國立虎尾科技大學 |

A Global View of Structure-Function Relationships in the Tautomerase Superfamily.

紀錄類型:	書目-語言資料,手稿 : Monograph/item
正題名/作者:	A Global View of Structure-Function Relationships in the Tautomerase Superfamily./
作者:	Davidson, Rebecca.
面頁冊數:	1 online resource (91 pages)
附註:	Source: Dissertation Abstracts International, Volume: 79-09(E), Section: B.
Contained By:	Dissertation Abstracts International79-09B(E).
標題:	Bioinformatics. -
電子資源:	click for full text (PQDT)
ISBN:	9780355857580

A Global View of Structure-Function Relationships in the Tautomerase Superfamily.
Davidson, Rebecca.

A Global View of Structure-Function Relationships in the Tautomerase Superfamily. - 1 online resource (91 pages)

Source: Dissertation Abstracts International, Volume: 79-09(E), Section: B.

Thesis (Ph.D.)--University of California, San Francisco, 2018.

Includes bibliographical references

In the genomic age, there is so much data that experimental validation on all of it is impractical. Therefore, computational methods need to be employed to probe this influx of sequence data. Even though we can only experimentally characterize a small amount of these proteins, the sequence data carries a tremendous amount of information through the genetic diversity introduced. This work is concerned with strategies that work towards harnessing sequence similarity networks to their fullest potential within the context of a particular enzyme superfamily, the Tautomerase Superfamily (TSF).

Electronic reproduction.
Ann Arbor, Mich. :
ProQuest,
2018

Mode of access: World Wide Web

ISBN: 9780355857580Subjects--Topical Terms:

583857
Bioinformatics.
Index Terms--Genre/Form:

554714
Electronic books.

A Global View of Structure-Function Relationships in the Tautomerase Superfamily.
LDR:03344ntm a2200373Ki 4500 001 918851
005 20181106104113.5
006 m o u
007 cr mn||||a|a||
008 190606s2018 xx obm 000 0 eng d
020 $a 9780355857580
035 $a (MiAaPQ)AAI10685102
035 $a (MiAaPQ)ucsf:11553
035 $a AAI10685102
040 $a MiAaPQ $b eng $c MiAaPQ $d NTU
100 1 $a Davidson, Rebecca. $3 1193288
245 1 2 $a A Global View of Structure-Function Relationships in the Tautomerase Superfamily.
264 0 $c 2018
300 $a 1 online resource (91 pages)
336 $a text $b txt $2 rdacontent
337 $a computer $b c $2 rdamedia
338 $a online resource $b cr $2 rdacarrier
500 $a Source: Dissertation Abstracts International, Volume: 79-09(E), Section: B.
500 $a Adviser: Patricia Babbitt.
502 $a Thesis (Ph.D.)--University of California, San Francisco, 2018.
504 $a Includes bibliographical references
520 $a In the genomic age, there is so much data that experimental validation on all of it is impractical. Therefore, computational methods need to be employed to probe this influx of sequence data. Even though we can only experimentally characterize a small amount of these proteins, the sequence data carries a tremendous amount of information through the genetic diversity introduced. This work is concerned with strategies that work towards harnessing sequence similarity networks to their fullest potential within the context of a particular enzyme superfamily, the Tautomerase Superfamily (TSF).
520 $a Sequence similarity networks (SSNs), are a type of similarity network in which the nodes represent proteins (or groups of similar proteins as in the representative networks) and the edges represent the similarity between two nodes. The edges are well defined and calculated as the pairwise BLAST E-value. These networks provide a graphical view of the similarity relationships within a set of proteins and provide a means to facilitate large-scale analyses. They can also be studied analytically using a variety of algorithms and allow the all-by-all comparisons of tens of thousands of proteins in an intuitively accessible manner.
520 $a In this work, a new method of probing SSNs in presented in application to the TSF. This method involves identifying a similarity path through a network with the sequences on the path exhibiting transitional functional features. This path was then used to guide target selection for crystallization of transition linker proteins. Those targets are presented in the context of larger bioinformatics analysis, including a phylogenetic reconstruction, and an experimental kinetic analysis.
520 $a Additionally, the classification of the TSF into subgroups, and in some cases a finer level of clustering, is provided. This curated work complete with sequence alignments and HMMs is hosted on the Structure Function Linkage Database for open access to the scientific community, with the alignments additionally available on GitHub.
533 $a Electronic reproduction. $b Ann Arbor, Mich. : $c ProQuest, $d 2018
538 $a Mode of access: World Wide Web
650 4 $a Bioinformatics. $3 583857
650 4 $a Biochemistry. $3 582831
655 7 $a Electronic books. $2 local $3 554714
690 $a 0715
690 $a 0487
710 2 $a ProQuest Information and Learning Co. $3 1178819
710 2 $a University of California, San Francisco. $b Biological and Medical Informatics. $3 1193289
773 0 $t Dissertation Abstracts International $g 79-09B(E).
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=10685102 $z click for full text (PQDT)