語系:
繁體中文
English
說明(常見問題)
登入
回首頁
切換:
標籤
|
MARC模式
|
ISBD
Immunostimulatory Effects of Antigen...
~
ProQuest Information and Learning Co.
Immunostimulatory Effects of Antigen-Conjugated InP/ZnS Quantum Dot Nanoparticles in an Avian Model.
紀錄類型:
書目-語言資料,手稿 : Monograph/item
正題名/作者:
Immunostimulatory Effects of Antigen-Conjugated InP/ZnS Quantum Dot Nanoparticles in an Avian Model./
作者:
Lyle, Chris.
面頁冊數:
1 online resource (169 pages)
附註:
Source: Dissertation Abstracts International, Volume: 79-09(E), Section: B.
Contained By:
Dissertation Abstracts International79-09B(E).
標題:
Immunology. -
電子資源:
click for full text (PQDT)
ISBN:
9780355915518
Immunostimulatory Effects of Antigen-Conjugated InP/ZnS Quantum Dot Nanoparticles in an Avian Model.
Lyle, Chris.
Immunostimulatory Effects of Antigen-Conjugated InP/ZnS Quantum Dot Nanoparticles in an Avian Model.
- 1 online resource (169 pages)
Source: Dissertation Abstracts International, Volume: 79-09(E), Section: B.
Thesis (Ph.D.)--University of Arkansas, 2018.
Includes bibliographical references
Due to their unique physicochemical and enhanced immunostimulatory properties, quantum dot (QD) nanoparticles have shown increasing promise in biomedical research applications including bioimaging, drug delivery, and as vaccine adjuvants. Toxicity, however, remains a concern for the use of QD in these applications and thus, there is an increased demand for effective in vitro and in vivo systems to measure the bioactivity of QD. In this study in vitro and in vivo chicken models were used to investigate the effects of QD on innate and adaptive immunity. Chicken macrophage cultures were treated in vitro with QD to measure macrophage activation and the effect of QD on cell viability. The chicken growing feather (GF) injection model was used as the cutaneous test-site to monitor leukocyte infiltration in response to intradermal injection of QD without and with conjugation to a protein antigen. Additionally, the humoral and cellular adaptive immune responses to protein antigen conjugated to QD were examined in chickens. In vitro results showed negligible macrophage activation in response to QD treatment; however, cell viability was negatively affected by QD in a dose-dependent manner. Leukocyte infiltration results of in vivo GF injections with QD revealed immunostimulatory activity of QD, independent of whether or not QD were conjugated to protein antigen. When used as a vaccine platform, immunization with QD conjugated with protein antigen generated a higher primary and secondary antibody response compared to antigen mixed with alum adjuvant. The adaptive cellular responses to protein antigen examined in GF of immunized chickens also support an important role of B cells in the local effector phases of the responses, while T cells appear to primarily help in B cell activation and differentiation, including antibody isotype switching from IgM to IgG. These results provide additional evidence that QD possess promising vaccine adjuvant properties while demonstrating the viability and utility of the minimally invasive chicken GF cutaneous test-site to study the immunostimulatory effects of nanoparticles initiated in vivo. Further investigation of nanoparticles using the chicken GF injection model should provide additional valuable knowledge of the relative risks and rewards of using QD in biological applications.
Electronic reproduction.
Ann Arbor, Mich. :
ProQuest,
2018
Mode of access: World Wide Web
ISBN: 9780355915518Subjects--Topical Terms:
592892
Immunology.
Index Terms--Genre/Form:
554714
Electronic books.
Immunostimulatory Effects of Antigen-Conjugated InP/ZnS Quantum Dot Nanoparticles in an Avian Model.
LDR
:03616ntm a2200349Ki 4500
001
917457
005
20181012133446.5
006
m o u
007
cr mn||||a|a||
008
190606s2018 xx obm 000 0 eng d
020
$a
9780355915518
035
$a
(MiAaPQ)AAI10813694
035
$a
(MiAaPQ)uark:13005
035
$a
AAI10813694
040
$a
MiAaPQ
$b
eng
$c
MiAaPQ
$d
NTU
100
1
$a
Lyle, Chris.
$3
1191511
245
1 0
$a
Immunostimulatory Effects of Antigen-Conjugated InP/ZnS Quantum Dot Nanoparticles in an Avian Model.
264
0
$c
2018
300
$a
1 online resource (169 pages)
336
$a
text
$b
txt
$2
rdacontent
337
$a
computer
$b
c
$2
rdamedia
338
$a
online resource
$b
cr
$2
rdacarrier
500
$a
Source: Dissertation Abstracts International, Volume: 79-09(E), Section: B.
500
$a
Adviser: Gisela F. Erf.
502
$a
Thesis (Ph.D.)--University of Arkansas, 2018.
504
$a
Includes bibliographical references
520
$a
Due to their unique physicochemical and enhanced immunostimulatory properties, quantum dot (QD) nanoparticles have shown increasing promise in biomedical research applications including bioimaging, drug delivery, and as vaccine adjuvants. Toxicity, however, remains a concern for the use of QD in these applications and thus, there is an increased demand for effective in vitro and in vivo systems to measure the bioactivity of QD. In this study in vitro and in vivo chicken models were used to investigate the effects of QD on innate and adaptive immunity. Chicken macrophage cultures were treated in vitro with QD to measure macrophage activation and the effect of QD on cell viability. The chicken growing feather (GF) injection model was used as the cutaneous test-site to monitor leukocyte infiltration in response to intradermal injection of QD without and with conjugation to a protein antigen. Additionally, the humoral and cellular adaptive immune responses to protein antigen conjugated to QD were examined in chickens. In vitro results showed negligible macrophage activation in response to QD treatment; however, cell viability was negatively affected by QD in a dose-dependent manner. Leukocyte infiltration results of in vivo GF injections with QD revealed immunostimulatory activity of QD, independent of whether or not QD were conjugated to protein antigen. When used as a vaccine platform, immunization with QD conjugated with protein antigen generated a higher primary and secondary antibody response compared to antigen mixed with alum adjuvant. The adaptive cellular responses to protein antigen examined in GF of immunized chickens also support an important role of B cells in the local effector phases of the responses, while T cells appear to primarily help in B cell activation and differentiation, including antibody isotype switching from IgM to IgG. These results provide additional evidence that QD possess promising vaccine adjuvant properties while demonstrating the viability and utility of the minimally invasive chicken GF cutaneous test-site to study the immunostimulatory effects of nanoparticles initiated in vivo. Further investigation of nanoparticles using the chicken GF injection model should provide additional valuable knowledge of the relative risks and rewards of using QD in biological applications.
533
$a
Electronic reproduction.
$b
Ann Arbor, Mich. :
$c
ProQuest,
$d
2018
538
$a
Mode of access: World Wide Web
650
4
$a
Immunology.
$3
592892
650
4
$a
Nanotechnology.
$3
557660
650
4
$a
Animal sciences.
$3
1178863
655
7
$a
Electronic books.
$2
local
$3
554714
690
$a
0982
690
$a
0652
690
$a
0475
710
2
$a
ProQuest Information and Learning Co.
$3
1178819
710
2
$a
University of Arkansas.
$b
Poultry Science.
$3
1179846
773
0
$t
Dissertation Abstracts International
$g
79-09B(E).
856
4 0
$u
http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=10813694
$z
click for full text (PQDT)
筆 0 讀者評論
多媒體
評論
新增評論
分享你的心得
Export
取書館別
處理中
...
變更密碼[密碼必須為2種組合(英文和數字)及長度為10碼以上]
登入