國立虎尾科技大學 |

Export and Role of Flavin Electron Shuttles in Shewanella oneidensis Strain MR-1.

Record Type:	Language materials, manuscript : Monograph/item
Title/Author:	Export and Role of Flavin Electron Shuttles in Shewanella oneidensis Strain MR-1./
Author:	Kotloski, Nicholas Jason.
Description:	1 online resource (130 pages)
Notes:	Source: Dissertation Abstracts International, Volume: 76-03(E), Section: B.
Contained By:	Dissertation Abstracts International76-03B(E).
Subject:	Microbiology. -
Online resource:	click for full text (PQDT)
ISBN:	9781321343199

Export and Role of Flavin Electron Shuttles in Shewanella oneidensis Strain MR-1.
Kotloski, Nicholas Jason.

Export and Role of Flavin Electron Shuttles in Shewanella oneidensis Strain MR-1. - 1 online resource (130 pages)

Source: Dissertation Abstracts International, Volume: 76-03(E), Section: B.

Thesis (Ph.D.)

Includes bibliographical references

Our planet is home to microbes with an impressive diversity of metabolic capabilities. For instance, dissimilatory metal reducing bacteria (DMRB) can respire minerals, heavy metals, and electrodes. Electrode respiration is important both for investigating DMRB and biotechnology applications including biosensors, bioenergy, and wastewater treatment. Placing electrodes in wastewater allows for electricity production and oxidation of carbon sources to carbon dioxide. Shewanella oneidensis MR-1, a model DMRB, transfers electrons generated from metabolism across the inner and outer membranes to insoluble external electron acceptors. This feat is accomplished using an electron conduit consisting of multi-heme cytochromes and flavin electron shuttles. The electron conduit in Shewanella evolved to move electrons out of the cell, but in controlled environments, the conduit is functionally reversible, meaning electrons can flow either out of or into the cell. In a process termed electrosynthesis, electrons are transferred from an electrode into a cell to direct metabolism to produce specific metabolites. A useful way to study electrosynthesis in cells is to use three-electrode bioreactors. The working electrode in a bioreactor is poised at an oxidizing or reducing potential and continuously measures electrons entering or leaving the electrode. When electrodes were used to donate electrons to attached Shewanella cells, intracellular ATP was produced. Mutants defective in generating or using proton motive force were unable to produce wild-type levels of ATP. If significant quantities of ATP can be produced by reverse electron flow, it will be a critical step towards fixing carbon dioxide into metabolic precursors and eventually into biofuels.

Electronic reproduction.
Ann Arbor, Mich. :
ProQuest,
2018

Mode of access: World Wide Web

ISBN: 9781321343199Subjects--Topical Terms:

591510
Microbiology.
Index Terms--Genre/Form:

554714
Electronic books.

Export and Role of Flavin Electron Shuttles in Shewanella oneidensis Strain MR-1.
LDR:03027ntm a2200337Ki 4500 001 912088
005 20180605073454.5
006 m o u
007 cr mn||||a|a||
008 190606s2014 xx obm 000 0 eng d
020 $a 9781321343199
035 $a (MiAaPQ)AAI3645362
035 $a (MiAaPQ)umn:15452
035 $a AAI3645362
040 $a MiAaPQ $b eng $c MiAaPQ
099 $a TUL $f hyy $c available through World Wide Web
100 1 $a Kotloski, Nicholas Jason. $3 1184284
245 1 0 $a Export and Role of Flavin Electron Shuttles in Shewanella oneidensis Strain MR-1.
264 0 $c 2014
300 $a 1 online resource (130 pages)
336 $a text $b txt $2 rdacontent
337 $a computer $b c $2 rdamedia
338 $a online resource $b cr $2 rdacarrier
500 $a Source: Dissertation Abstracts International, Volume: 76-03(E), Section: B.
500 $a Adviser: Jeffrey A. Gralnick.
502 $a Thesis (Ph.D.) $c University of Minnesota $d 2014.
504 $a Includes bibliographical references
520 $a Our planet is home to microbes with an impressive diversity of metabolic capabilities. For instance, dissimilatory metal reducing bacteria (DMRB) can respire minerals, heavy metals, and electrodes. Electrode respiration is important both for investigating DMRB and biotechnology applications including biosensors, bioenergy, and wastewater treatment. Placing electrodes in wastewater allows for electricity production and oxidation of carbon sources to carbon dioxide. Shewanella oneidensis MR-1, a model DMRB, transfers electrons generated from metabolism across the inner and outer membranes to insoluble external electron acceptors. This feat is accomplished using an electron conduit consisting of multi-heme cytochromes and flavin electron shuttles. The electron conduit in Shewanella evolved to move electrons out of the cell, but in controlled environments, the conduit is functionally reversible, meaning electrons can flow either out of or into the cell. In a process termed electrosynthesis, electrons are transferred from an electrode into a cell to direct metabolism to produce specific metabolites. A useful way to study electrosynthesis in cells is to use three-electrode bioreactors. The working electrode in a bioreactor is poised at an oxidizing or reducing potential and continuously measures electrons entering or leaving the electrode. When electrodes were used to donate electrons to attached Shewanella cells, intracellular ATP was produced. Mutants defective in generating or using proton motive force were unable to produce wild-type levels of ATP. If significant quantities of ATP can be produced by reverse electron flow, it will be a critical step towards fixing carbon dioxide into metabolic precursors and eventually into biofuels.
533 $a Electronic reproduction. $b Ann Arbor, Mich. : $c ProQuest, $d 2018
538 $a Mode of access: World Wide Web
650 4 $a Microbiology. $3 591510
655 7 $a Electronic books. $2 local $3 554714
690 $a 0410
710 2 $a ProQuest Information and Learning Co. $3 1178819
710 2 $a University of Minnesota. $b Microbiology, Immunology and Cancer Biology. $3 1184285
773 0 $t Dissertation Abstracts International $g 76-03B(E).
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3645362 $z click for full text (PQDT)