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Modification of poly(methyl methacrylate) surfaces with azobenzene groups to develop a photoresponsive surface.

Record Type:	Language materials, manuscript : Monograph/item
Title/Author:	Modification of poly(methyl methacrylate) surfaces with azobenzene groups to develop a photoresponsive surface./
Author:	Clarke, Ashley Elizabeth.
Description:	1 online resource (115 pages)
Notes:	Source: Dissertation Abstracts International, Volume: 75-12C.
Contained By:	Dissertation Abstracts International75-12C.
Subject:	Chemical engineering. -
Online resource:	click for full text (PQDT)

Modification of poly(methyl methacrylate) surfaces with azobenzene groups to develop a photoresponsive surface.
Clarke, Ashley Elizabeth.

Modification of poly(methyl methacrylate) surfaces with azobenzene groups to develop a photoresponsive surface. - 1 online resource (115 pages)

Source: Dissertation Abstracts International, Volume: 75-12C.

Thesis (M.A.Sc.)

Includes bibliographical references

Photoswitchable surfaces can be used to reversibly control surface wettability, adsorptivity, and protein adhesion to influence cell interactions at the surface. Control over protein adsorption and cell adhesion is useful in many applications, from cell culture scaffolds to drug delivery devices and improving the cell response to materials in vivo. Switchable properties can be attained by polymerizing light-responsive monomers or by modifying existing materials. This project investigates surface modification as a method of developing light-responsive poly(methyl methacrylate) (PMMA) surfaces and coatings of a copolymer of methyl methacrylate (MMA) and 2-aminoethyl methacrylate (AEM) (termed MMAcoAEM).

Electronic reproduction.
Ann Arbor, Mich. :
ProQuest,
2018

Mode of access: World Wide Web

Subjects--Topical Terms:

555952
Chemical engineering.
Index Terms--Genre/Form:

554714
Electronic books.

Modification of poly(methyl methacrylate) surfaces with azobenzene groups to develop a photoresponsive surface.
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245 1 0 $a Modification of poly(methyl methacrylate) surfaces with azobenzene groups to develop a photoresponsive surface.
264 0 $c 2018
300 $a 1 online resource (115 pages)
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500 $a Source: Dissertation Abstracts International, Volume: 75-12C.
500 $a Adviser: Laura Wells.
502 $a Thesis (M.A.Sc.) $c Queen's University (Canada) $d 2018.
504 $a Includes bibliographical references
520 $a Photoswitchable surfaces can be used to reversibly control surface wettability, adsorptivity, and protein adhesion to influence cell interactions at the surface. Control over protein adsorption and cell adhesion is useful in many applications, from cell culture scaffolds to drug delivery devices and improving the cell response to materials in vivo. Switchable properties can be attained by polymerizing light-responsive monomers or by modifying existing materials. This project investigates surface modification as a method of developing light-responsive poly(methyl methacrylate) (PMMA) surfaces and coatings of a copolymer of methyl methacrylate (MMA) and 2-aminoethyl methacrylate (AEM) (termed MMAcoAEM).
520 $a In this work, PMMA surfaces were functionalized with amine or carboxyl groups for azobenzene modification and cell-material interaction studies. PMMA was functionalized with hexamethylenediamine (HMD) or O,O'-bis(2-aminopropyl) propylene glycol-block-polyethylene glycol-block-polypropylene glycol (PPG-PEG-PPG) groups via aminolysis or functionalized with carboxyl groups via hydrolysis. Aminated PMMA was then modified with 4-(phenylazo)benzoic acid using a carbodiimide reaction. Surfaces were characterized using ninhydrin assays, titration, contact angle measurements, x-ray photoelectron spectroscopy (XPS), ultraviolet-visible (UV-VIS) spectroscopy and nuclear magnetic resonance spectroscopy (NMR).
520 $a The aminolysis reaction conditions from literature were revised to improve the graft density, with up to 36.4 +/- 7.0 nmol/cm2 of HMD or 20.3 +/- 2.9 nmol/cm2 of PPG-PEG-PPG groups functionalized to PMMA. The copolymer MMAcoAEM contained 44.0 +/- 2.2 nmol/cm2 of amine groups as a surface coating. Amine-functionalized PMMA or MMAcoAEM surfaces were modified with 20.5 +/- 0.4 nmol/cm2 (HMD), 11.2 +/- 2.6 nmol/cm2 (PPG-PEG-PPG), or 24.8 +/- 4.4 nmol/cm2 (MMAcoAEM) of 4-(phenylazo)benzoic acid. UV-VIS spectroscopy was used to confirm that azobenzene grafted to materials retained the ability to photoisomerize and interact with beta-cyclodextrin (beta-CD).
520 $a Functionalized PMMA was also used to study cell-material interactions with neutrophil-like HL-60 human promyelocytic leukemia cells activated with phorbol 12-myristate 13-acetate (PMA) to induce cell adhesion. AlamarBlue assays and live/dead deoxyribonucleic acid (DNA) staining indicated that amine-modified surfaces contained the highest amount of extracellular DNA after incubation with HL-60s, believed to be extracellular traps (ETs). Future research aims to study the cell-material interactions with azobenzene-modified surfaces and further modify the surface using biomolecules conjugated to beta-CD.
533 $a Electronic reproduction. $b Ann Arbor, Mich. : $c ProQuest, $d 2018
538 $a Mode of access: World Wide Web
650 4 $a Chemical engineering. $3 555952
655 7 $a Electronic books. $2 local $3 554714
690 $a 0542
710 2 $a ProQuest Information and Learning Co. $3 1178819
710 2 $a Queen's University (Canada). $3 1148613
773 0 $t Dissertation Abstracts International $g 75-12C.
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=10760616 $z click for full text (PQDT)