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The Porcine Memory B Cell in Conferring Long Term Adaptive Immunity to Viral Pathogens.

Record Type:	Language materials, manuscript : Monograph/item
Title/Author:	The Porcine Memory B Cell in Conferring Long Term Adaptive Immunity to Viral Pathogens./
Author:	Rahe, Michael Christopher.
Description:	1 online resource (164 pages)
Notes:	Source: Dissertation Abstracts International, Volume: 79-02(E), Section: B.
Contained By:	Dissertation Abstracts International79-02B(E).
Subject:	Immunology. -
Online resource:	click for full text (PQDT)
ISBN:	9780355405989

The Porcine Memory B Cell in Conferring Long Term Adaptive Immunity to Viral Pathogens.
Rahe, Michael Christopher.

The Porcine Memory B Cell in Conferring Long Term Adaptive Immunity to Viral Pathogens. - 1 online resource (164 pages)

Source: Dissertation Abstracts International, Volume: 79-02(E), Section: B.

Thesis (Ph.D.)

Includes bibliographical references

Porcine reproductive and respiratory syndrome virus (PRRSV) is the most important pathogen of swine health and well-being worldwide. Discovered nearly thirty years ago, there is still no vaccine capable of producing a broadly protective immune response against the virus. This deficiency is due in large part to a failure to understand how the adaptive immune system responds to vaccination or infection. Specifically, there is little to no knowledge regarding the all-important memory immune response to PRRSV. The objective of this dissertation was to fill in this significant gap in knowledge by identifying and characterizing the memory B cell response to PRRSV vaccination. First, we identified the presence of memory B cells against PRRSV non-structural protein 7 (nsp7) through the use of a novel in vitro B cell culture system. Next, we created and validated a novel reagent, a B cell tetramer, against nsp7 to enhance the speed and sensitivity of memory cell identification. Finally, through the utilization of the nsp7 tetramer, we evaluated the regional specificity and dynamic nsp7 specific B cell response to PRRSV MLV vaccination within select secondary lymphoid organs. These results constitute the first evidence of regional specialization of the B cell response to vaccination in an outbred animal species. Furthermore, the presented methods are a blueprint for the study of antigen specific cellular responses to any significant pathogen of animals important for food or fiber.

Electronic reproduction.
Ann Arbor, Mich. :
ProQuest,
2018

Mode of access: World Wide Web

ISBN: 9780355405989Subjects--Topical Terms:

592892
Immunology.
Index Terms--Genre/Form:

554714
Electronic books.

The Porcine Memory B Cell in Conferring Long Term Adaptive Immunity to Viral Pathogens.
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100 1 $a Rahe, Michael Christopher. $3 1179749
245 1 4 $a The Porcine Memory B Cell in Conferring Long Term Adaptive Immunity to Viral Pathogens.
264 0 $c 2017
300 $a 1 online resource (164 pages)
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500 $a Source: Dissertation Abstracts International, Volume: 79-02(E), Section: B.
500 $a Adviser: Michael P. Murtaugh.
502 $a Thesis (Ph.D.) $c University of Minnesota $d 2017.
504 $a Includes bibliographical references
520 $a Porcine reproductive and respiratory syndrome virus (PRRSV) is the most important pathogen of swine health and well-being worldwide. Discovered nearly thirty years ago, there is still no vaccine capable of producing a broadly protective immune response against the virus. This deficiency is due in large part to a failure to understand how the adaptive immune system responds to vaccination or infection. Specifically, there is little to no knowledge regarding the all-important memory immune response to PRRSV. The objective of this dissertation was to fill in this significant gap in knowledge by identifying and characterizing the memory B cell response to PRRSV vaccination. First, we identified the presence of memory B cells against PRRSV non-structural protein 7 (nsp7) through the use of a novel in vitro B cell culture system. Next, we created and validated a novel reagent, a B cell tetramer, against nsp7 to enhance the speed and sensitivity of memory cell identification. Finally, through the utilization of the nsp7 tetramer, we evaluated the regional specificity and dynamic nsp7 specific B cell response to PRRSV MLV vaccination within select secondary lymphoid organs. These results constitute the first evidence of regional specialization of the B cell response to vaccination in an outbred animal species. Furthermore, the presented methods are a blueprint for the study of antigen specific cellular responses to any significant pathogen of animals important for food or fiber.
533 $a Electronic reproduction. $b Ann Arbor, Mich. : $c ProQuest, $d 2018
538 $a Mode of access: World Wide Web
650 4 $a Immunology. $3 592892
650 4 $a Veterinary science. $3 1179701
655 7 $a Electronic books. $2 local $3 554714
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690 $a 0778
710 2 $a ProQuest Information and Learning Co. $3 1178819
710 2 $a University of Minnesota. $b Comparative and Molecular Biosciences. $3 1179750
773 0 $t Dissertation Abstracts International $g 79-02B(E).
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=10600971 $z click for full text (PQDT)