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Advanced methods in molecular biology and biotechnology = a practical lab manual /

紀錄類型:	書目-語言資料,印刷品 : Monograph/item
正題名/作者:	Advanced methods in molecular biology and biotechnology/ Khalid Z. Masoodi, Sameena Maqbool Lone and Rovidha Saba Rasool.
其他題名:	a practical lab manual /
作者:	Masoodi, Khalid Z.
其他作者:	Lone, Sameena Maqbool.
出版者:	London :Academic Press, : 2021.,
面頁冊數:	1 online resource (xxxi, 186 p.) :ill. :
標題:	Molecular biology - Congresses. -
電子資源:	https://www.sciencedirect.com/science/book/9780128244494
ISBN:	9780128244500

Advanced methods in molecular biology and biotechnology = a practical lab manual /
Masoodi, Khalid Z.

Advanced methods in molecular biology and biotechnologya practical lab manual /[electronic resource] :Khalid Z. Masoodi, Sameena Maqbool Lone and Rovidha Saba Rasool. - London :Academic Press,2021. - 1 online resource (xxxi, 186 p.) :ill.

Intro -- Advanced Methods in Molecular Biology and Biotechnology: A Practical Lab Manual -- Copyright -- Dedication -- Contents -- Preface -- Foreword -- Acknowledgment -- Introduction -- About the author -- Disclaimer -- Chapter 1 Introduction to molecular biology techniques -- Safety measures -- Preparation of the solutions -- Working with the nucleic acids -- Storage -- Purification -- Quantitation -- Concentration -- Restriction enzymes -- Sterile techniques -- References -- Chapter 2 Agarose gel electrophoresis -- Definition -- Rationale -- Before you begin -- Key resources table

Advanced Methods in Molecular Biology and Biotechnology: A Practical Lab Manual is a concise reference on common protocols and techniques for advanced molecular biology and biotechnology experimentation. Each chapter focuses on a different method, providing an overview before delving deeper into the procedure in a step-by-step approach. Techniques covered include genomic DNA extraction using cetyl trimethylammonium bromide (CTAB) and chloroform extraction, chromatographic techniques, ELISA, hybridization, gel electrophoresis, dot blot analysis and methods for studying polymerase chain reactions. Laboratory protocols and standard operating procedures for key equipment are also discussed, providing an instructive overview for lab work. This practical guide focuses on the latest advances and innovations in methods for molecular biology and biotechnology investigation, helping researchers and practitioners enhance and advance their own methodologies and take their work to the next level.

ISBN: 9780128244500Subjects--Topical Terms:

674960
Molecular biology
--Congresses.Index Terms--Genre/Form:

554714
Electronic books.

LC Class. No.: QH506

Dewey Class. No.: 572.80721

Advanced methods in molecular biology and biotechnology = a practical lab manual /
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505 0 $a Intro -- Advanced Methods in Molecular Biology and Biotechnology: A Practical Lab Manual -- Copyright -- Dedication -- Contents -- Preface -- Foreword -- Acknowledgment -- Introduction -- About the author -- Disclaimer -- Chapter 1 Introduction to molecular biology techniques -- Safety measures -- Preparation of the solutions -- Working with the nucleic acids -- Storage -- Purification -- Quantitation -- Concentration -- Restriction enzymes -- Sterile techniques -- References -- Chapter 2 Agarose gel electrophoresis -- Definition -- Rationale -- Before you begin -- Key resources table
505 8 $a Materials and equipments -- Materials and equipments required -- Reagents required -- Step-by-step method details -- Timing: 1-1.5 h (depending on the size of the sample) -- Expected outcomes -- Quantification and statistical analysis -- Advantages -- Limitations -- Optimization and troubleshooting -- Problem -- Potential solution -- Safety considerations and standards -- Alternative methods/procedures -- References -- Chapter 3 SDS-polyacrylamide gel electrophoresis -- Definition -- Rationale -- Before you begin -- Key resources table -- Materials and equipment -- Step-by-step method details
505 8 $a Timing: 3-4 h (depends on the plate size) -- Expected outcomes -- Quantification and statistical analysis -- Advantages -- Limitations -- Optimization and troubleshooting -- Problem -- Potential solution -- Safety considerations andstandards -- Alternative methods/procedures -- References -- Chapter 4 Immunoelectrophoresis -- Definition -- Before you begin -- Key resources table -- Materials and equipment -- Step-by-step method details -- Expected outcomes -- Advantages -- Limitations -- Optimization and troubleshooting -- Problem -- Potential solution -- Safety considerations and standards
505 8 $a Step-by-step method details -- Expected outcomes -- Advantages -- Limitations -- Optimization and troubleshooting -- Problem -- Potential solution -- Safety considerations and standards -- References -- Chapter 7 Genomic DNA extraction from the plant leaves using the CTAB method -- Definition -- Rationale -- Before you begin -- Key resources table -- Materials and equipment -- Step-by-step method details -- Expected outcomes -- Quantification and statistical analysis -- Advantages -- Limitations -- Optimization and troubleshooting -- Problem -- Potential solution.
520 $a Advanced Methods in Molecular Biology and Biotechnology: A Practical Lab Manual is a concise reference on common protocols and techniques for advanced molecular biology and biotechnology experimentation. Each chapter focuses on a different method, providing an overview before delving deeper into the procedure in a step-by-step approach. Techniques covered include genomic DNA extraction using cetyl trimethylammonium bromide (CTAB) and chloroform extraction, chromatographic techniques, ELISA, hybridization, gel electrophoresis, dot blot analysis and methods for studying polymerase chain reactions. Laboratory protocols and standard operating procedures for key equipment are also discussed, providing an instructive overview for lab work. This practical guide focuses on the latest advances and innovations in methods for molecular biology and biotechnology investigation, helping researchers and practitioners enhance and advance their own methodologies and take their work to the next level.
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