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Structural Basis for RNA editing and site selectivity by ADAR2.

Record Type:	Language materials, manuscript : Monograph/item
Title/Author:	Structural Basis for RNA editing and site selectivity by ADAR2./
Author:	Thomas, Justin McIntyre.
Description:	1 online resource (127 pages)
Notes:	Source: Dissertation Abstracts International, Volume: 79-05(E), Section: B.
Contained By:	Dissertation Abstracts International79-05B(E).
Subject:	Chemistry. -
Online resource:	click for full text (PQDT)
ISBN:	9780355462111

Structural Basis for RNA editing and site selectivity by ADAR2.
Thomas, Justin McIntyre.

Structural Basis for RNA editing and site selectivity by ADAR2. - 1 online resource (127 pages)

Source: Dissertation Abstracts International, Volume: 79-05(E), Section: B.

Thesis (Ph.D.)

Includes bibliographical references

Adenosine deaminases acting on RNA (ADARs) are RNA editing enzymes that convert adenosine to inosine in duplex RNA. Because inosine behaves like guanosine in Watson-Crick base pairing, A-to-I editing may have wide ranging consequences in RNA function. The X-ray crystal structure of the deaminase domain of one member of the ADAR family (ADAR2) was solved over a decade ago, however this structure lacked RNA and provided limited information on how ADARs select, bind and edit adenosines within duplex RNA. This dissertation describes solving of X-ray co-crystal structures of ADAR2's deaminase domain bound to synthetic duplex RNA and subsequent experiments to define the structural basis of the enzymes sequence preferences.

Electronic reproduction.
Ann Arbor, Mich. :
ProQuest,
2018

Mode of access: World Wide Web

ISBN: 9780355462111Subjects--Topical Terms:

593913
Chemistry.
Index Terms--Genre/Form:

554714
Electronic books.

Structural Basis for RNA editing and site selectivity by ADAR2.
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100 1 $a Thomas, Justin McIntyre. $3 1182772
245 1 0 $a Structural Basis for RNA editing and site selectivity by ADAR2.
264 0 $c 2017
300 $a 1 online resource (127 pages)
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500 $a Source: Dissertation Abstracts International, Volume: 79-05(E), Section: B.
500 $a Adviser: Peter A. Beal.
502 $a Thesis (Ph.D.) $c University of California, Davis $d 2017.
504 $a Includes bibliographical references
520 $a Adenosine deaminases acting on RNA (ADARs) are RNA editing enzymes that convert adenosine to inosine in duplex RNA. Because inosine behaves like guanosine in Watson-Crick base pairing, A-to-I editing may have wide ranging consequences in RNA function. The X-ray crystal structure of the deaminase domain of one member of the ADAR family (ADAR2) was solved over a decade ago, however this structure lacked RNA and provided limited information on how ADARs select, bind and edit adenosines within duplex RNA. This dissertation describes solving of X-ray co-crystal structures of ADAR2's deaminase domain bound to synthetic duplex RNA and subsequent experiments to define the structural basis of the enzymes sequence preferences.
520 $a Chapter 2 describes the solved crystal structures of ADAR2 deaminase domain-RNA complexes. The deaminase domain's RNA binding interface is analyzed and new RNA binding residues are identified. In Chapter 3 the importance of newly identified RNA binding residues is examined through in-vitro biochemical assays. The structural basis for ADAR2's nearest neighbor sequence preferences are also defined by probing contacts made to the RNA using chemically modified RNA substrates. This information may aid the development of systems to direct A-to-I editing of specific adenosines using ADARs.
520 $a In Chapter 4, RNA binding experiments using ADAR2 constructs bearing double stranded RNA binding domains (dsRBDs) are carried out with the goal of better understanding how the dsRBDs affect the specificity and behavior of the full length ADAR2. Mobility shift assays, RNA cleavage footprinting assays and electron microscopy all provide evidence that ADAR2 undergoes a specific RNA dependent dimerization. Finally, efforts to obtain high resolution structural data for ADAR2 constructs bearing dsRBDs through X-ray crystallography and Cryo-electron microscopy are discussed.
533 $a Electronic reproduction. $b Ann Arbor, Mich. : $c ProQuest, $d 2018
538 $a Mode of access: World Wide Web
650 4 $a Chemistry. $3 593913
650 4 $a Biochemistry. $3 582831
655 7 $a Electronic books. $2 local $3 554714
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690 $a 0487
710 2 $a ProQuest Information and Learning Co. $3 1178819
710 2 $a University of California, Davis. $b Chemistry. $3 1182773
773 0 $t Dissertation Abstracts International $g 79-05B(E).
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