國立虎尾科技大學 |

A Study of the Synthesis of Cellulose by the Bacteria Komagataeibacter xylinus (K. Xylinus) when Under the Action of an Electric Field in a Microfluidic Reactor

Record Type:	Language materials, printed : Monograph/item
Title/Author:	A Study of the Synthesis of Cellulose by the Bacteria Komagataeibacter xylinus (K. Xylinus) when Under the Action of an Electric Field in a Microfluidic Reactor/ Sindora Reddy Baddam.
Author:	Baddam, Sindora Reddy,
Description:	1 electronic resource (91 pages)
Notes:	Source: Masters Abstracts International, Volume: 82-08.
Contained By:	Masters Abstracts International82-08.
Subject:	Biomedical engineering. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=28256702
ISBN:	9798569905522

A Study of the Synthesis of Cellulose by the Bacteria Komagataeibacter xylinus (K. Xylinus) when Under the Action of an Electric Field in a Microfluidic Reactor
Baddam, Sindora Reddy,

A Study of the Synthesis of Cellulose by the Bacteria Komagataeibacter xylinus (K. Xylinus) when Under the Action of an Electric Field in a Microfluidic Reactor [eletronic resource] /Sindora Reddy Baddam. - 1 electronic resource (91 pages)

Source: Masters Abstracts International, Volume: 82-08.

Dielectrophoresis (DEP) is an established technique used as a label-free method to manipulate microorganisms by exploiting the interaction of targeted microorganisms with a non-uniform electric field. The strength and direction of the DEP force on a targeted cell, specifically Komagataeibacter xylinus (K. xylinus), is dependent on the frequency of the applied electric field as well as the dielectric properties of the bacterial cell and suspending media.K. xylinus is a microorganism that can synthesize a fibrous, web-shaped organic material called bacterial cellulose (BC), which contains desirable properties such as high mechanical strength and increased water holding capabilities. By combining DEP techniques with this BC synthesizing microorganism, this creates the potential for controlled and enhanced growth allowing BC to be tailored to specific properties at specific locations. However, literature has reported limited information regarding the use of DEP techniques to study how electric fields affect BC synthesis in a microfluidic reactor.In this study, a titanium-based semicircular microelectrode chip was used to study how electrostimulation can potentially manipulate and influence the synthesis of BC at unique regions of interest where the electric field is greatest. This study features experiments running over fourteen days with an AC induced electric field at polarization voltages of 1 Vpp, 2 Vpp, and 5 Vpp at a frequency of 750 kHz. Results suggest that BC synthesis is possible and can be manipulated over an extended period as the polarization voltage increased where the electric field is greatest.

English

ISBN: 9798569905522Subjects--Topical Terms:

588770
Biomedical engineering.
Subjects--Index Terms:

Dielectrophoresis

A Study of the Synthesis of Cellulose by the Bacteria Komagataeibacter xylinus (K. Xylinus) when Under the Action of an Electric Field in a Microfluidic Reactor
LDR:03128nam a22003973i 4500 001 1172836
005 20260622113214.5
006 m o d
007 cr|nu||||||||
008 260803s2020 miu||||||m |||||||eng d
020 $a 9798569905522
035 $a (MiAaPQD)AAI28256702
035 $a AAI28256702
040 $a MiAaPQD $b eng $c MiAaPQD $e rda
100 1 $a Baddam, Sindora Reddy, $e author. $3 1503440
245 1 2 $a A Study of the Synthesis of Cellulose by the Bacteria Komagataeibacter xylinus (K. Xylinus) when Under the Action of an Electric Field in a Microfluidic Reactor $c Sindora Reddy Baddam. $h [eletronic resource] /
264 1 $a Ann Arbor : $b ProQuest Dissertations & Theses, $c 2020
300 $a 1 electronic resource (91 pages)
336 $a text $b txt $2 rdacontent
337 $a computer $b c $2 rdamedia
338 $a online resource $b cr $2 rdacarrier
500 $a Source: Masters Abstracts International, Volume: 82-08.
500 $a Advisors: Martinez-Duatre, Rodrigo Committee members: Xuan, Xianchung; Tallapragada, Phanindra.
502 $b M.S. $c Clemson University $d 2020.
520 # $a Dielectrophoresis (DEP) is an established technique used as a label-free method to manipulate microorganisms by exploiting the interaction of targeted microorganisms with a non-uniform electric field. The strength and direction of the DEP force on a targeted cell, specifically Komagataeibacter xylinus (K. xylinus), is dependent on the frequency of the applied electric field as well as the dielectric properties of the bacterial cell and suspending media.K. xylinus is a microorganism that can synthesize a fibrous, web-shaped organic material called bacterial cellulose (BC), which contains desirable properties such as high mechanical strength and increased water holding capabilities. By combining DEP techniques with this BC synthesizing microorganism, this creates the potential for controlled and enhanced growth allowing BC to be tailored to specific properties at specific locations. However, literature has reported limited information regarding the use of DEP techniques to study how electric fields affect BC synthesis in a microfluidic reactor.In this study, a titanium-based semicircular microelectrode chip was used to study how electrostimulation can potentially manipulate and influence the synthesis of BC at unique regions of interest where the electric field is greatest. This study features experiments running over fourteen days with an AC induced electric field at polarization voltages of 1 Vpp, 2 Vpp, and 5 Vpp at a frequency of 750 kHz. Results suggest that BC synthesis is possible and can be manipulated over an extended period as the polarization voltage increased where the electric field is greatest.
546 $a English
590 $a School code: 0050
650 # 4 $a Biomedical engineering. $3 588770
650 # 4 $a Mechanical engineering. $3 557493
653 # # $a Dielectrophoresis
653 # # $a Komagataeibacter xylinus
653 # # $a Bacterial cellulose
690 $a 0548
690 $a 0541
710 2 # $a Clemson University. $b Mechanical Engineering. $3 845453
720 1 $a Martinez-Duatre, Rodrigo $e degree supervisor.
773 0 # $t Masters Abstracts International $g 82-08.
790 $a 0050
791 $a M.S.
792 $a 2020
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=28256702