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Electrical coupling between micropat...

Clemson University.

Electrical coupling between micropatterned cardiomyocytes and stem cells.

Record Type:	Language materials, printed : Monograph/item
Title/Author:	Electrical coupling between micropatterned cardiomyocytes and stem cells./
Author:	Ma, Zhen.
Description:	158 p.
Notes:	Source: Dissertation Abstracts International, Volume: 73-05, Section: B, page: .
Contained By:	Dissertation Abstracts International73-05B.
Subject:	Engineering, Biomedical. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3491194
ISBN:	9781267130846

Electrical coupling between micropatterned cardiomyocytes and stem cells.
Ma, Zhen.

Electrical coupling between micropatterned cardiomyocytes and stem cells. - 158 p.

Source: Dissertation Abstracts International, Volume: 73-05, Section: B, page: .

Thesis (Ph.D.)--Clemson University, 2011.

To understand how stem cells functionally couple with native cardiomyocytes is crucial for cell-based therapies to restore the loss of cardiomyocytes that occurs during heart infarction and other cardiac diseases. Due to the complexity of the in vivo environment, our knowledge of cell coupling is heavily dependent on cell-culture models. However, conventional in vitro studies involve undefined cell shapes and random length of cell-cell contacts in addition to the presence of multiple homotypic and heterotypic contacts between interacting cells. Thus, it has not been feasible to study electrical coupling corresponding to isolated specific types of cell contact modes.

ISBN: 9781267130846Subjects--Topical Terms:

845403
Engineering, Biomedical.

Electrical coupling between micropatterned cardiomyocytes and stem cells.
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020 $a 9781267130846
035 $a (UMI)AAI3491194
035 $a AAI3491194
040 $a UMI $c UMI
100 1 $a Ma, Zhen. $3 845527
245 1 0 $a Electrical coupling between micropatterned cardiomyocytes and stem cells.
300 $a 158 p.
500 $a Source: Dissertation Abstracts International, Volume: 73-05, Section: B, page: .
500 $a Adviser: Bruce Zhi Gao.
502 $a Thesis (Ph.D.)--Clemson University, 2011.
520 $a To understand how stem cells functionally couple with native cardiomyocytes is crucial for cell-based therapies to restore the loss of cardiomyocytes that occurs during heart infarction and other cardiac diseases. Due to the complexity of the in vivo environment, our knowledge of cell coupling is heavily dependent on cell-culture models. However, conventional in vitro studies involve undefined cell shapes and random length of cell-cell contacts in addition to the presence of multiple homotypic and heterotypic contacts between interacting cells. Thus, it has not been feasible to study electrical coupling corresponding to isolated specific types of cell contact modes.
520 $a To address this issue, we used microfabrication techniques to develop different geometrically-defined stem cell-cardiomyocyte contact assays to comparatively and quantitatively study functional stem cell-cardiomyocyte electrical coupling. Through geometric confinements, we will construct a matrix of identical microwells, and each was constructed as a specific microenvironment. Using laser-guided cell micropatterning technique, individual stem cells or cardiomyocytes can be deposited into the microwells to form certain contact mode. In this research, we firstly constructed an in-vivo like cardiac muscle fiber microenvironment, and the electrical conductivity of stem cells was investigated by inserting stem cells as cellular bridges. Then, the electrical coupling between cardiomyocytes and stem cells was studied at single-cell level by constructing contact-promotive/-preventive microenvironments.
590 $a School code: 0050.
650 4 $a Engineering, Biomedical. $3 845403
650 4 $a Physics, Optics. $3 845404
650 4 $a Biophysics, General. $3 845421
690 $a 0541
690 $a 0752
690 $a 0786
710 2 $a Clemson University. $b Bioengineering. $3 845528
773 0 $t Dissertation Abstracts International $g 73-05B.
790 1 0 $a Gao, Bruce Zhi, $e advisor
790 1 0 $a Borg, Thomas Keith $e committee member
790 1 0 $a Simionescu, Dan $e committee member
790 1 0 $a Dean, Delphine $e committee member
790 $a 0050
791 $a Ph.D.
792 $a 2011
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3491194

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