the field of directed evolution is vast and varied, it comes from a simple principle: to apply the principles of biological evolution in the laboratory to generate useful molecules. This widely used technique has yielded blockbuster drugs, essential biological tools, and many more useful chemicals. Considering its impact, it's unsurprising that the 2018 Chemistry Nobel Prize was awarded for the use of directed evolution of proteins. Directed Evolution is an excellent starting point for any scientist interested in directed evolution. This primer aims to provide readers with the foundational knowledge and vocabulary that enables them to tackle the literature and begin designing their projects. Directed evolution, as a field, can be challenging to start in; one of the biggest challenges to starting work in this field is that it requires a different style of thinking than many areas of chemistry. CHAPTER 1 explains this different mentality and then gives an overview of directed evolution: what it is, why it is useful, how evolution is applied in the lab, and how evolution can be applied for human benefit. Given the diversity and quantity of methods and approaches in directed evolution, the remaining chapters focus on helping the readers find the right approach to their specific problem by outlining the basic thought process and classical methods in directed evolution. Specifically, CHAPTER 2 discusses applying directed evolution to nucleic acids. Nucleic acid evolution has several unique features that make it easier to understand conceptually, making it a good starting point for understanding how to think about and leverage evolution. CHAPTERS 3 through 6 cover approaches to evolving proteins. CHAPTER 3 discusses the strengths and weaknesses of different approaches to the diversification of proteins, giving the reader a framework for designing libraries. CHAPTERS 4, 5, and 6 introduce different approaches to sorting by applying selection pressures to find the best variant from a large complex library. Upon completing this primer, the reader will have a theoretical basis to design and execute a directed evolution campaign. They will have the core knowledge to dive into the larger world of directed evolution and begin designing projects that leverage this powerful technique."-- $c Provided by publisher.
590 $a American Chemical Society, ACS In Focus eBooks - 2024 Front Files.
650 # 0 $a Nucleic acids $x Synthesis. $3 1483794
650 # 0 $a Proteins $x Synthesis. $3 996742
650 # 0 $a Enzymes $x Synthesis. $3 680879
650 # 0 $a Molecular evolution. $3 674535
650 # 0 $a Combinatorial chemistry. $3 562690
650 # 0 $a Evolution (Biology) $3 575821
650 # 7 $a SCIENCE / Biotechnology. $2 bisacsh $3 1483795
655 # 0 $a Electronic books. $2 local $3 554714
700 1 # $a Colee, Clair M., $e author. $u Scripps College. $3 1483793
710 2 # $a American Chemical Society. $3 1436935
830 0 $a ACS in focus, $x 2691-8307. $3 1437890
856 4 # $u https://dx.doi.org/10.1021/acsinfocus.7e8004