國立虎尾科技大學 |

Non-Linear Endoscopic Microscopy System for Potential use in Diagnosing Rheumatoid Arthritis.

紀錄類型:	書目-語言資料,印刷品 : Monograph/item
正題名/作者:	Non-Linear Endoscopic Microscopy System for Potential use in Diagnosing Rheumatoid Arthritis./
作者:	Tiwari, Nivedan.
面頁冊數:	112 p.
附註:	Source: Dissertation Abstracts International, Volume: 72-08, Section: B, page: 4948.
Contained By:	Dissertation Abstracts International72-08B.
標題:	Engineering, Biomedical. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3457315
ISBN:	9781124675862

Non-Linear Endoscopic Microscopy System for Potential use in Diagnosing Rheumatoid Arthritis.
Tiwari, Nivedan.

Non-Linear Endoscopic Microscopy System for Potential use in Diagnosing Rheumatoid Arthritis. - 112 p.

Source: Dissertation Abstracts International, Volume: 72-08, Section: B, page: 4948.

Thesis (Ph.D.)--University of California, Irvine, 2011.

Non-linear optical microscopy (NLOM) enables imaging three-dimensionally, deep inside tissue. These are capabilities that traditional two-dimensional as well as confocal microscopy lack. NLOM has been used extensively for imaging individual cells and tissues. This research focuses on the development of NLOM as a diagnostic technique with wide clinical applications. Toward this end, NLOM was first evaluated as a diagnostic tool for quantifying the progression of primary immune-mediated and non-immune inflammatory arthritis, using only the natural fluorescence properties of tissue to generate images. NLOM was performed on rabbit knee joint synovial samples using 730 nm and 800 nm excitation wavelengths. Less than 30 mW of excitation power delivered with a 40X, 0.8NA water immersion objective was sufficient for the visualization of synovial structures to a depth of 70 mum without tissue damage. NLOM imaging of normal and pathologic synovial tissue revealed the cellular structure, synoviocytes, adipocytes, collagen, vascular structures and differential characteristics of inflammatory infiltrates without requiring tissue processing or staining.

ISBN: 9781124675862Subjects--Topical Terms:

845403
Engineering, Biomedical.

Non-Linear Endoscopic Microscopy System for Potential use in Diagnosing Rheumatoid Arthritis.
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502 $a Thesis (Ph.D.)--University of California, Irvine, 2011.
520 $a Non-linear optical microscopy (NLOM) enables imaging three-dimensionally, deep inside tissue. These are capabilities that traditional two-dimensional as well as confocal microscopy lack. NLOM has been used extensively for imaging individual cells and tissues. This research focuses on the development of NLOM as a diagnostic technique with wide clinical applications. Toward this end, NLOM was first evaluated as a diagnostic tool for quantifying the progression of primary immune-mediated and non-immune inflammatory arthritis, using only the natural fluorescence properties of tissue to generate images. NLOM was performed on rabbit knee joint synovial samples using 730 nm and 800 nm excitation wavelengths. Less than 30 mW of excitation power delivered with a 40X, 0.8NA water immersion objective was sufficient for the visualization of synovial structures to a depth of 70 mum without tissue damage. NLOM imaging of normal and pathologic synovial tissue revealed the cellular structure, synoviocytes, adipocytes, collagen, vascular structures and differential characteristics of inflammatory infiltrates without requiring tissue processing or staining.
520 $a The data and experience obtained from the tissue study was used for the development of a new NLOM endoscopic system for minimally invasive diagnostic imaging of tissue with microscopic resolution. This system was designed for the generation and acquisition of two-photon fluorescence (TPF), second harmonic generation (SHG), and coherent anti-Stokes Raman spectroscopic (CARS) signals. The novel design of this rigid endoscope keeps the bulky scanning system outside the probe, thus allowing for the construction of endoscopes that are potentially a few hundred microns in diameter. The critical specifications of the endoscope are a 5mm diameter, 11 cm long GRIN lens with negligible axial chromatic aberration followed by a single element, high NA aspheric focusing lens that is used as an objective. The endoscopic imaging system, even without any optimization of the signal acquisition system, provided very good images of fluorescent beads, as well as of stained and unstained tissue. The ability of the prototype endoscope setup to perform CARS imaging of thick tissue in the epi direction is a novel addition to earlier work on CARS micro-endoscopy.
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