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Intrinsic interactions between noble metal nanoparticles and biomolecules : = Fundamental study and analytical applications.

紀錄類型:	書目-語言資料,手稿 : Monograph/item
正題名/作者:	Intrinsic interactions between noble metal nanoparticles and biomolecules :/
其他題名:	Fundamental study and analytical applications.
作者:	Yu, Lu.
面頁冊數:	1 online resource (133 pages)
附註:	Source: Dissertation Abstracts International, Volume: 78-03(E), Section: B.
Contained By:	Dissertation Abstracts International78-03B(E).
標題:	Mechanical engineering. -
電子資源:	click for full text (PQDT)
ISBN:	9781369099416

Intrinsic interactions between noble metal nanoparticles and biomolecules : = Fundamental study and analytical applications.
Yu, Lu.

Intrinsic interactions between noble metal nanoparticles and biomolecules :Fundamental study and analytical applications. - 1 online resource (133 pages)

Source: Dissertation Abstracts International, Volume: 78-03(E), Section: B.

Thesis (Ph.D.)

Includes bibliographical references

The focus of this dissertation is to explore the intrinsic interactions between noble metal nanoparticles (NMNPs) and biomolecules for various analytical applications. Biomolecules with amine groups and nitrogen atoms on cyclic rings could have strong noncovalent interactions with NMNPs. By displacing the charged capping agents on NMNPs, surface adsorption of biomolecules could significantly affect the stability of NMNPs. By utilizing the change of stability of NMNPs upon adsorption of biomolecules, four analytical applications have been developed in this dissertation.

Electronic reproduction.
Ann Arbor, Mich. :
ProQuest,
2018

Mode of access: World Wide Web

ISBN: 9781369099416Subjects--Topical Terms:

557493
Mechanical engineering.
Index Terms--Genre/Form:

554714
Electronic books.

Intrinsic interactions between noble metal nanoparticles and biomolecules : = Fundamental study and analytical applications.
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245 1 0 $a Intrinsic interactions between noble metal nanoparticles and biomolecules : $b Fundamental study and analytical applications.
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500 $a Source: Dissertation Abstracts International, Volume: 78-03(E), Section: B.
500 $a Adviser: Na Li.
502 $a Thesis (Ph.D.) $c University of Miami $d 2016.
504 $a Includes bibliographical references
520 $a The focus of this dissertation is to explore the intrinsic interactions between noble metal nanoparticles (NMNPs) and biomolecules for various analytical applications. Biomolecules with amine groups and nitrogen atoms on cyclic rings could have strong noncovalent interactions with NMNPs. By displacing the charged capping agents on NMNPs, surface adsorption of biomolecules could significantly affect the stability of NMNPs. By utilizing the change of stability of NMNPs upon adsorption of biomolecules, four analytical applications have been developed in this dissertation.
520 $a Firstly, a critical coagulation concentration (CCC)-based salt titration was developed to enable visual quantification in NMNP-based colorimetric detection (Chapter 2 and 3). Visual quantification using this CCC-based salt titration has been demonstrated in melamine and DNA detection, with a sensitivity and reliability comparable or higher than that using an absorbance reader. With no need for any analytical equipment, this visual quantification method is highly suitable for use in low resource settings.
520 $a Secondly, a CCC-based colorimetric assay was developed for studying the interaction strengths between NMNPs and oligonucleotides/nucleotides (Chapter 4). This assay has been used to determine the interaction strengths between various NMNPs and DNA/RNA oligonucleotides/nucleotides. The results showed that the interaction strengths of 15 nt DNA homo-oligonucleotides with silver nanoparticles (AgNPs) ranks as A>C>T while that with gold nanoparticles (AuNPs) ranks as A>T>C. Interaction strengths of DNA monophosphate and triple phosphate nucleotides with AuNPs ranks as G>T>A>C while interaction strengths of RNA monophosphate nucleotides with AuNPs ranks as on AgNPs of G>U>A>C.
520 $a Thirdly, a colorimetric method was developed for determining the interaction strengths between NMNPs and nucleobases/nucleosides (Chapter 5). This assay has been used to determine the interaction strengths between AgNPs and various DNA/RNA nucleobases/nucleosides. Interaction strengths with AgNPs ranks as A>U>T>C>G for nucleobases, G>A>T>C for DNA nucleosides, and A>G>U>C for RNA nucleosides.
520 $a Lastly, a NMNP-bases method was developed to determine the net electric charges of antibiotics (Chapter 6). This method has been demonstrated with vancomycin. The charge of vancomycin at near neutral pH determined with this method was comparable to that reported in the literature. With a high sensitivity and general applicability, this NNMP-based method could be an appealing alternative for existing charge determination methods.
533 $a Electronic reproduction. $b Ann Arbor, Mich. : $c ProQuest, $d 2018
538 $a Mode of access: World Wide Web
650 4 $a Mechanical engineering. $3 557493
650 4 $a Biomedical engineering. $3 588770
650 4 $a Nanotechnology. $3 557660
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710 2 $a University of Miami. $b Mechanical Engineering (Engineering). $3 1180258
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856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=10154850 $z click for full text (PQDT)