國立虎尾科技大學 |

High-Throughput KASP DNA Genotyping to Select for Flowering Time among Progeny of Wild Cultivated Hybridization Populations of Chickpea.

紀錄類型:	書目-語言資料,手稿 : Monograph/item
正題名/作者:	High-Throughput KASP DNA Genotyping to Select for Flowering Time among Progeny of Wild Cultivated Hybridization Populations of Chickpea./
作者:	Suman, Shraddha.
面頁冊數:	1 online resource (42 pages)
附註:	Source: Masters Abstracts International, Volume: 56-06.
Contained By:	Masters Abstracts International56-06(E).
標題:	Plant sciences. -
電子資源:	click for full text (PQDT)
ISBN:	9780355149807

High-Throughput KASP DNA Genotyping to Select for Flowering Time among Progeny of Wild Cultivated Hybridization Populations of Chickpea.
Suman, Shraddha.

High-Throughput KASP DNA Genotyping to Select for Flowering Time among Progeny of Wild Cultivated Hybridization Populations of Chickpea. - 1 online resource (42 pages)

Source: Masters Abstracts International, Volume: 56-06.

Thesis (M.S.)--University of California, Davis, 2017.

Includes bibliographical references

Because of the progress made in human genome SNP genotyping, high-throughput SNP genotyping is increasingly being used in plant breeding for the past decade. This project focuses on the KASP assay, which combines FRET with allele-specific amplification, to track the status of a gene that governs the flowering time trait in the chickpea plant. The Cook laboratory has identified a large-effect QTL locus, designated Dtf3-1, on chickpea linkage group 3 that controls flowering time differences among wild and cultivated chickpea, wherein the QTL region encompasses a candidate gene. In other plant species, orthologs of this candidate gene, from the Flowering locus T (FT, for short) protein family have been shown to regulate the transition to flowering. In the case of chickpea, individuals possessing the cultivated FT allele (FTc) in either the heterozygous or homozygous condition flower earlier than those with only the wild FT allele (FTw). The allelic state of the FT locus can be monitored by molecular markers from the QTL region and the candidate FT locus, which provides an opportunity to identify early flowering genotypes even in the seedling stage. DNA from F2 chickpea seeds was extracted using a crude genomic DNA extraction procedure and genotyped for the status of the FT locus using the KASP assay. The seeds were then planted and the day to first flower for each plant was observed to see if the flowering time correlated with the predicted genotype. Leaf tissue DNA from the grown plants was used to reconfirm the genotypes. The Kruskal-Wallis test was used to verify the KASP assay as a genotype predictive method.

Electronic reproduction.
Ann Arbor, Mich. :
ProQuest,
2018

Mode of access: World Wide Web

ISBN: 9780355149807Subjects--Topical Terms:

1179743
Plant sciences.
Index Terms--Genre/Form:

554714
Electronic books.

High-Throughput KASP DNA Genotyping to Select for Flowering Time among Progeny of Wild Cultivated Hybridization Populations of Chickpea.
LDR:02927ntm a2200349Ki 4500 001 916767
005 20180928111500.5
006 m o u
007 cr mn||||a|a||
008 190606s2017 xx obm 000 0 eng d
020 $a 9780355149807
035 $a (MiAaPQ)AAI10265900
035 $a (MiAaPQ)ucdavis:16941
035 $a AAI10265900
040 $a MiAaPQ $b eng $c MiAaPQ $d NTU
100 1 $a Suman, Shraddha. $3 1190593
245 1 0 $a High-Throughput KASP DNA Genotyping to Select for Flowering Time among Progeny of Wild Cultivated Hybridization Populations of Chickpea.
264 0 $c 2017
300 $a 1 online resource (42 pages)
336 $a text $b txt $2 rdacontent
337 $a computer $b c $2 rdamedia
338 $a online resource $b cr $2 rdacarrier
500 $a Source: Masters Abstracts International, Volume: 56-06.
500 $a Adviser: Douglas Cook.
502 $a Thesis (M.S.)--University of California, Davis, 2017.
504 $a Includes bibliographical references
520 $a Because of the progress made in human genome SNP genotyping, high-throughput SNP genotyping is increasingly being used in plant breeding for the past decade. This project focuses on the KASP assay, which combines FRET with allele-specific amplification, to track the status of a gene that governs the flowering time trait in the chickpea plant. The Cook laboratory has identified a large-effect QTL locus, designated Dtf3-1, on chickpea linkage group 3 that controls flowering time differences among wild and cultivated chickpea, wherein the QTL region encompasses a candidate gene. In other plant species, orthologs of this candidate gene, from the Flowering locus T (FT, for short) protein family have been shown to regulate the transition to flowering. In the case of chickpea, individuals possessing the cultivated FT allele (FTc) in either the heterozygous or homozygous condition flower earlier than those with only the wild FT allele (FTw). The allelic state of the FT locus can be monitored by molecular markers from the QTL region and the candidate FT locus, which provides an opportunity to identify early flowering genotypes even in the seedling stage. DNA from F2 chickpea seeds was extracted using a crude genomic DNA extraction procedure and genotyped for the status of the FT locus using the KASP assay. The seeds were then planted and the day to first flower for each plant was observed to see if the flowering time correlated with the predicted genotype. Leaf tissue DNA from the grown plants was used to reconfirm the genotypes. The Kruskal-Wallis test was used to verify the KASP assay as a genotype predictive method.
533 $a Electronic reproduction. $b Ann Arbor, Mich. : $c ProQuest, $d 2018
538 $a Mode of access: World Wide Web
650 4 $a Plant sciences. $3 1179743
650 4 $a Agriculture. $3 660421
650 4 $a Molecular biology. $3 583443
655 7 $a Electronic books. $2 local $3 554714
690 $a 0479
690 $a 0473
690 $a 0307
710 2 $a ProQuest Information and Learning Co. $3 1178819
710 2 $a University of California, Davis. $b Forensic Science. $3 1190594
773 0 $t Masters Abstracts International $g 56-06(E).
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=10265900 $z click for full text (PQDT)