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Condition Dependent Tea-Sensitive Channels on Crayfish Motor Axon.

Record Type:	Language materials, manuscript : Monograph/item
Title/Author:	Condition Dependent Tea-Sensitive Channels on Crayfish Motor Axon./
Author:	Yu, Feiyuan.
Description:	1 online resource (46 pages)
Notes:	Source: Masters Abstracts International, Volume: 56-06.
Subject:	Biology. -
Online resource:	click for full text (PQDT)
ISBN:	9780355141092

Condition Dependent Tea-Sensitive Channels on Crayfish Motor Axon.
Yu, Feiyuan.

Condition Dependent Tea-Sensitive Channels on Crayfish Motor Axon. - 1 online resource (46 pages)

Source: Masters Abstracts International, Volume: 56-06.

Thesis (M.A.)--Boston University, 2017.

Includes bibliographical references

In previous studies, some channels, called the "sleeper channels," were reported to contribute to the shaping of the action potential (AP) only under non-physiological conditions. These channels have been hypothesized to play a role in providing a protective mechanism to prevent damage from neuronal hyperexcitation. Here we applied two-electrode current clamp at the primary branch point (1°BP) and the presynaptic terminal simultaneously on crayfish axons. Cadmium had minimal effects on AP shaping, suggesting the absence of calcium-activated potassium channels. Application of 1 mM TEA had minimal impact on AP waveform. In the presence of 4-Aminopyridine (4-AP), the same tetraethylammonium (TEA) concentration significantly prolonged AP duration, resembling the behaviors of sleeper channels. The kinetics of the TEA-sensitive channel (Kv(TEA)) is similar to the Kv2 family of mammalian K+ channels. TEA depolarized the potential after an AP and increased the AP duration in a dose-dependent manner, indicating that these channels contributed to maintaining AP waveform majorly during the hyperpolarization. The terminals were more sensitive to the blockers, suggesting a probability of regulation on neurotransmitter release. However, the TEA-sensitive channels at the crayfish axon had a higher affinity to TEA than the Kv2 channels. Pharmacological profiles, spatial distinction and function of the Kv(TEA) in the crayfish axon require further study.

Electronic reproduction.
Ann Arbor, Mich. :
ProQuest,
2018

Mode of access: World Wide Web

ISBN: 9780355141092Subjects--Topical Terms:

599573
Biology.
Index Terms--Genre/Form:

554714
Electronic books.

Condition Dependent Tea-Sensitive Channels on Crayfish Motor Axon.
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245 1 0 $a Condition Dependent Tea-Sensitive Channels on Crayfish Motor Axon.
264 0 $c 2017
300 $a 1 online resource (46 pages)
336 $a text $b txt $2 rdacontent
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500 $a Source: Masters Abstracts International, Volume: 56-06.
500 $a Adviser: Jen-Wei Lin.
502 $a Thesis (M.A.)--Boston University, 2017.
504 $a Includes bibliographical references
520 $a In previous studies, some channels, called the "sleeper channels," were reported to contribute to the shaping of the action potential (AP) only under non-physiological conditions. These channels have been hypothesized to play a role in providing a protective mechanism to prevent damage from neuronal hyperexcitation. Here we applied two-electrode current clamp at the primary branch point (1°BP) and the presynaptic terminal simultaneously on crayfish axons. Cadmium had minimal effects on AP shaping, suggesting the absence of calcium-activated potassium channels. Application of 1 mM TEA had minimal impact on AP waveform. In the presence of 4-Aminopyridine (4-AP), the same tetraethylammonium (TEA) concentration significantly prolonged AP duration, resembling the behaviors of sleeper channels. The kinetics of the TEA-sensitive channel (Kv(TEA)) is similar to the Kv2 family of mammalian K+ channels. TEA depolarized the potential after an AP and increased the AP duration in a dose-dependent manner, indicating that these channels contributed to maintaining AP waveform majorly during the hyperpolarization. The terminals were more sensitive to the blockers, suggesting a probability of regulation on neurotransmitter release. However, the TEA-sensitive channels at the crayfish axon had a higher affinity to TEA than the Kv2 channels. Pharmacological profiles, spatial distinction and function of the Kv(TEA) in the crayfish axon require further study.
533 $a Electronic reproduction. $b Ann Arbor, Mich. : $c ProQuest, $d 2018
538 $a Mode of access: World Wide Web
650 4 $a Biology. $3 599573
650 4 $a Neurosciences. $3 593561
655 7 $a Electronic books. $2 local $3 554714
690 $a 0306
690 $a 0317
710 2 $a ProQuest Information and Learning Co. $3 1178819
710 2 $a Boston University. $b Biology. $3 1185931
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=10266295 $z click for full text (PQDT)