國立虎尾科技大學 |

Development of novel fibrinogen-chitosan scaffold for wound healing.

Record Type:	Language materials, manuscript : Monograph/item
Title/Author:	Development of novel fibrinogen-chitosan scaffold for wound healing./
Author:	Yuan, Tony T.
Description:	1 online resource (134 pages)
Notes:	Source: Dissertation Abstracts International, Volume: 78-06(E), Section: B.
Contained By:	Dissertation Abstracts International78-06B(E).
Subject:	Biomedical engineering. -
Online resource:	click for full text (PQDT)
ISBN:	9781369440843

Development of novel fibrinogen-chitosan scaffold for wound healing.
Yuan, Tony T.

Development of novel fibrinogen-chitosan scaffold for wound healing. - 1 online resource (134 pages)

Source: Dissertation Abstracts International, Volume: 78-06(E), Section: B.

Thesis (Ph.D.)--The University of Texas at San Antonio, 2016.

Includes bibliographical references

Wound healing is a complex process that requires the coordination of numerous growth factor and cell types. Despite the advancement in biomaterial scaffolds, few new wound dressing scaffolds have reached the clinic. The purpose of this research was to develop a novel nanofibrous chitosan (CS)-fibrinogen (Fb) wound dressing, via electrospinning, that is capable of releasing platelet derived growth factor (PDGF) for the promotion of fibroblast migration and wound healing. Novel CS/Fb scaffold was developed as the combination of two different polymer solutions using a dual-spinneret electrospinner. First, scaffold composed of CS and polyethylene oxide (PEO) was electrospun. These scaffolds demonstrated a biphasic solubilization of PEO and followed by CS, which was a particularly unique release kinetic for the release of small molecules and drugs. In addition, increasing CS concentration showed superior inhibition of proliferation and attachment of S. aureus. CS/PEO scaffold was then electrospun in conjunction with pristine Fb to produce a novel CS/PEO/Fb nanofibrous scaffold. The CS/PEO/Fb scaffold demonstrated superb mechanical and homogenous chemical distribution of its polymeric components. In addition, the scaffold had excellent permeation properties to both oxygen and water. Finally, PDGF was successfully incorporated in the scaffold without significantly changing its bioactivity. Exposure to PDGF released from the scaffold for 48 hours, fibroblast migration was significantly higher than treatment with a single dose of 5 ng/mL PDGF. The results highlight the application of the current CS/PEO/Fb nanofibrous scaffold for use as a bioactive wound dressing for the promotion of fibroblast activity and healing.

Electronic reproduction.
Ann Arbor, Mich. :
ProQuest,
2018

Mode of access: World Wide Web

ISBN: 9781369440843Subjects--Topical Terms:

588770
Biomedical engineering.
Index Terms--Genre/Form:

554714
Electronic books.

Development of novel fibrinogen-chitosan scaffold for wound healing.
LDR:02968ntm a2200325Ki 4500 001 917181
005 20181009045509.5
006 m o u
007 cr mn||||a|a||
008 190606s2016 xx obm 000 0 eng d
020 $a 9781369440843
035 $a (MiAaPQ)AAI10249419
035 $a (MiAaPQ)utsa:12119
035 $a AAI10249419
040 $a MiAaPQ $b eng $c MiAaPQ $d NTU
100 1 $a Yuan, Tony T. $3 1191142
245 1 0 $a Development of novel fibrinogen-chitosan scaffold for wound healing.
264 0 $c 2016
300 $a 1 online resource (134 pages)
336 $a text $b txt $2 rdacontent
337 $a computer $b c $2 rdamedia
338 $a online resource $b cr $2 rdacarrier
500 $a Source: Dissertation Abstracts International, Volume: 78-06(E), Section: B.
500 $a Advisers: Joo L. Ong; Jonathan M. Stahl.
502 $a Thesis (Ph.D.)--The University of Texas at San Antonio, 2016.
504 $a Includes bibliographical references
520 $a Wound healing is a complex process that requires the coordination of numerous growth factor and cell types. Despite the advancement in biomaterial scaffolds, few new wound dressing scaffolds have reached the clinic. The purpose of this research was to develop a novel nanofibrous chitosan (CS)-fibrinogen (Fb) wound dressing, via electrospinning, that is capable of releasing platelet derived growth factor (PDGF) for the promotion of fibroblast migration and wound healing. Novel CS/Fb scaffold was developed as the combination of two different polymer solutions using a dual-spinneret electrospinner. First, scaffold composed of CS and polyethylene oxide (PEO) was electrospun. These scaffolds demonstrated a biphasic solubilization of PEO and followed by CS, which was a particularly unique release kinetic for the release of small molecules and drugs. In addition, increasing CS concentration showed superior inhibition of proliferation and attachment of S. aureus. CS/PEO scaffold was then electrospun in conjunction with pristine Fb to produce a novel CS/PEO/Fb nanofibrous scaffold. The CS/PEO/Fb scaffold demonstrated superb mechanical and homogenous chemical distribution of its polymeric components. In addition, the scaffold had excellent permeation properties to both oxygen and water. Finally, PDGF was successfully incorporated in the scaffold without significantly changing its bioactivity. Exposure to PDGF released from the scaffold for 48 hours, fibroblast migration was significantly higher than treatment with a single dose of 5 ng/mL PDGF. The results highlight the application of the current CS/PEO/Fb nanofibrous scaffold for use as a bioactive wound dressing for the promotion of fibroblast activity and healing.
533 $a Electronic reproduction. $b Ann Arbor, Mich. : $c ProQuest, $d 2018
538 $a Mode of access: World Wide Web
650 4 $a Biomedical engineering. $3 588770
655 7 $a Electronic books. $2 local $3 554714
690 $a 0541
710 2 $a ProQuest Information and Learning Co. $3 1178819
710 2 $a The University of Texas at San Antonio. $b Biomedical Engineering. $3 1191143
773 0 $t Dissertation Abstracts International $g 78-06B(E).
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=10249419 $z click for full text (PQDT)