國立虎尾科技大學 |

Sensitivity to Hydrocarbons and Cytochrome P4501A Enzyme Activity in Arctic Marine Birds and Waterfowl.

紀錄類型:	書目-語言資料,手稿 : Monograph/item
正題名/作者:	Sensitivity to Hydrocarbons and Cytochrome P4501A Enzyme Activity in Arctic Marine Birds and Waterfowl./
作者:	Riddle-Berntsen, Ann E.
面頁冊數:	1 online resource (86 pages)
附註:	Source: Masters Abstracts International, Volume: 57-01.
標題:	Biology. -
電子資源:	click for full text (PQDT)
ISBN:	9780355538885

Sensitivity to Hydrocarbons and Cytochrome P4501A Enzyme Activity in Arctic Marine Birds and Waterfowl.
Riddle-Berntsen, Ann E.

Sensitivity to Hydrocarbons and Cytochrome P4501A Enzyme Activity in Arctic Marine Birds and Waterfowl. - 1 online resource (86 pages)

Source: Masters Abstracts International, Volume: 57-01.

Thesis (M.S.)--University of Alaska Fairbanks, 2017.

Includes bibliographical references

The Arctic is host to a taxonomically diverse group of birds, including species of conservation and subsistence importance that spend many months of their annual cycle in the region. With prospects for oil and gas resource development and increases in vessel traffic in the Beaufort and Chukchi Seas, arctic birds could be valuable bioindicators to monitor contaminants and specifically hydrocarbons from crude oil. Using liver cytochrome P4501A (CYP1A) activity, I measured levels of hydrocarbon exposure in three bird species of subsistence importance: king eiders (Somateria spectabilis ), common eiders (Somateria mollissima), and greater white-fronted geese (Anser albifrons). Over the course of three years, I collected liver samples during spring and fall hunts near Utqia?vik (formally Barrow) and validated methods for both direct-take and opportunistic liver sampling. Enzyme activity results show significant differences in CYP1A activity levels among species, seasons, and years. Except birds collected during fall 2014, when significantly high enzyme activity was observed in all sampled species, all other collections resulted in median activity levels similar to those reported in other sea duck species in Alaska from un-oiled or non-industrialized habitats. I also used species-specific hepatocyte culture in a broader selection of arctic marine birds and waterfowl candidate bioindicators to assess and compare species CYP1A activity responses as a measure of sensitivity to hydrocarbons. Cytochrome P4501A results from hepatocyte cultures dosed with positive control reference reagents and Alaska North Slope crude oil showed differences in species responses. Based on sensitivity results, I recommend the common eider and common murre (Uria aalge) as bioindicators for use in CYP1A monitoring due to their consistent and measureable responses in our experiments. However, additional species are promising candidates (e.g., tufted puffin; Fratercula cirrhata) but further testing is needed. This is the first study of reference hydrocarbon exposure and comparative laboratory assessment of CYP1A inducing compounds for arctic marine birds and waterfowl and these results form the basis for hydrocarbon monitoring programs and risk assessments.

Electronic reproduction.
Ann Arbor, Mich. :
ProQuest,
2018

Mode of access: World Wide Web

ISBN: 9780355538885Subjects--Topical Terms:

599573
Biology.
Index Terms--Genre/Form:

554714
Electronic books.

Sensitivity to Hydrocarbons and Cytochrome P4501A Enzyme Activity in Arctic Marine Birds and Waterfowl.
LDR:03409ntm a2200313K 4500 001 913370
005 20180618102637.5
006 m o u
007 cr mn||||a|a||
008 190606s2017 xx obm 000 0 eng d
020 $a 9780355538885
035 $a (MiAaPQ)AAI10641510
035 $a (MiAaPQ)uaf:10731
035 $a AAI10641510
040 $a MiAaPQ $b eng $c MiAaPQ
100 1 $a Riddle-Berntsen, Ann E. $3 1186183
245 1 0 $a Sensitivity to Hydrocarbons and Cytochrome P4501A Enzyme Activity in Arctic Marine Birds and Waterfowl.
264 0 $c 2017
300 $a 1 online resource (86 pages)
336 $a text $b txt $2 rdacontent
337 $a computer $b c $2 rdamedia
338 $a online resource $b cr $2 rdacarrier
500 $a Source: Masters Abstracts International, Volume: 57-01.
500 $a Adviser: Tuula Hollmen.
502 $a Thesis (M.S.)--University of Alaska Fairbanks, 2017.
504 $a Includes bibliographical references
520 $a The Arctic is host to a taxonomically diverse group of birds, including species of conservation and subsistence importance that spend many months of their annual cycle in the region. With prospects for oil and gas resource development and increases in vessel traffic in the Beaufort and Chukchi Seas, arctic birds could be valuable bioindicators to monitor contaminants and specifically hydrocarbons from crude oil. Using liver cytochrome P4501A (CYP1A) activity, I measured levels of hydrocarbon exposure in three bird species of subsistence importance: king eiders (Somateria spectabilis ), common eiders (Somateria mollissima), and greater white-fronted geese (Anser albifrons). Over the course of three years, I collected liver samples during spring and fall hunts near Utqia?vik (formally Barrow) and validated methods for both direct-take and opportunistic liver sampling. Enzyme activity results show significant differences in CYP1A activity levels among species, seasons, and years. Except birds collected during fall 2014, when significantly high enzyme activity was observed in all sampled species, all other collections resulted in median activity levels similar to those reported in other sea duck species in Alaska from un-oiled or non-industrialized habitats. I also used species-specific hepatocyte culture in a broader selection of arctic marine birds and waterfowl candidate bioindicators to assess and compare species CYP1A activity responses as a measure of sensitivity to hydrocarbons. Cytochrome P4501A results from hepatocyte cultures dosed with positive control reference reagents and Alaska North Slope crude oil showed differences in species responses. Based on sensitivity results, I recommend the common eider and common murre (Uria aalge) as bioindicators for use in CYP1A monitoring due to their consistent and measureable responses in our experiments. However, additional species are promising candidates (e.g., tufted puffin; Fratercula cirrhata) but further testing is needed. This is the first study of reference hydrocarbon exposure and comparative laboratory assessment of CYP1A inducing compounds for arctic marine birds and waterfowl and these results form the basis for hydrocarbon monitoring programs and risk assessments.
533 $a Electronic reproduction. $b Ann Arbor, Mich. : $c ProQuest, $d 2018
538 $a Mode of access: World Wide Web
650 4 $a Biology. $3 599573
655 7 $a Electronic books. $2 local $3 554714
690 $a 0306
710 2 $a ProQuest Information and Learning Co. $3 1178819
710 2 $a University of Alaska Fairbanks. $b Marine Biology. $3 1186184
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=10641510 $z click for full text (PQDT)