國立虎尾科技大學 |

Monitoring of Vibrio Species in Oysters Crassostrea virginica and Seawater of Delaware Bay and Molecular Characterization of Vibrio parahaemolyticus.

Record Type:	Language materials, manuscript : Monograph/item
Title/Author:	Monitoring of Vibrio Species in Oysters Crassostrea virginica and Seawater of Delaware Bay and Molecular Characterization of Vibrio parahaemolyticus./
Author:	Almuhaideb, Esam.
Description:	1 online resource (105 pages)
Notes:	Source: Masters Abstracts International, Volume: 57-01.
Subject:	Food science. -
Online resource:	click for full text (PQDT)
ISBN:	9780355252699

Monitoring of Vibrio Species in Oysters Crassostrea virginica and Seawater of Delaware Bay and Molecular Characterization of Vibrio parahaemolyticus.
Almuhaideb, Esam.

Monitoring of Vibrio Species in Oysters Crassostrea virginica and Seawater of Delaware Bay and Molecular Characterization of Vibrio parahaemolyticus. - 1 online resource (105 pages)

Source: Masters Abstracts International, Volume: 57-01.

Thesis (M.S.)--Delaware State University, 2017.

Includes bibliographical references

Delaware Bay is the prime oystering ground in Delaware. Oysters naturally inhabit marine environments and, as filter-feeding mollusks, they tend to accumulate different microorganisms from seawater during their filtration process. Most of the illnesses caused by the consumption of raw and undercooked oysters are strongly associated with the oysters contaminated with Vibrio spp. Vibrio spp. are Gram-negative pathogenic bacteria from the family Vibrionaceae that negatively impact aquatic systems and human health. This study aimed to identify and differentiate Vibrio spp. in oyster and seawater samples from the Delaware Bay using CHROMagar(TM) Vibrio medium which provides the means for easy differentiation of Vibrio parahaemolyticus, Vibrio vulnificus, Vibrio cholerae, and Vibrio alginolyticus based on the colony color. Pathogenic Vibrio parahaemolyticus was characterized using conventional PCR which was applied for the identification of total and pathogenic V. parahaemolyticus by screening the species-specific tlh gene, and the virulent tdh, trh, toxR, and vpm genes.

Electronic reproduction.
Ann Arbor, Mich. :
ProQuest,
2018

Mode of access: World Wide Web

ISBN: 9780355252699Subjects--Topical Terms:

1179759
Food science.
Index Terms--Genre/Form:

554714
Electronic books.

Monitoring of Vibrio Species in Oysters Crassostrea virginica and Seawater of Delaware Bay and Molecular Characterization of Vibrio parahaemolyticus.
LDR:04705ntm a2200373K 4500 001 913331
005 20180618102624.5
006 m o u
007 cr mn||||a|a||
008 190606s2017 xx obm 000 0 eng d
020 $a 9780355252699
035 $a (MiAaPQ)AAI10619179
035 $a (MiAaPQ)desu:10056
035 $a AAI10619179
040 $a MiAaPQ $b eng $c MiAaPQ
100 1 $a Almuhaideb, Esam. $3 1186126
245 1 0 $a Monitoring of Vibrio Species in Oysters Crassostrea virginica and Seawater of Delaware Bay and Molecular Characterization of Vibrio parahaemolyticus.
264 0 $c 2017
300 $a 1 online resource (105 pages)
336 $a text $b txt $2 rdacontent
337 $a computer $b c $2 rdamedia
338 $a online resource $b cr $2 rdacarrier
500 $a Source: Masters Abstracts International, Volume: 57-01.
500 $a Adviser: Gulnihal Ozbay.
502 $a Thesis (M.S.)--Delaware State University, 2017.
504 $a Includes bibliographical references
520 $a Delaware Bay is the prime oystering ground in Delaware. Oysters naturally inhabit marine environments and, as filter-feeding mollusks, they tend to accumulate different microorganisms from seawater during their filtration process. Most of the illnesses caused by the consumption of raw and undercooked oysters are strongly associated with the oysters contaminated with Vibrio spp. Vibrio spp. are Gram-negative pathogenic bacteria from the family Vibrionaceae that negatively impact aquatic systems and human health. This study aimed to identify and differentiate Vibrio spp. in oyster and seawater samples from the Delaware Bay using CHROMagar(TM) Vibrio medium which provides the means for easy differentiation of Vibrio parahaemolyticus, Vibrio vulnificus, Vibrio cholerae, and Vibrio alginolyticus based on the colony color. Pathogenic Vibrio parahaemolyticus was characterized using conventional PCR which was applied for the identification of total and pathogenic V. parahaemolyticus by screening the species-specific tlh gene, and the virulent tdh, trh, toxR, and vpm genes.
520 $a Vibrio spp. were differentiated and isolated, and total and pathogenic V. parahaemolyticus from oyster and seawater samples were identified. Oysters and seawater samples were collected once a month from June through October 2016, from Bowers Beach, and Lewes, Broadkill in Delaware Bay. A third site, Slaughter Beach, was added to this study from August through October 2016. Physico-chemical water quality parameters recoded on-site during samples collection include water and air temperature, salinity, pH, dissolved oxygen, turbidity, chlorophyll a, depth, conductivity, weather, wind direction, wind speed, and tide.
520 $a Oyster and seawater samples were serially diluted up to (10--6 ), and 100 muL of each dilution from each sample were spread plated on CHROMagar(TM) Vibrio medium. V. parahaemolyticus, V. vulnificus/V. cholerae, and V. alginolyticus colonies were identified based on color development (mauve, green/blue, and colorless, respectively). Average Vibrio spp. in oyster ranged from <10 CFU g -1 (non-detectable) to 2.8x104 CFU g-1 , while the average of Vibrio spp. in seawater samples ranged from 1.7x10 CFU mL-1 to 4.47x103 CFU mL -1. More Vibrio colony forming units were observed during the warmer months (June through September). As anticipated, the total colony forming units of Vibrio spp. were proportional in both oyster and seawater samples. The multiple regression model indicated a significant positive relationship between Vibrio spp. and V. parahaemolyticus Log10 CFUs and water temperature, (p < 0.05). The one-way ANOVA analysis showed no statistical significant association between Vibrio spp. Log10 CFUs and the sample type, oysters and seawater ( p > 0.05).
520 $a A total of 165 bacterial isolates of Vibrio parahaemolyticus (mauve colonies) from oyster and seawater samples were examined for the presence of tlh, tdh, trh, toxR, and vpm genetic markers. Only 19% of the samples tested were positive for tdh and 24% were positive for trh while 83%, 65.5%, and 67% of the samples were positive for tlh, toxR, and vpm, respectively. This confirmed the specificity of tlh gene for V. parahaemolyticus species. Screening for tdh and trh genes is not sufficient for the surveillance of pathogenic or potential pathogenic V. parahaemolyticus but the reliability of toxR and vpm as gene markers is notably higher.
533 $a Electronic reproduction. $b Ann Arbor, Mich. : $c ProQuest, $d 2018
538 $a Mode of access: World Wide Web
650 4 $a Food science. $3 1179759
650 4 $a Microbiology. $3 591510
650 4 $a Molecular biology. $3 583443
655 7 $a Electronic books. $2 local $3 554714
690 $a 0359
690 $a 0410
690 $a 0307
710 2 $a ProQuest Information and Learning Co. $3 1178819
710 2 $a Delaware State University. $b Food Science and Biotechnology. $3 1186127
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=10619179 $z click for full text (PQDT)