國立虎尾科技大學 |

Combined Effects of Environmental Stressors and Crude Oil on Fundulus grandis Development and Transcriptome.

紀錄類型:	書目-語言資料,手稿 : Monograph/item
正題名/作者:	Combined Effects of Environmental Stressors and Crude Oil on Fundulus grandis Development and Transcriptome./
作者:	Serafin, Jennifer.
面頁冊數:	1 online resource (120 pages)
附註:	Source: Masters Abstracts International, Volume: 56-06.
標題:	Aquatic sciences. -
電子資源:	click for full text (PQDT)
ISBN:	9780355157703

Combined Effects of Environmental Stressors and Crude Oil on Fundulus grandis Development and Transcriptome.
Serafin, Jennifer.

Combined Effects of Environmental Stressors and Crude Oil on Fundulus grandis Development and Transcriptome. - 1 online resource (120 pages)

Source: Masters Abstracts International, Volume: 56-06.

Thesis (M.S.)--Purdue University, 2017.

Includes bibliographical references

Oil spills have polluted the marine environment for decades and continue to be a major source of polycyclic aromatic hydrocarbons (PAHs) to marine ecosystems around the globe. Although the toxicity of PAHs to fish has been well studied, the combined effects of extreme abiotic factors and oil are poorly understood. The overarching hypothesis tested in this work was that exposure to oil in combination with suboptimal environmental conditions will increase the sensitivity of fish larvae to PAHs. Gulf of Mexico killifish Fundulus grandis larvae (< 24 h post hatch) were exposed to varying environmental conditions (dissolved oxygen (DO) 2, 6 ppm; temperature 20, 25, 30°C; and salinity 3, 10, 30 ppt) combined with varying concentrations of high energy water accommodated fractions (HEWAF) (SigmaPAHs 0--125 ppb) for a total of 48 h. Larvae survival and development were negatively affected by PAHs, starting with the lowest concentration tested (15 ppb). High temperature + hypoxia + PAH resulted in the lowest survival and salinity had little impact on endpoints tested. Expression (via qPCR) of the hepatic detoxifying gene cyp1a was highly induced in PAH-exposed larvae, but only under normoxic conditions. Since hypoxia + PAHs resulted in significant negative effects, we next used RNASeq to evaluate the transcriptome of fish larvae in order to better understand the molecular mechanisms behind these effects. A total of 380 and 110 differentially expressed genes were identified due to PAH and hypoxia exposure, respectively. These expression changes were primarily associated with toxicological effects on the hepatic and cardiac systems. Ugt1a1, a detoxiying enzyme, was the only gene found up-regulated across all comparisons in response to PAHs and was identified as a potential biomarker of exposure to oil. The expression of cyp1a was not induced, in contrast to results from qPCR, but other genes associated with the aryl-hydrocarbon receptor (AhR) were ( ahrr, por). This work highlights the need for more studies examining the combined impact of suboptimal water quality parameters in the presence of pollution in fish early life-stages.

Electronic reproduction.
Ann Arbor, Mich. :
ProQuest,
2018

Mode of access: World Wide Web

ISBN: 9780355157703Subjects--Topical Terms:

1178821
Aquatic sciences.
Index Terms--Genre/Form:

554714
Electronic books.

Combined Effects of Environmental Stressors and Crude Oil on Fundulus grandis Development and Transcriptome.
LDR:03324ntm a2200325K 4500 001 913235
005 20180618102543.5
006 m o u
007 cr mn||||a|a||
008 190606s2017 xx obm 000 0 eng d
020 $a 9780355157703
035 $a (MiAaPQ)AAI10275318
035 $a (MiAaPQ)purdue:21444
035 $a AAI10275318
040 $a MiAaPQ $b eng $c MiAaPQ
100 1 $a Serafin, Jennifer. $3 1185975
245 1 0 $a Combined Effects of Environmental Stressors and Crude Oil on Fundulus grandis Development and Transcriptome.
264 0 $c 2017
300 $a 1 online resource (120 pages)
336 $a text $b txt $2 rdacontent
337 $a computer $b c $2 rdamedia
338 $a online resource $b cr $2 rdacarrier
500 $a Source: Masters Abstracts International, Volume: 56-06.
500 $a Adviser: Maria Sepulveda.
502 $a Thesis (M.S.)--Purdue University, 2017.
504 $a Includes bibliographical references
520 $a Oil spills have polluted the marine environment for decades and continue to be a major source of polycyclic aromatic hydrocarbons (PAHs) to marine ecosystems around the globe. Although the toxicity of PAHs to fish has been well studied, the combined effects of extreme abiotic factors and oil are poorly understood. The overarching hypothesis tested in this work was that exposure to oil in combination with suboptimal environmental conditions will increase the sensitivity of fish larvae to PAHs. Gulf of Mexico killifish Fundulus grandis larvae (< 24 h post hatch) were exposed to varying environmental conditions (dissolved oxygen (DO) 2, 6 ppm; temperature 20, 25, 30°C; and salinity 3, 10, 30 ppt) combined with varying concentrations of high energy water accommodated fractions (HEWAF) (SigmaPAHs 0--125 ppb) for a total of 48 h. Larvae survival and development were negatively affected by PAHs, starting with the lowest concentration tested (15 ppb). High temperature + hypoxia + PAH resulted in the lowest survival and salinity had little impact on endpoints tested. Expression (via qPCR) of the hepatic detoxifying gene cyp1a was highly induced in PAH-exposed larvae, but only under normoxic conditions. Since hypoxia + PAHs resulted in significant negative effects, we next used RNASeq to evaluate the transcriptome of fish larvae in order to better understand the molecular mechanisms behind these effects. A total of 380 and 110 differentially expressed genes were identified due to PAH and hypoxia exposure, respectively. These expression changes were primarily associated with toxicological effects on the hepatic and cardiac systems. Ugt1a1, a detoxiying enzyme, was the only gene found up-regulated across all comparisons in response to PAHs and was identified as a potential biomarker of exposure to oil. The expression of cyp1a was not induced, in contrast to results from qPCR, but other genes associated with the aryl-hydrocarbon receptor (AhR) were ( ahrr, por). This work highlights the need for more studies examining the combined impact of suboptimal water quality parameters in the presence of pollution in fish early life-stages.
533 $a Electronic reproduction. $b Ann Arbor, Mich. : $c ProQuest, $d 2018
538 $a Mode of access: World Wide Web
650 4 $a Aquatic sciences. $3 1178821
650 4 $a Toxicology. $3 580973
655 7 $a Electronic books. $2 local $3 554714
690 $a 0792
690 $a 0383
710 2 $a ProQuest Information and Learning Co. $3 1178819
710 2 $a Purdue University. $b Forestry and Natural Resources. $3 1181054
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=10275318 $z click for full text (PQDT)