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Rapid cell magnetisation using catio...
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SpringerLink (Online service)
Rapid cell magnetisation using cationised magnetoferritin
Record Type:
Language materials, printed : Monograph/item
Title/Author:
Rapid cell magnetisation using cationised magnetoferritin/ by Sara Correia Carreira.
Author:
Carreira, Sara Correia.
Published:
Cham :Springer International Publishing : : 2017.,
Description:
xvii, 166 p. :ill., digital ; : 24 cm.;
Contained By:
Springer eBooks
Subject:
Biophysics. -
Online resource:
http://dx.doi.org/10.1007/978-3-319-60333-9
ISBN:
9783319603339
Rapid cell magnetisation using cationised magnetoferritin
Carreira, Sara Correia.
Rapid cell magnetisation using cationised magnetoferritin
[electronic resource] /by Sara Correia Carreira. - Cham :Springer International Publishing :2017. - xvii, 166 p. :ill., digital ;24 cm. - Springer theses,2190-5053. - Springer theses..
Introduction -- Materials and Methods -- Characterising Magnetoferritin and Cationised Magnetoferritin -- Stem Cell Labelling with Cationised Magnetoferritin -- Toxicological Profiling of Cationised Magnetoferritin -- Escherichia coli labelling with Cationised Magnetoferritin -- Overall Conclusions and Future Opportunities.
Bringing together nanoscience with stem cell and bacterial cell biology, this thesis is truly interdisciplinary in scope. It shows that the creation of superparamagnetic nanoparticles inside a protein coat, followed by chemical functionalisation of the protein surface, provides a novel methodology for cell magnetisation using incubation times as short as one minute. Crucially, stem cell proliferation and multi-lineage differentiation capacity is not impaired after labelling. Due to the unspecific labelling mechanism, this thesis also shows that the same magnetic protein nanoparticles can be used for rapid bacterial magnetisation. Thus, it is possible to magnetically capture and concentrate pathogens from clinical samples quickly and highly efficiently.
ISBN: 9783319603339
Standard No.: 10.1007/978-3-319-60333-9doiSubjects--Topical Terms:
581576
Biophysics.
LC Class. No.: QH505
Dewey Class. No.: 571.47
Rapid cell magnetisation using cationised magnetoferritin
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Introduction -- Materials and Methods -- Characterising Magnetoferritin and Cationised Magnetoferritin -- Stem Cell Labelling with Cationised Magnetoferritin -- Toxicological Profiling of Cationised Magnetoferritin -- Escherichia coli labelling with Cationised Magnetoferritin -- Overall Conclusions and Future Opportunities.
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Bringing together nanoscience with stem cell and bacterial cell biology, this thesis is truly interdisciplinary in scope. It shows that the creation of superparamagnetic nanoparticles inside a protein coat, followed by chemical functionalisation of the protein surface, provides a novel methodology for cell magnetisation using incubation times as short as one minute. Crucially, stem cell proliferation and multi-lineage differentiation capacity is not impaired after labelling. Due to the unspecific labelling mechanism, this thesis also shows that the same magnetic protein nanoparticles can be used for rapid bacterial magnetisation. Thus, it is possible to magnetically capture and concentrate pathogens from clinical samples quickly and highly efficiently.
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Physics and Astronomy (Springer-11651)
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