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Effects of the Norepinephrine and Dopamine Reuptake Inhibitor, Bupropion and the Selective Serotonin Reuptake Inhibitor, Fluoxetine on Larval Zebrafish Locomotor Behavior and Development.

Record Type:	Language materials, manuscript : Monograph/item
Title/Author:	Effects of the Norepinephrine and Dopamine Reuptake Inhibitor, Bupropion and the Selective Serotonin Reuptake Inhibitor, Fluoxetine on Larval Zebrafish Locomotor Behavior and Development./
Author:	McMahon, Julia.
Description:	1 online resource (42 pages)
Notes:	Source: Masters Abstracts International, Volume: 57-01.
Subject:	Biology. -
Online resource:	click for full text (PQDT)
ISBN:	9780355242386

Effects of the Norepinephrine and Dopamine Reuptake Inhibitor, Bupropion and the Selective Serotonin Reuptake Inhibitor, Fluoxetine on Larval Zebrafish Locomotor Behavior and Development.
McMahon, Julia.

Effects of the Norepinephrine and Dopamine Reuptake Inhibitor, Bupropion and the Selective Serotonin Reuptake Inhibitor, Fluoxetine on Larval Zebrafish Locomotor Behavior and Development. - 1 online resource (42 pages)

Source: Masters Abstracts International, Volume: 57-01.

Thesis (M.S.)--Villanova University, 2017.

Includes bibliographical references

Pharmaceuticals and personal care products are entering waterways by slipping through wastewater treatment plants. Some of the most common pharmaceuticals include antidepressants, which have been found in the environment in concentrations ranging from 60 to over 2000 ng/L. These drugs create a therapeutic effect by altering levels of monoamines like serotonin and dopamine. Fluoxetine (commonly marketed as Prozac) a selective serotonin reuptake inhibitor (SSRI), and bupropion (commonly marketed as Wellbutrin) a norepinephrine and dopamine reuptake inhibitor (NDRI), work by inhibiting the reuptake of neurotransmitters by presynaptic neurons, effectively increasing the extracellular level of said neurotransmitter. As the name implies, SSRIs block the reuptake of serotonin while NDRIs block the reuptake of norepinephrine and dopamine. Both serotonin and dopamine are highly involved in locomotor control and development in vertebrates. Serotonin is necessary for the development of rhythmic motion, and dopamine imbalances are linked to movement disorders. In this study we tested whether exposure to bupropion or fluoxetine induces changes in the locomotor behavior of developing zebrafish.

Electronic reproduction.
Ann Arbor, Mich. :
ProQuest,
2018

Mode of access: World Wide Web

ISBN: 9780355242386Subjects--Topical Terms:

599573
Biology.
Index Terms--Genre/Form:

554714
Electronic books.

Effects of the Norepinephrine and Dopamine Reuptake Inhibitor, Bupropion and the Selective Serotonin Reuptake Inhibitor, Fluoxetine on Larval Zebrafish Locomotor Behavior and Development.
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245 1 0 $a Effects of the Norepinephrine and Dopamine Reuptake Inhibitor, Bupropion and the Selective Serotonin Reuptake Inhibitor, Fluoxetine on Larval Zebrafish Locomotor Behavior and Development.
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500 $a Source: Masters Abstracts International, Volume: 57-01.
500 $a Adviser: Angela DiBenedetto.
502 $a Thesis (M.S.)--Villanova University, 2017.
504 $a Includes bibliographical references
520 $a Pharmaceuticals and personal care products are entering waterways by slipping through wastewater treatment plants. Some of the most common pharmaceuticals include antidepressants, which have been found in the environment in concentrations ranging from 60 to over 2000 ng/L. These drugs create a therapeutic effect by altering levels of monoamines like serotonin and dopamine. Fluoxetine (commonly marketed as Prozac) a selective serotonin reuptake inhibitor (SSRI), and bupropion (commonly marketed as Wellbutrin) a norepinephrine and dopamine reuptake inhibitor (NDRI), work by inhibiting the reuptake of neurotransmitters by presynaptic neurons, effectively increasing the extracellular level of said neurotransmitter. As the name implies, SSRIs block the reuptake of serotonin while NDRIs block the reuptake of norepinephrine and dopamine. Both serotonin and dopamine are highly involved in locomotor control and development in vertebrates. Serotonin is necessary for the development of rhythmic motion, and dopamine imbalances are linked to movement disorders. In this study we tested whether exposure to bupropion or fluoxetine induces changes in the locomotor behavior of developing zebrafish.
520 $a Starting at 2 hours post fertilization (hpf) we randomly assigned embryos to one of three treatments: 0.2mM bupropion, 0.0046 mM fluoxetine, or 120 mM ( 0.05 %) ethanol. These concentrations, which are much higher than those found in the environment, were based on previous studies and used to ascertain effects on locomotor behavior. We examined the effect of drug exposure on three major locomotor mile markers during zebrafish development: spontaneous coiling, touch response, and sustained swimming. Spontaneous coiling, which appears at 24 hpf, is marked by the initiation of lateral flexion without body displacement. We observed individual embryos at 24 hpf and counted the number of spontaneous coiling episodes that occurred over 30 s. A response to tactile stimuli develops around 48 hpf and is characterized by a quick burst of swimming in the direction opposite the stimuli. We measured this response at 72 hpf (after hatching) by touching a probe to the tails of the larvae. Responses were categorized as swimming, flinching, or no response. Finally, sustained swimming develops around 4 dpf. Fish were placed in gridded petri dishes, and we video recorded swimming behavior in the absence of, and in the presence of prey at 5 dpf. We scored videos by counting the number of gridlines crossed by each fish in 60 s as a proxy measurement for distance. We found that motility in both the presence and absence of prey was reduced with drug treatment. While two clutches displayed anomalous results due to sample size, the trend was for bupropion treated larvae to cross fewer lines than the other two treatment groups. Bupropion treated fish also showed significantly fewer spontaneous coiling episodes in two of five clutches, and overall fewer swimming responses in the touch response test than other treatment groups. This study confirms that antidepressant exposure has the potential to alter locomotor behavior in developing zebrafish.
533 $a Electronic reproduction. $b Ann Arbor, Mich. : $c ProQuest, $d 2018
538 $a Mode of access: World Wide Web
650 4 $a Biology. $3 599573
655 7 $a Electronic books. $2 local $3 554714
690 $a 0306
710 2 $a ProQuest Information and Learning Co. $3 1178819
710 2 $a Villanova University. $b Biology. $3 1185987
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=10624261 $z click for full text (PQDT)