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CRISPR-Cas : = a laboratory manual /

Record Type:	Language materials, printed : Monograph/item
Title/Author:	CRISPR-Cas :/ edited by Jennifer Doudna, Prashant Mali.
Reminder of title:	a laboratory manual /
remainder title:	CRISPR Cas
other author:	Doudna, Jennifer A.
Published:	Cold Spring Harbor, NY :Cold Spring Harbor Laboratory Press, : c2016.,
Description:	x, 181 p. :ill. (chiefly col.) ; : 28 cm.;
Subject:	RNA - Laboratory manuals. - Analysis -
ISBN:	9781621821304

CRISPR-Cas : = a laboratory manual /
CRISPR-Cas :a laboratory manual /CRISPR Casedited by Jennifer Doudna, Prashant Mali. - Cold Spring Harbor, NY :Cold Spring Harbor Laboratory Press,c2016. - x, 181 p. :ill. (chiefly col.) ;28 cm.

Includes bibliographical references and index.

Overview of CRISPR-Cas9 Biology -- Guide RNAs: A Glimpse at the Sequences that Drive CRISPR-Cas Systems -- Characterization of Cas9-Guide RNA Orthologs -- Large-Scale Single Guide RNA Library Construction and Use for CRISPR-Cas9-Based Genetic Screens -- Adeno-Associated Virus-Mediated Delivery of CRISPR-Cas Systems for Genome Engineering in Mammalian Cells -- Detecting Single-Nucleotide Substitutions Induced by Genome Editing -- CRISPR- Cas9 Genome Engineering in Saccharomyces cerevisiae Cells -- Cas9-Mediated Genome Engineering in Drosophila melanogaster -- Optimization Strategies for the CRISPR- Cas9 Genome-Editing System -- Editing the Mouse Genome Using the CRISPR- Cas9 System -- Genome Editing in Human Pluripotent Stem Cells -- An Introduction to CRISPR Technology for Genome Activation and Repression in Mammalian Cells.

The development of CRISPR-Cas technology is revolutionizing biology. Based on machinery bacteria use to target foreign nucleic acids, these powerful techniques allow investigators to edit nucleic acids and modulate gene expression more rapidly and accurately than ever before.Featuring contributions from leading figures in the CRISPR-Cas field, this laboratory manual presents a state-of-the-art guide to the technology. It includes step-by-step protocols for applying CRISPR-Cas-based techniques in various systems, including yeast, zebrafish, Drosophila, mice, and cultured cells (e.g., human pluripotent stem cells). The contributors cover web-based tools and approaches for designing guide RNAs that precisely target genes of interest, methods for preparing and delivering CRISPR-Cas reagents into cells, and ways to screen for cells that harbor the desired genetic changes. Strategies for optimizing CRISPR-Cas in each system--especially for minimizing off-target effects--are also provided.Authors also describe other applications of the CRISPR-Cas system, including its use for regulating genome activation and repression, and discuss the development of next-generation CRISPR-Cas tools. The book is thus an essential laboratory resource for all cell, molecular, and developmental biologists, as well as biochemists, geneticists, and all who seek to expand their biotechnology toolkits.

ISBN: 9781621821304

LCCN: 2015050261

Nat. Bib. No.: GBB790043bnbSubjects--Topical Terms:

788530
RNA
--Analysis--Laboratory manuals.Index Terms--Genre/Form:

1335142
Laboratory Manual.

LC Class. No.: QP623 / .C75 2016

Dewey Class. No.: 572.8/8078

National Library of Medicine Call No.: 2016 D-798

CRISPR-Cas : = a laboratory manual /
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520 # $a The development of CRISPR-Cas technology is revolutionizing biology. Based on machinery bacteria use to target foreign nucleic acids, these powerful techniques allow investigators to edit nucleic acids and modulate gene expression more rapidly and accurately than ever before.Featuring contributions from leading figures in the CRISPR-Cas field, this laboratory manual presents a state-of-the-art guide to the technology. It includes step-by-step protocols for applying CRISPR-Cas-based techniques in various systems, including yeast, zebrafish, Drosophila, mice, and cultured cells (e.g., human pluripotent stem cells). The contributors cover web-based tools and approaches for designing guide RNAs that precisely target genes of interest, methods for preparing and delivering CRISPR-Cas reagents into cells, and ways to screen for cells that harbor the desired genetic changes. Strategies for optimizing CRISPR-Cas in each system--especially for minimizing off-target effects--are also provided.Authors also describe other applications of the CRISPR-Cas system, including its use for regulating genome activation and repression, and discuss the development of next-generation CRISPR-Cas tools. The book is thus an essential laboratory resource for all cell, molecular, and developmental biologists, as well as biochemists, geneticists, and all who seek to expand their biotechnology toolkits.
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