國立虎尾科技大學 |

ISOLATION AND CHARACTERIZATON OF CYTOCHALASIN D-RESISTANT VARIANTS OF A CHINESE HAMSTER OVARY CELL LINE.

Record Type:	Language materials, manuscript : Monograph/item
Title/Author:	ISOLATION AND CHARACTERIZATON OF CYTOCHALASIN D-RESISTANT VARIANTS OF A CHINESE HAMSTER OVARY CELL LINE./
Author:	GRUND, STEPHEN HOWARD.
Description:	1 online resource (277 pages)
Notes:	Source: Dissertation Abstracts International, Volume: 44-03, Section: B, page: 6990.
Subject:	Genetics. -
Online resource:	click for full text (PQDT)

ISOLATION AND CHARACTERIZATON OF CYTOCHALASIN D-RESISTANT VARIANTS OF A CHINESE HAMSTER OVARY CELL LINE.
GRUND, STEPHEN HOWARD.

ISOLATION AND CHARACTERIZATON OF CYTOCHALASIN D-RESISTANT VARIANTS OF A CHINESE HAMSTER OVARY CELL LINE. - 1 online resource (277 pages)

Source: Dissertation Abstracts International, Volume: 44-03, Section: B, page: 6990.

Thesis (Ph.D.)--Iowa State University, 1983.

Includes bibliographical references

An enrichment selection method was used to isolate Chinese hamster ovary (CHO) AK 412 variants resistant to 1 (mu)g/ml cytochalasin D (CD). The cytochalasin D-resistant (Cyd('R)) variant phenotype was thoroughly characterized by physiological, cytogenetic, morphological and biochemical techniques.

Electronic reproduction.
Ann Arbor, Mich. :
ProQuest,
2018

Mode of access: World Wide Web

Subjects--Topical Terms:

578972
Genetics.
Index Terms--Genre/Form:

554714
Electronic books.

ISOLATION AND CHARACTERIZATON OF CYTOCHALASIN D-RESISTANT VARIANTS OF A CHINESE HAMSTER OVARY CELL LINE.
LDR:03398ntm a2200325K 4500 001 914565
005 20180724085440.5
006 m o u
007 cr mn||||a|a||
008 190606s1983 xx obm 000 0 eng d
035 $a (MiAaPQ)AAI8316322
035 $a AAI8316322
040 $a MiAaPQ $b eng $c MiAaPQ
100 1 $a GRUND, STEPHEN HOWARD. $3 1187860
245 1 0 $a ISOLATION AND CHARACTERIZATON OF CYTOCHALASIN D-RESISTANT VARIANTS OF A CHINESE HAMSTER OVARY CELL LINE.
264 0 $c 1983
300 $a 1 online resource (277 pages)
336 $a text $b txt $2 rdacontent
337 $a computer $b c $2 rdamedia
338 $a online resource $b cr $2 rdacarrier
500 $a Source: Dissertation Abstracts International, Volume: 44-03, Section: B, page: 6990.
502 $a Thesis (Ph.D.)--Iowa State University, 1983.
504 $a Includes bibliographical references
520 $a An enrichment selection method was used to isolate Chinese hamster ovary (CHO) AK 412 variants resistant to 1 (mu)g/ml cytochalasin D (CD). The cytochalasin D-resistant (Cyd('R)) variant phenotype was thoroughly characterized by physiological, cytogenetic, morphological and biochemical techniques.
520 $a The Cyd('R)-variants were shown to possess a membrane lesion which confers CD resistance by reducing the uptake of CD from the extracellular medium. The change in membrane structure conferring CD resistance was correlated with several changes in membrane function, including: a generalized drug cross-resistance; hypersensitivity to a membrane specific drug (procaine); significant changes in cell surface morphology; differences in cellular adhesion properties; and a decrease in membrane pliability.
520 $a Cell surface proteins (glycoproteins) were radiolabeled by three different methods: (1) {('125)I}-lactoperoxidase catalyzed iodination; (2) labeling of surface galactose residues by {('3)H}-NaBH(,4)-galactose oxidase; and (3) metabolic labeling of cell surface glycoproteins with {('3)H}-fucose and {('3)H}-glucosamine. Autoradiograms from SDS-polyacrylamide gels of labeled membrane proteins revealed at least six differences between the Cyd('R)-variants and the CHO parent. In all cases, the labeled proteins migrated slightly slower in the variant, suggesting that increased glycosylation and/or some other modification of membrane glycoproteins is associated with CD-resistance.
520 $a Although no definitive genetic mechanism or gene product responsible for CD resistance was established, indirect evidence strongly suggests that CD resistance (and other characteristics associated with the Cyd('R)-phenotype) is mediated by gene amplification. The amplified gene exists in the form of a novel homogeneous staining region (HSR) on the long arm of chromosome 1.
520 $a The characteristics associated with the Cyd('R)-phenotype have been observed in many other drug-uptake and efflux mutants. A model is proposed that attempts to explain reduced drug uptake or increased drug efflux in terms of gene amplification. In this model, amplification of a gene(s) that controls glycosylation and/or phosphorylation levels of cell surface glycoproteins results in a pleiotropic phenotype.
533 $a Electronic reproduction. $b Ann Arbor, Mich. : $c ProQuest, $d 2018
538 $a Mode of access: World Wide Web
650 4 $a Genetics. $3 578972
655 7 $a Electronic books. $2 local $3 554714
690 $a 0369
710 2 $a ProQuest Information and Learning Co. $3 1178819
710 2 $a Iowa State University. $3 1184555
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=8316322 $z click for full text (PQDT)