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High-Throughput KASP DNA Genotyping to Select for Flowering Time among Progeny of Wild Cultivated Hybridization Populations of Chickpea.

Record Type:	Language materials, manuscript : Monograph/item
Title/Author:	High-Throughput KASP DNA Genotyping to Select for Flowering Time among Progeny of Wild Cultivated Hybridization Populations of Chickpea./
Author:	Suman, Shraddha.
Description:	1 online resource (42 pages)
Notes:	Source: Masters Abstracts International, Volume: 56-06.
Contained By:	Masters Abstracts International56-06(E).
Subject:	Plant sciences. -
Online resource:	click for full text (PQDT)
ISBN:	9780355149807

High-Throughput KASP DNA Genotyping to Select for Flowering Time among Progeny of Wild Cultivated Hybridization Populations of Chickpea.
Suman, Shraddha.

High-Throughput KASP DNA Genotyping to Select for Flowering Time among Progeny of Wild Cultivated Hybridization Populations of Chickpea. - 1 online resource (42 pages)

Source: Masters Abstracts International, Volume: 56-06.

Thesis (M.S.)--University of California, Davis, 2017.

Includes bibliographical references

Because of the progress made in human genome SNP genotyping, high-throughput SNP genotyping is increasingly being used in plant breeding for the past decade. This project focuses on the KASP assay, which combines FRET with allele-specific amplification, to track the status of a gene that governs the flowering time trait in the chickpea plant. The Cook laboratory has identified a large-effect QTL locus, designated Dtf3-1, on chickpea linkage group 3 that controls flowering time differences among wild and cultivated chickpea, wherein the QTL region encompasses a candidate gene. In other plant species, orthologs of this candidate gene, from the Flowering locus T (FT, for short) protein family have been shown to regulate the transition to flowering. In the case of chickpea, individuals possessing the cultivated FT allele (FTc) in either the heterozygous or homozygous condition flower earlier than those with only the wild FT allele (FTw). The allelic state of the FT locus can be monitored by molecular markers from the QTL region and the candidate FT locus, which provides an opportunity to identify early flowering genotypes even in the seedling stage. DNA from F2 chickpea seeds was extracted using a crude genomic DNA extraction procedure and genotyped for the status of the FT locus using the KASP assay. The seeds were then planted and the day to first flower for each plant was observed to see if the flowering time correlated with the predicted genotype. Leaf tissue DNA from the grown plants was used to reconfirm the genotypes. The Kruskal-Wallis test was used to verify the KASP assay as a genotype predictive method.

Electronic reproduction.
Ann Arbor, Mich. :
ProQuest,
2018

Mode of access: World Wide Web

ISBN: 9780355149807Subjects--Topical Terms:

1179743
Plant sciences.
Index Terms--Genre/Form:

554714
Electronic books.

High-Throughput KASP DNA Genotyping to Select for Flowering Time among Progeny of Wild Cultivated Hybridization Populations of Chickpea.
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