國立虎尾科技大學 |

Dynamic Control of DNA Precursor Synthesis in Early Embryos.

Record Type:	Language materials, manuscript : Monograph/item
Title/Author:	Dynamic Control of DNA Precursor Synthesis in Early Embryos./
Author:	Song, Yonghyun.
Description:	1 online resource (70 pages)
Notes:	Source: Dissertation Abstracts International, Volume: 79-05(E), Section: B.
Contained By:	Dissertation Abstracts International79-05B(E).
Subject:	Molecular biology. -
Online resource:	click for full text (PQDT)
ISBN:	9780355480658

Dynamic Control of DNA Precursor Synthesis in Early Embryos.
Song, Yonghyun.

Dynamic Control of DNA Precursor Synthesis in Early Embryos. - 1 online resource (70 pages)

Source: Dissertation Abstracts International, Volume: 79-05(E), Section: B.

Thesis (Ph.D.)

Includes bibliographical references

Animal embryogenesis starts with multiple rounds of nuclear divisions. During and shortly after these divisions, the zygotic genome is activated, the body plan of the organism is established, and gastrulation initiates the formation of tissues and organs. For these events to occur, the embryo needs to generate energy and provide metabolic precursors for biosynthesis. In this thesis, we used quantitative mass spectrometry and genetic manipulation techniques to examine how the early Drosophila melanogaster embryo controls the synthesis of DNA precursors.

Electronic reproduction.
Ann Arbor, Mich. :
ProQuest,
2018

Mode of access: World Wide Web

ISBN: 9780355480658Subjects--Topical Terms:

583443
Molecular biology.
Index Terms--Genre/Form:

554714
Electronic books.

Dynamic Control of DNA Precursor Synthesis in Early Embryos.
LDR:03163ntm a2200373Ki 4500 001 911139
005 20180517121920.5
006 m o u
007 cr mn||||a|a||
008 190606s2017 xx obm 000 0 eng d
020 $a 9780355480658
035 $a (MiAaPQ)AAI10639104
035 $a (MiAaPQ)princeton:12371
035 $a AAI10639104
040 $a MiAaPQ $b eng $c MiAaPQ
099 $a TUL $f hyy $c available through World Wide Web
100 1 $a Song, Yonghyun. $3 1182796
245 1 0 $a Dynamic Control of DNA Precursor Synthesis in Early Embryos.
264 0 $c 2017
300 $a 1 online resource (70 pages)
336 $a text $b txt $2 rdacontent
337 $a computer $b c $2 rdamedia
338 $a online resource $b cr $2 rdacarrier
500 $a Source: Dissertation Abstracts International, Volume: 79-05(E), Section: B.
500 $a Adviser: Stanislav Y. Shvartsman.
502 $a Thesis (Ph.D.) $c Princeton University $d 2017.
504 $a Includes bibliographical references
520 $a Animal embryogenesis starts with multiple rounds of nuclear divisions. During and shortly after these divisions, the zygotic genome is activated, the body plan of the organism is established, and gastrulation initiates the formation of tissues and organs. For these events to occur, the embryo needs to generate energy and provide metabolic precursors for biosynthesis. In this thesis, we used quantitative mass spectrometry and genetic manipulation techniques to examine how the early Drosophila melanogaster embryo controls the synthesis of DNA precursors.
520 $a Early Drosophila embryos undergo 13 rapid and synchronous nuclear division cycles within two hours of fertilization. This exponential increase in the number of nuclei requires massive amounts of deoxynucleoside triphosphates (dNTPs). Surprisingly, despite the breakneck speed at which Drosophila embryos synthesize DNA, maternally deposited dNTPs can generate less than half of the genomes needed to reach gastrulation. The rest of the dNTPs are synthesized "on the go". The rate-limiting enzyme of dNTP synthesis, ribonucleotide reductase (RNR), is inhibited by endogenous levels of dATP present at fertilization and is activated as dATP is depleted via DNA polymerization. In the absence of inhibition by dATP, dNTP levels increase dramatically and induce embryonic lethality with particularly severe structural defects in the anterior regions.
520 $a In conclusion, this thesis demonstrated that dNTP synthesis in early Drosophila embryos is controlled mainly through a single feedback inhibition loop at the end of the dNTP production pathway. In the process, we have also found that misregulation of RNR activity surprisingly confers tissue specific defects. Going forward, this thesis establishes Drosophila development as a platform for mechanistic and quantitative studies of dNTP metabolism.
533 $a Electronic reproduction. $b Ann Arbor, Mich. : $c ProQuest, $d 2018
538 $a Mode of access: World Wide Web
650 4 $a Molecular biology. $3 583443
650 4 $a Biochemistry. $3 582831
655 7 $a Electronic books. $2 local $3 554714
690 $a 0307
690 $a 0487
710 2 $a ProQuest Information and Learning Co. $3 1178819
710 2 $a Princeton University. $b Quantitative Computational Biology. $3 1182797
773 0 $t Dissertation Abstracts International $g 79-05B(E).
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=10639104 $z click for full text (PQDT)